Alexa Fluor® 594 Anti-Myc tag antibody [9E10]

14 product images

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (ab223894)

  ab223894 staining Myc-tag (pseudocolored in red) in 293T cells transfected with TMEM119 with myc tag. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab223894 at 1/100 dilution and Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue) and Alexa Fluor^® 647 conjugated control Myc-tag antibody (different clone) is pseudocolored in white.
  

  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (ab223894)

  Myc tag Immunocytochemistry/ Immunofluorescence staining using mouse Anti-Myc tag antibody

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling AGRP with Anti-AGRP antibody [EPR22984-1] ab323797 at 1/50 (10.02 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).

  Confocal image showing cytoplasmic staining in 293T cells (shown in green) transfected with a human AGRP expression vector containing a Myc tag. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (ab223894)

  Myc tag Immunocytochemistry/ Immunofluorescence staining using mouse Anti-Myc tag antibody

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling OSMR with Anti-OSMR antibody [EPR29920-534] ab323715 at 1/100 (5.08 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).

  Confocal image showing cytoplasmic staining in 293T cells (shown in green) transfected with a human OSMR expression vector containing a Myc tag. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (ab223894)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Hepatitis B Virus Core Antigen with Anti-Hepatitis B Virus Core Antigen antibody [EPR29890-38] ab323646 at 1/1000 (0.488 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green).

  Confocal image showing cytoplasmic and nuclear staining in 293T cells transfected with a Virus wild-type Hepatitis B Virus Core Antigen expression vector containing a Myc tag (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 (5ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2ug/ml) dilution.

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (ab223894)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling HBV envelope L+M protein with Anti-HBV envelope L+M protein antibody [EPR29888-33] ab322399 at 1/4000 (0.129 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 1000 2ug/ml dilution (Green).

  Confocal image showing cytoplasmic staining in 293T cells transfected with Hepatitis B virus genotype A2 subtype adw2 (strain Rutter 1979) Middle envelope protein expression vector containing a myc-His-tag® (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/ab223894 100 5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 1000 2ug/ml dilution.

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (ab223894)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling CD163 with Anti-CD163 antibody [EPR29058-507] ab322551 at 1/5000 (0.1 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).

  Confocal image showing cytoplasmic staining in 293T cells (shown in green) transfected with a human CD163 expression vector containing a myc-His-tag®, showing no staining in 293T cells transfected with a human CD163L or empty expression vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/ab223894 100 5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (ab223894)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling HBV envelope L protein with Anti-Hepatitis B Virus Surface Antigen antibody [EPR29887-532] ab322461 at 1/50 (10.12 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/ 1000 2ug/ml dilution (Green).

  Confocal image showing cytoplasmic staining in 293T cells (shown in green) transfected with a Hepatitis B virus genotype A2 subtype adw2 (strain Rutter 1979) Large (L) envelope protein expression vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/ 1000 2ug/ml dilution.

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (ab223894)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Tau (O-GlcNAc S400) with Anti-Tau (O-GlcNAc S400) antibody [EPR24891-48] ab320076 at 1/500 (1.02 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

  Confocal image showing positive staining in 293T cells transfected with a human Tau 2N4R expression vector containing a myc-His-tag®, and the signal increased after treatment with Thiamet G (10 um) for 48 h. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
  
  A : 293T (human embryonic kidney epithelial cell) cells transfected with a human wild-type Tau 2N4R expression vector containing a myc-His-tag®
  
  B : 293T cells transfected with a human wild-type Tau 2N4R expression vector containing a myc-His-tag® were treated with 10 μM Thiamet G for 48 hours
  
  C : 293T cells transfected with a human Tau 2N4R (S400A mutation) expression vector containing a myc-His-tag®
  
  D : 293T cells transfected with a human Tau 2N4R (S400A mutation) expression vector containing a myc-His-tag® were treated with 10 μM Thiamet G for 48 hours

  ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (ab223894)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Influenza A Virus PB2 protein with Anti-Influenza A Virus PB2 protein antibody [EPR29356-84] ab320835 at 1/2000 (0.248 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

  Confocal image showing positive staining in 293T cells (shown in green) transfected with an Influenza A Virus PB2 protein expression vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (ab223894)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling MERS-CoV Spike glycoprotein with Anti-MERS-CoV Spike glycoprotein antibody [abd146] ab317040 at 1/1000 (1.03 ug/ml) dilution, followed by Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor® 488) antibody at 1/1000 (2 ug/ml) dilution (Green).

  Confocal image showing cytoplasmic and membranous staining in 293T cells transfected with a MERS-CoV Spike glycoprotein protein expression vector containing a myc-His-tag® (shown in green) but no staining in 293T cells transfected with a SARS-CoV-2 Spike glycoprotein vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
  
  Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

  ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 (5ug/ml) dilution (Magenta). The nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor® 488) at 1/1000 (2 ug/ml dilution).

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (ab223894)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling groEL with Anti-groEL antibody [EPR28718-8] ab318970 at 1/2000 (0.251 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

  Confocal image showing positive staining in 293T cells transfected with an E.coli K12 Chaperonin GroEL expression vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
  
  Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

  ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (ab223894)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T (human epithelial cell line from embryonic kidney) transfected with myc-tagged AGER expression vector cells labelling RAGE with Anti-RAGE antibody [RM1078] ab314773 at 1/500 (1.034 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).
  Confocal image showing membranous or cytoplasmic staining in 293T cells transfected with AGER protein expression vector containing a myc tag, no staining on 293T transfected with an empty vector containing a myc-tag.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
  ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (ab223894)

  Myc tag Immunocytochemistry/ Immunofluorescence staining using mouse Anti-Myc tag antibody

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Angiogenin with Anti-Angiogenin antibody [EPR29570-513] ab324046 at 1/50 (9.9 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 1000 2ug/ml dilution (Green).

  Confocal image showing cytoplasmic staining in 293T cells (shown in green) transfected with a human ANG expression vector containing a Myc tage and no staining in 293T cells transfected with empty vector or mouse ANG2, ANG3, ANG4 expression vector containing a Myc tage. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/ab223894 100 5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 1000 2ug/ml dilution.

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (ab223894)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T (human epithelial cell line from embryonic kidney) transfected with myc-tagged ACE2 expression vector cells labelling ACE2 with Anti-ACE2 antibody [RM1070] ab314771 at 1/500 (1.092 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).
  Confocal image showing cytoplasmic staining in 293T cells transfected with ACE2 protein expression vector containing a myc tag, no staining on 293T transfected with empty vector containing a myc-tag. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.