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AB302684

Alexa Fluor® 594 Anti-LAMP1 antibody [EPR21026] - Lysosome Marker

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(2 Publications)

Rabbit Recombinant Monoclonal LAMP1 antibody - conjugated to Alexa Fluor® 594. Suitable for Flow Cyt (Intra), ICC/IF and reacts with Mouse samples. Cited in 2 publications.

View Alternative Names

CD107a, Lamp-1, Lamp1, Lysosome-associated membrane glycoprotein 1, LAMP-1, Lysosome-associated membrane protein 1, 120 kDa lysosomal membrane glycoprotein, CD107 antigen-like family member A, LGP-120, Lysosomal membrane glycoprotein A, P2B, LGP-A

2 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-LAMP1 antibody [EPR21026] - Lysosome Marker (AB302684)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-LAMP1 antibody [EPR21026] - Lysosome Marker (AB302684)

Immunofluorescent analysis of 4% paraformaldehyde fixed, 0.1% TritonX-100 permeabilized Neuro-2a (mouse neuroblastoma neuroblast) labeling LAMP1 with ab302684 at 1/50 dilution (10 μg/ml) (Red). Confocal image showing cytoplasmic staining in Neuro-2a cells.  ab195887 anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) (Green) was used as a counterstain at 1 : 200 dilution (2.5 μg/ml). Nuclear counter stain is DAPI (Blue).

Flow Cytometry (Intracellular) - Alexa Fluor® 594 Anti-LAMP1 antibody [EPR21026] - Lysosome Marker (AB302684)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 594 Anti-LAMP1 antibody [EPR21026] - Lysosome Marker (AB302684)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed and 90% methanol permeabilized Neuro-2a (mouse neuroblastoma neuroblast) labeling LAMP1 with ab302684 at 1/500 dilution (0.1 μg) (Red) compared with Rabbit monoclonal isotype control - Alexa Fluor® 594 (Black) and cells without incubation with primary antibody and secondary antibody (Blue).

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-LAMP1 antibody [EPR21026] - Lysosome Marker

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-LAMP1 antibody [EPR21026] - Lysosome Marker

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-LAMP1 antibody [EPR21026] - Lysosome Marker

  • 578 PE

    PE Anti-LAMP1 antibody [EPR21026] - Lysosome Marker

  • Unconjugated

    Anti-LAMP1 antibody [EPR21026] - Lysosome Marker

  • Carrier free

    Anti-LAMP1 antibody [EPR21026] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21026

Isotype

IgG

Conjugation

Alexa Fluor® 594

Excitation/Emission

Ex: 590nm, Em: 617nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LAMP1 also known as lysosome-associated membrane glycoprotein 1 is an important player within cellular mechanics. It exhibits a molecular weight of approximately 120 kDa. This protein exists abundantly in lysosomal membranes and sometimes in endosomes. LAMP1 operates near most cell types and has a significant presence in immune neuronal and epithelial cells. By maintaining the lysosomal membrane's integrity LAMP1 contributes to cellular homeostasis.
Biological function summary

LAMP1 functions to protect lysosomal membranes from the harsh environment inside the lysosome. It forms part of a protective glycocalyx composed of highly glycosylated proteins supporting lysosomal stability. This protein interacts closely with other lysosome markers and assists in the fusion of vesicles with lysosomes making it fundamental to lysosomal processes. As a lysosome marker LAMP1 helps identify lysosomal compartments during studies involving microscopy and LAMP1 staining.

Pathways

The protein LAMP1 participates in the autophagy and endocytic pathways. It acts collaboratively within the lysosomal degradation route involving proteins like LAMP2 which also supports lysosomal function. LAMP1 influences these pathways by mediating the fusion of autophagosomes or endosomes with lysosomes thereby ensuring efficient breakdown of cellular debris and macromolecules.

LAMP1 associates with lysosomal storage disorders and neurodegenerative diseases. The abnormal function or expression of LAMP1 has been linked to conditions such as Niemann-Pick disease and Alzheimer's disease. In these contexts its interaction with proteins like APP (amyloid precursor protein) illustrates its involvement in pathogenic pathways that lead to cellular dysfunction and disease progression. Understanding LAMP1's role may enhance diagnostic and therapeutic approaches pertaining to these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Lysosomal membrane glycoprotein which plays an important role in lysosome biogenesis, lysosomal pH regulation, autophagy and cholesterol homeostasis (PubMed : 15121881). Acts as an important regulator of lysosomal lumen pH regulation by acting as a direct inhibitor of the proton channel TMEM175, facilitating lysosomal acidification for optimal hydrolase activity (By similarity). Also plays an important role in NK-cells cytotoxicity (PubMed : 23847195). Mechanistically, participates in cytotoxic granule movement to the cell surface and perforin trafficking to the lytic granule (By similarity). In addition, protects NK-cells from degranulation-associated damage induced by their own cytotoxic granule content (PubMed : 23847195). Presents carbohydrate ligands to selectins (By similarity). Also implicated in tumor cell metastasis (PubMed : 2676155).
See full target information Lamp1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadv8640 PubMed40512859

2025

Interaction between NF-κB and PLAC8 impairs autophagy providing a survival advantage to prostate cells transformed by cadmium.

Applications

Unspecified application

Species

Unspecified reactive species

Vaibhav Shukla,Ashish Tyagi,Balaji Chandrasekaran,Bhawna Tyagi,Balpreet Singh,Thulasidharan Nair Devanarayanan,Venkatesh Kolluru,Murali K Ankem,Chendil Damodaran

Nature communications 16:3429 PubMed40210885

2025

Genetic modulation of rare earth nanoparticle biotransformation shapes biological outcomes.

Applications

Unspecified application

Species

Unspecified reactive species

Mingming Tian,Di Wu,Xiao Gou,Ruibin Li,Xiaowei Zhang
View all publications
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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