Alexa Fluor® 594 Anti-Myc tag antibody [9E10]

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling HBV envelope L+M protein with ab322399 at 1/4000 (0.129 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).

Confocal image showing cytoplasmic staining in 293T cells transfected with Hepatitis B virus genotype A2 subtype adw2 (strain Rutter 1979) Middle envelope protein expression vector containing a myc-His-tag® (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 5 ug/ml dilution (Magenta).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Hepatitis B Virus Core Antigen with ab323646 at 1/1000 (0.488 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green).

Confocal image showing cytoplasmic and nuclear staining in 293T cells transfected with a Virus wild-type Hepatitis B Virus Core Antigen expression vector containing a Myc tag (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 (5ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2ug/ml) dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling PER2 with ab325020 at 1/200 (2.46 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/ab150081 1000 2ug/ml dilution (Green).

Confocal image showing positive staining in 293T cells transfected with a mouse PER2 expression vector containing a myc-His-tag® (shown in green), and no staining in 293T cells transfected with a mouse PER1 or PER3 expression vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/ab223894 100 5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/ab150081 1000 2ug/ml dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Angiogenin with ab324046 at 1/50 (9.9 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/ab150081 1000 2ug/ml dilution (Green).

Confocal image showing cytoplasmic staining in 293T cells (shown in green) transfected with a human ANG expression vector containing a Myc tage and no staining in 293T cells transfected with empty vector or mouse ANG2, ANG3, ANG4 expression vector containing a Myc tage. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/ab223894 100 5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/ab150081 1000 2ug/ml dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling PLCZ1 with ab325528 at 1/5000 (0.103 μg/ml) dilution , followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing positive staining in 293T cells (shown in green) transfected with a human PLCZ1 vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T (human epithelial cell line from embryonic kidney) transfected with myc-tagged ACE2 expression vector cells labelling ACE2 with ab314771 at 1/500 (1.092 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing cytoplasmic staining in 293T cells transfected with ACE2 protein expression vector containing a myc tag, no staining on 293T transfected with empty vector containing a myc-tag. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T (human epithelial cell line from embryonic kidney) transfected with myc-tagged AGER expression vector cells labelling RAGE with ab314773 at 1/500 (1.034 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing membranous or cytoplasmic staining in 293T cells transfected with AGER protein expression vector containing a myc tag, no staining on 293T transfected with an empty vector containing a myc-tag.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T cells transfected with an empty expression vector containing a myc-His-tag^®; HEK-293T cells transfected with a mouse SFTPC expression vector containing a myc-His-tag^®; HEK-293T cells transfected with a human SFTPC expression vector containing a myc-His-tag^® cells labeling Prosurfactant Protein C with ab259667 at 1/5000 dilution following ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (Green).

Confocal image showing cytoplasmic staining in 293T cells (shown in green) transfected a mouse SFTPC expression vectorcontaining a myc-His-tag^® and 293T cells transfected a human SFTPC expression vector containing a myc-His-tag^®. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor^® 594) was used to counterstain at 1/100 dilution (Magenta). The nuclear counterstain was DAPI (Blue).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Tau (O-GlcNAc S400) with ab320076 at 1/500 (1.02 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

Confocal image showing positive staining in 293T cells transfected with a human Tau 2N4R expression vector containing a myc-His-tag®, and the signal increased after treatment with Thiamet G (10 um) for 48 h. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
A : 293T (human embryonic kidney epithelial cell) cells transfected with a human wild-type Tau 2N4R expression vector containing a myc-His-tag®
B : 293T cells transfected with a human wild-type Tau 2N4R expression vector containing a myc-His-tag® were treated with 10 μM Thiamet G for 48 hours
C : 293T cells transfected with a human Tau 2N4R (S400A mutation) expression vector containing a myc-His-tag®
D : 293T cells transfected with a human Tau 2N4R (S400A mutation) expression vector containing a myc-His-tag® were treated with 10 μM Thiamet G for 48 hours

