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AB178002

Alexa Fluor® 594 Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control

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Alexa Fluor® 594 Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control Suitable for ICC/IF and reacts with samples.
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (AB178002)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (AB178002)

Immunocytochemistry data for ab178002. All panels show HeLa cells stained with DAPI as well as; a no primary control (A), ab178002 at 1 μg/mL (B), an Alexa 594 anti-SDHA conjugated antibody (ab170172) at 1 μg/mL (C).

Cells were PFA fixed and stored in 0.02%azide/PBS in 4°C. Cells were blocked with 10%GS for 2 hours, primary was diluted in 10%GS and incubated for 2 hours, cells were washed 3X with 1%GS and DAPI was added for 10 minutes in 1%GS.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

7E10G10

Isotype

IgG2b

Light chain type

kappa

Conjugation

Alexa Fluor® 594

Excitation/Emission

Ex: 590nm, Em: 617nm

Carrier free

No

Applications

ICC/IF

applications

Specificity

ab178002 was raised in mouse against a yeast-specific protein making it unsuitable for yeast experiments. ab178002 is a suitable control for Human, Rat, Mouse, Monkey, others untested.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Isotype controls are used to confirm that the primary antibody binding is specific and not a result of non-specific Fc receptor binding or other protein interactions. The isotype control antibody should match the primary antibody's host species, isotype, and possible conjugation. The Control performed appropriately in all materials and platforms that were tested.

Product was previously marketed under the MitoSciences sub-brand.

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Properties and storage information

Form
Liquid
Purification technique
Precipitation Ammonium Sulphate
Storage buffer
Preservative: 0.02% Sodium azide Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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