Alexa Fluor® 594 Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control Suitable for ICC/IF and reacts with samples.
Images
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (AB178000), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-594-mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab178000--immunocytochemistry-immunofluorescence-img65225.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (AB178000)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Conjugation
- Alexa Fluor® 594
- Excitation/Emission
- Ex: 590nm, Em: 617nm
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ICC/IF | Reactivity Reacts | Dilution info - | Notes - |
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Alexa Fluor® 594 Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control Suitable for ICC/IF and reacts with samples.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Conjugation
- Alexa Fluor® 594
- Excitation/Emission
- Ex: 590nm, Em: 617nm
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Clone number
- 15-6E10A7
- Purification technique
- Precipitation Ammonium Sulphate
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
Notes
Isotype controls are used to confirm that the primary antibody binding is specific and not a result of non-specific Fc receptor binding or other protein interactions. The isotype control antibody should match the primary antibody's host species, isotype, and possible conjugation. The Control performed appropriately in all materials and platforms that were tested.
Product was previously marketed under the MitoSciences sub-brand.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab178000), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-594-mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab178000--immunocytochemistry-immunofluorescence-img65225.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab178000)")
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab178000)
Immunocytochemistry data for ab178000. All panels show HeLa cells stained with DAPI as well as; a no primary control (A), ab178000 at 0.1 μg/mL (B), an Alexa 594 anti-SDHA conjugated antibody (Alexa Fluor® 594 Anti-SDHA antibody [2E3GC12FB2AE2] ab170172) at 0.1 μg/mL (C).
Cells were PFA fixed and stored in 0.02%azide/PBS in 4°C, then blocked with 10%GS for 2 hours, primary was diluted in 10%GS and incubated for 2 hours, cells were washed 3x with 1%GS and DAPI was added for 10 minutes in 1%GS.
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