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

ab223894 staining Myc-tag (pseudocolored in red) in 293T cells transfected with TMEM119 with myc tag. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab223894 at 1/100 dilution and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue) and Alexa Fluor^® 647 conjugated control Myc-tag antibody (different clone) is pseudocolored in white.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling MERS-CoV Spike glycoprotein with ab317040 at 1/1000 (1.03 ug/ml) dilution, followed by Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor® 488) antibody at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing cytoplasmic and membranous staining in 293T cells transfected with a MERS-CoV Spike glycoprotein protein expression vector containing a myc-His-tag® (shown in green) but no staining in 293T cells transfected with a SARS-CoV-2 Spike glycoprotein vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 (5ug/ml) dilution (Magenta). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor® 488) at 1/1000 (2 ug/ml dilution).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Influenza A Virus PB2 protein with ab320835 at 1/2000 (0.248 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

Confocal image showing positive staining in 293T cells (shown in green) transfected with an Influenza A Virus PB2 protein expression vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling OSMR with ab323715 at 1/100 (5.08 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).

Confocal image showing cytoplasmic staining in 293T cells (shown in green) transfected with a human OSMR expression vector containing a Myc tag. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling HBV envelope L protein with ab322461 at 1/50 (10.12 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/ 1000 2ug/ml dilution (Green).

Confocal image showing cytoplasmic staining in 293T cells (shown in green) transfected with a Hepatitis B virus genotype A2 subtype adw2 (strain Rutter 1979) Large (L) envelope protein expression vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/ 1000 2ug/ml dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling CD163 with ab322551 at 1/5000 (0.1 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).

Confocal image showing cytoplasmic staining in 293T cells (shown in green) transfected with a human CD163 expression vector containing a myc-His-tag®, showing no staining in 293T cells transfected with a human CD163L or empty expression vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/ab223894 100 5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling AGRP with ab323797 at 1/50 (10.02 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).

Confocal image showing cytoplasmic staining in 293T cells (shown in green) transfected with a human AGRP expression vector containing a Myc tag. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling groEL with ab318970 at 1/2000 (0.251 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

Confocal image showing positive staining in 293T cells transfected with an E.coli K12 Chaperonin GroEL expression vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling IL-7 with ab324645 at 1/1000 (0.507 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing positive staining in 293T (shown in green) cells transfected with a mouse Interleukin-7 or a human Interleukin-7 expression vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 (5 ug/ml) dilution (Magenta).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Hepatitis B Virus Surface Antigen with ab324674 at 1/2000 (0.229 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing positive staining in 293T cells (shown in green) transfected with a Hepatitis B virus genotype A2 subtype adw2 (strain Rutter 1979) Large (L) protein, Medium (M) protein and Small (S) protein expression vector containing a myc-His-tag® respectively. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Panel A : 293T cells transfected with an empty expression vector containing a myc-His-tag®.
Panel B : 293T cells transfected with a Hepatitis B virus genotype A2 subtype adw2 (strain Rutter 1979) Large (L) protein expression vector containing a myc-His-tag®.
Panel C : 293T cells transfected with a Hepatitis B virus genotype A2 subtype adw2 (strain Rutter 1979) Medium (M) protein expression vector containing a myc-His-tag®.
Panel D : 293T cells transfected with a Hepatitis B virus genotype A2 subtype adw2 (strain Rutter 1979) Small (S) protein expression vector containing a myc-His-tag®.

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 (5 ug/ml) dilution (Magenta).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Dengue NS3 with ab324565 at 1/1000 (0.482 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/ab150081 1000 2ug/ml dilution (Green).

Confocal image showing cytoplasmic staining in 293T cells transfected with a Dengue type 2 NS3 expression vector containing a myc-His-tag® (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/ab223894 100 5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/ab150081 1000 2ug/ml dilution.

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Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) transfected with an empty vector containing a Myc tag; 293T transfected with a mouse SFTPC containing a Myc tag cells labeling Prosurfactant Protein C with ab322443 at 1/500 dilution following ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (Green).

Confocal image showing membrane and cytoplasmic staining in 293T cells transfected with a mouse SFTPC containing a Myc tag (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor^® 594) was used to counterstain at 1/100 dilution (Magenta).

