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AB281931

Alexa Fluor® 594 Anti-LAMP2A antibody [EPR4207(2)] - Lysosome Marker

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Rabbit Recombinant Monoclonal LAMP2 antibody - conjugated to Alexa Fluor® 594. Suitable for IHC-P and reacts with Human samples.

View Alternative Names

CD107b, Lysosome-associated membrane glycoprotein 2, LAMP-2, Lysosome-associated membrane protein 2, CD107 antigen-like family member B, LGP-96, LAMP2

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-LAMP2A antibody [EPR4207(2)] - Lysosome Marker (AB281931)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-LAMP2A antibody [EPR4207(2)] - Lysosome Marker (AB281931)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling LAMP2A with ab281931 at 1/50 dilution (10.0 μg/ml).

Positive staining on human liver.

The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab281931 at 1/50 dilution (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Counterstained with DAPI.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-LAMP2A antibody [EPR4207(2)] - Lysosome Marker (AB281931)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-LAMP2A antibody [EPR4207(2)] - Lysosome Marker (AB281931)

Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling LAMP2A with ab281931 at 1/50 dilution (10.0 μg/ml).

Negative control : no staining on human skeletal muscle.

The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab281931 at 1/50 dilution (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Counterstained with DAPI.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-LAMP2A antibody [EPR4207(2)] - Lysosome Marker (AB281931)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-LAMP2A antibody [EPR4207(2)] - Lysosome Marker (AB281931)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling LAMP2A with ab281931 at 1/50 dilution (10.0 μg/ml).

Negative control : no staining on human cerebrum.

The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab281931 at 1/50 dilution (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Counterstained with DAPI.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-LAMP2A antibody [EPR4207(2)] - Lysosome Marker (AB281931)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-LAMP2A antibody [EPR4207(2)] - Lysosome Marker (AB281931)

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue labeling LAMP2A with ab281931 at 1/50 dilution (10.0 μg/ml).

Positive staining on human prostate carcinoma.

The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab281931 at 1/50 dilution (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Counterstained with DAPI.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-LAMP2A antibody [EPR4207(2)] - Lysosome Marker

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-LAMP2A antibody [EPR4207(2)] - Lysosome Marker

  • 578 PE

    PE Anti-LAMP2A antibody [EPR4207(2)] (Lysosome Marker)

  • 660 APC

    APC Anti-LAMP2A antibody [EPR4207(2)] (Lysosome Marker)

  • HRP

    HRP Anti-LAMP2A antibody [EPR4207(2)] (Lysosome Marker)

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-LAMP2A antibody [EPR4207(2)]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-LAMP2A antibody [EPR4207(2)] - Lysosome Marker

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-LAMP2A antibody [EPR4207(2)] - Lysosome Marker

  • Unconjugated

    Anti-LAMP2A antibody [EPR4207(2)] - Lysosome Marker

  • Carrier free

    Anti-LAMP2A antibody [EPR4207(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4207(2)

Isotype

IgG

Conjugation

Alexa Fluor® 594

Excitation/Emission

Ex: 590nm, Em: 617nm

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LAMP2A also known as lysosome-associated membrane protein 2A functions as a lysosomal marker and plays a role in the lysosome size and structure regulation. Its molecular weight is approximately 45 kDa. This protein is expressed mainly in tissues with high metabolic activity such as liver kidney and heart. LAMP2A is an integral component of the lysosomal membrane and is important for the proper function and maintenance of lysosomes which are cellular organelles involved in the degradation and recycling of macromolecules.
Biological function summary

LAMP2A facilitates chaperone-mediated autophagy (CMA) a specific mechanism for the selective degradation of proteins. It is not part of a larger complex but rather acts independently within the lysosomal membrane. This transport functions through recognizing cytosolic proteins containing a KFERQ-like motif which are then translocated into the lysosome for degradation. LAMP2A upholds cellular homeostasis and is important in adapting to cellular stress by facilitating the turnover of damaged or unnecessary proteins.

Pathways

LAMP2A is intimately involved in the CMA pathway. This pathway is important for regulating protein quality control often linked with other autophagic processes. LAMP2A also interacts with the heat shock cognate protein (HSC70) which assists in unfolding and transporting substrate proteins to lysosomes. Through these interactions LAMP2A helps maintain cellular proteostasis which is essential for cellular function and survival especially during stress conditions.

LAMP2A is connected to lysosomal storage disorders such as Danon disease. This disease is characterized by the accumulation of autophagic vacuoles due to defects in lysosomal function. LAMP2A mutations alter lysosomal degradation capabilities leading to severe cardiomyopathy and myopathy. Additionally LAMP2A has implications in neurodegenerative diseases like Parkinson's disease due to its role in protein degradation pathways. In these contexts malfunction or altered expression of LAMP2A can disrupt cellular clearance processes contributing to pathogenesis along with proteins such as alpha-synuclein.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The protein expressed by gene LAMP2 plays an important role in chaperone-mediated autophagy, facilitating the lysosomal degradation of proteins in response to various stresses and as part of normal protein turnover, especially for those with a long biological half-life. It functions by binding target proteins like GAPDH and MLLT11, directing them for lysosomal degradation, and is necessary for the fusion of autophagosomes with lysosomes during autophagy. Although cells lacking LAMP2 express normal VAMP8 levels, they fail to accumulate STX17 on autophagosomes, likely explaining the fusion failure between autophagosomes and lysosomes. LAMP2 is essential for the normal degradation of autophagosomal contents and efficient MHCII-mediated presentation of exogenous antigens, as it facilitates lysosomal protein degradation. It is not required for the presentation of endogenous antigens via MHCII. Specifically, isoform LAMP-2C modulates chaperone-mediated autophagy, reduces presentation of endogenous antigens by MHCII, and does not influence the presentation of exogenous and membrane-derived antigens by MHCII. This supplementary information is collated from multiple sources and compiled automatically.
See full target information LAMP2
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Product promise

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