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Rabbit Monoclonal Lamin B1 antibody - conjugated to Alexa Fluor® 594. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication.


Images

Flow Cytometry - Alexa Fluor® 594 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (AB216723), expandable thumbnail

Publications

  • Frontiers in pharmacology 11:5692512020
    Inhibition of p38 MAPK Mitigates Lung Ischemia Reperfusion Injury by Reducing Blood-Air Barrier Hyperpermeability.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Tiantian Wang et. al.
    PubMed 33362540

Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

Alexa Fluor® 594

Excitation/Emission

Ex: 590nm, Em: 617nm

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt
Human
Tested
Mouse
Predicted
Rat
Predicted

Tested
Tested

Species

Human

Dilution info

1/50

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

11 products for Alternative Product

    5 products for Alternative Version

      Target data

      Function

      Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.

      Alternative names

      Lamin-B1, LMNB, LMN2, LMNB1

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      Rabbit Monoclonal Lamin B1 antibody - conjugated to Alexa Fluor® 594. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication.

      Alternative names

      Lamin-B1, LMNB, LMN2, LMNB1

      Key facts

      Isotype

      IgG

      Conjugation

      Alexa Fluor® 594

      Excitation/Emission

      Ex: 590nm, Em: 617nm

      Form

      Liquid

      Clonality

      Monoclonal

      Immunogen
      • The exact immunogen used to generate this antibody is proprietary information.
      Clone number

      EPR8985(B)

      Purification technique

      Affinity purification Protein A

      Concentration
      Loading...

      Storage

      Shipped at conditions

      Blue Ice

      Appropriate short-term storage duration

      1-2 weeks

      Appropriate short-term storage conditions

      +4°C

      Appropriate long-term storage conditions

      -20°C

      Aliquoting information

      Upon delivery aliquot

      Storage information

      Avoid freeze / thaw cycle, Store in the dark

      Notes

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free batch production

      For more information, read more on recombinant antibodies.

      Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

      Supplementary info

      Activity summary

      Lamin B1 also known as LMNB1 is a protein that plays an important role in the structure of the nuclear lamina a dense fibrillar network inside the nucleus of cells. This protein belongs to the lamin family and has an approximate molecular weight of 66 kDa. Lamin B1 is expressed in nearly all types of cells acting as a structural component by interacting with chromatin and anchoring the nuclear envelope. It contributes to regulating nuclear stability and mechanical support which is essential for proper cell function.

      Biological function summary

      Lamin B1 serves many important functions by providing structural integrity and regulating gene expression through chromatin organization. Part of the nuclear lamina complex Lamin B1 interacts with other lamin proteins such as Lamin A and Lamin C forming a mesh-like structure that supports the nuclear envelope. This protein also affects cellular processes such as DNA replication RNA transcription and cell division indicating its broad impact on various cellular activities.

      Pathways

      Lamin B1 plays an important role in gene expression regulation and cell cycle control. It interacts with pathways involved in chromatin remodeling influencing the access of transcription factors to DNA which affects gene expression profiles. Lamin B1 associates with related proteins such as Lamin A/C and other nuclear matrix proteins to maintain cellular architecture stability and normal cell function. This organization is vital to ensure the accuracy of cellular processes and prevent anomalies during cell division.

      Associated diseases and disorders

      Mutations or altered expression of Lamin B1 have been associated with several pathological conditions. One noteworthy disorder is autosomal dominant adult-onset leukodystrophy where Lamin B1 expression levels are abnormal. This protein also connects to multiple sclerosis through its role in maintaining nuclear architecture. Additionally changes in Lamin B1 expression levels can influence pathways involving Lamin A/C and potentially lead to other laminopathies highlighting its influence in maintaining cellular health and contributing to disease development.

      Product promise

      We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

      In the unlikely event of one of our products not working as expected, you are covered by our product promise.

      Full details and terms and conditions can be found here:
      Terms & Conditions.

      1 product image

      • Flow Cytometry - Alexa Fluor® 594 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab216723), expandable thumbnail

        Flow Cytometry - Alexa Fluor® 594 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab216723)

        Overlay histogram showing A431 cells stained with ab216723 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab216723, 1/50 dilution) for 30 min at 22°C.

        Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor® 594 (Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab208568) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

        Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 610/20 bandpass filter.

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      Product protocols

      For this product, it's our understanding that no specific protocols are required. You can:

      Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

      For licensing inquiries, please contact partnerships@abcam.com