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Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) transfected with an empty vector containing a Myc tag; 293T transfected with a mouse SFTPC containing a Myc tag cells labeling Prosurfactant Protein C with ab312850 at 1/500 dilution following ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (Green).

Confocal image showing membrane and cytoplasmic staining in 293T cells transfected with a mouse SFTPC containing a Myc tag (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor^® 594) was used to counterstain at 1/100 dilution (Magenta).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) transfected with an empty vector containing a Myc tag; 293T transfected with a human SFTPC containing a Myc tag cells labeling Prosurfactant Protein C with ab312850 at 1/500 dilution following ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (Green).

Confocal image showing membrane and cytoplasmic staining in 293T cells transfected with a human SFTPC containing a Myc tag (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor^® 594) was used to counterstain at 1/100 dilution (Magenta).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) transfected with an empty vector containing a Myc tag; 293T transfected with a human SFTPC containing a Myc tag cells labeling Prosurfactant Protein C with ab322443 at 1/500 dilution following ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (Green).

Confocal image showing membrane and cytoplasmic staining in 293T cells transfected with a human SFTPC containing a Myc tag (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor^® 594) was used to counterstain at 1/100 dilution (Magenta).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T(human embryonic kidney epithelial cell)cells transfected with a mouse Olig1 expression vector containing a myc-His-tag® labelling Olig1 with ab325492 at 1/200 (2.59 µg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing positive staining in 293T cells (shown in green) transfected a mouse Olig1 expression vector containing a myc-His-tag®, showing no staining in 293T cells transfected a mouse Olig2/3 expression vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Panel A : HEK-293T cells transfected with an empty expression vector containing a myc-His-tag®;
Panel B : HEK-293T cells transfected with a mouse Olig1 expression vector containing a myc-His-tag®;
Panel C : HEK-293T cells transfected with a mouse Olig2 expression vector containing a myc-His-tag®;
Panel D : HEK-293T cells transfected with a mouse Olig3 expression vector containing a myc-His-tag®

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Dengue Virus 2 envelope glycoprotein with ab325012 at 1/5000 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing positive staining in 293T cells (shown in green) transfected with a Dengue Virus type 2 envelope glycoprotein expression vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) transfected with an empty vector containing a myc tag 293T transfected with a Virus Zika virus envelope protein E expression vector containing a myc tag 293T transfected with a Tick-borne encephalitis virus envelope protein E expression vector containing a myc tag 293T cells transfected with a Yellow fever virus envelope protein E expression vector containing a myc tag 293T transfected with a Dengue virus type 4 envelope protein E expression vector containing a myc tag 293T transfected with a Dengue virus type 3 envelope protein E expression vector containing a myc tag 293T transfected with a Virus Japanese encephalitis virus envelope protein E expression vector containing a myc tag 293T transfected with a West Nile virus envelope protein E expression vector containing a myc tag cells labelling Zika virus envelope protein E with ab325556 at 1/1000 (0.534 µg/ml) dilution , followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing cytoplasmic staining in 293T cells transfected with a Virus Zika virus envelope protein E expression vector containing a myc tag (shown in green), and no staining in 293T cells tranfected with empty vector, Tick-borne encephalitis virus envelope protein E, Yellow fever virus envelope protein E, Dengue virus type 4 envelope protein E, Dengue virus type 3 envelope protein E, Virus Japanese encephalitis virus envelope protein E or West Nile virus envelope protein E expression vectors containing a myc tag. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). A. 293T (human embryonic kidney epithelial cell) transfected with an empty vector containing a myc tag B. 293T transfected with a Virus Zika virus envelope protein E expression vector containing a myc tag C. 293T transfected with a Tick-borne encephalitis virus envelope protein E expression vector containing a myc tag D. 293T cells transfected with a Yellow fever virus envelope protein E expression vector containing a myc tag E. 293T transfected with a Dengue virus type 4 envelope protein E expression vector containing a myc tag F. 293T transfected with a Dengue virus type 3 envelope protein E expression vector containing a myc tag G. 293T transfected with a Virus Japanese encephalitis virus envelope protein E expression vector containing a myc tag H. 293T transfected with a West Nile virus envelope protein E expression vector containing a myc tag

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.