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Rabbit Recombinant Monoclonal Myelin oligodendrocyte glycoprotein antibody - conjugated to Alexa Fluor® 594. Suitable for IHC-P, IHC-Fr and reacts with Human, Mouse, Rat samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (AB305372), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (AB305372), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (AB305372), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (AB305372), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (AB305372), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

Alexa Fluor® 594

Excitation/Emission

Ex: 590nm, Em: 617nm

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIHC-Fr
Human
Tested
Expected
Mouse
Tested
Tested
Rat
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/50

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

1/50

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/50

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Mouse

Dilution info

1/50

Notes

-

Species

Rat

Dilution info

1/50

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

3 products for Alternative Version

    Target data

    Function

    Mediates homophilic cell-cell adhesion (By similarity). Minor component of the myelin sheath. May be involved in completion and/or maintenance of the myelin sheath and in cell-cell communication.(Microbial infection) Acts as a receptor for rubella virus.

    Alternative names

    Myelin-oligodendrocyte glycoprotein, MOG

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    Rabbit Recombinant Monoclonal Myelin oligodendrocyte glycoprotein antibody - conjugated to Alexa Fluor® 594. Suitable for IHC-P, IHC-Fr and reacts with Human, Mouse, Rat samples.

    Alternative names

    Myelin-oligodendrocyte glycoprotein, MOG

    Key facts

    Isotype

    IgG

    Conjugation

    Alexa Fluor® 594

    Excitation/Emission

    Ex: 590nm, Em: 617nm

    Form

    Liquid

    Clonality

    Monoclonal

    Immunogen
    • The exact immunogen used to generate this antibody is proprietary information.
    Clone number

    EPR22629-310

    Purification technique

    Affinity purification Protein A

    Concentration
    Loading...

    Storage

    Shipped at conditions

    Blue Ice

    Appropriate short-term storage duration

    1-2 weeks

    Appropriate short-term storage conditions

    +4°C

    Appropriate long-term storage conditions

    -20°C

    Aliquoting information

    Upon delivery aliquot

    Storage information

    Avoid freeze / thaw cycle, Store in the dark

    Notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free batch production

    For more information, read more on recombinant antibodies.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    Supplementary info

    Activity summary

    Myelin oligodendrocyte glycoprotein (MOG) also known as MOG protein or MOG glycoprotein is a lesser-known but important component of the central nervous system myelin. The MOG protein has a molecular mass of approximately 26 to 28 kDa. You will find it expressed on the surface of myelin sheaths and oligodendrocytes. This protein plays a role in the myelination process acting as a potential adhesive molecule or signaling molecule contributing to the stability and integrity of the myelin structure. Oligodendrocyte staining techniques can help visualize the distribution and expression patterns of MOG making it important for research purposes.

    Biological function summary

    MOG influences the immune response and possibly adhesion between myelin membranes. Although it does not form part of a larger well-defined complex it may interact with other myelin-associated proteins to contribute to myelin construction and maintenance. MOG's involvement in these processes supports myelin sheath formation and function aiding electrical conduction in nerve cells. The protein's exposure on the myelin membrane makes it a possible target for immune attacks hinting at its role in autoimmunity.

    Pathways

    MOG is involved in immune responses and central nervous system pathways. One significant pathway is the autoimmune pathway where MOG can engage with or be targeted by autoimmune antibodies. It interacts with other proteins like myelin basic protein (MBP) in maintaining myelin structures and influencing immunological functions. This interaction implies that disturbances in these pathways might contribute to numerous neurological disorders.

    Associated diseases and disorders

    MOG plays a critical role in conditions such as multiple sclerosis and neuromyelitis optica. In multiple sclerosis MOG is a target of autoantibodies leading to demyelination and loss of neural function. In neuromyelitis optica MOG antibodies may contribute to severe inflammation and damage in optic nerves and spinal cord. The connection between MOG and these diseases highlights associations with other proteins like aquaporin-4 which are similarly targeted in associated autoimmune responses. Understanding these relationships is key for developing diagnostics or therapies involving MOG ELISA methods to detect MOG-specific antibodies in patient samples.

    Product promise

    We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

    In the unlikely event of one of our products not working as expected, you are covered by our product promise.

    Full details and terms and conditions can be found here:
    Terms & Conditions.

    6 product images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab305372), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab305372)

      Image A: composite multiplex immunohistochemistry staining using conjugates panel composed of anti- GFAP (Alexa Fluor® 488) (Alexa Fluor® 488 Anti-GFAP antibody [EPR1034Y] ab194324, 1/100 dilution, 5 µg/mL) (green), anti-MOG (Alexa Fluor® 594) (ab305372, 1/100 dilution, 5 µg/mL) (magenta) and anti-Iba1 (Alexa Fluor® 647) (Alexa Fluor® 647 Anti-Iba1 antibody [EPR16588] ab225261, 1/100 dilution, 5 µg/mL) (white) on Formalin/PFA-fixed paraffin-embedded sections of rat cerebellum. Nuclear DNA was labelled with DAPI (blue).

      Image B: Formalin/PFA-fixed paraffin-embedded section of rat cerebellum stained with anti- GFAP (Alexa Fluor® 488) (Alexa Fluor® 488 Anti-GFAP antibody [EPR1034Y] ab194324, 1/100 dilution, 5 µg/mL).

      Image C: Formalin/PFA-fixed paraffin-embedded section of rat cerebellum stained with anti- MOG (Alexa Fluor® 594) (ab305372, 1/100 dilution, 5 µg/mL).

      Image D: Formalin/PFA-fixed paraffin-embedded section of rat cerebellum stained with anti- Iba1 (Alexa Fluor® 647) (Alexa Fluor® 647 Anti-Iba1 antibody [EPR16588] ab225261, 1/100 dilution, 5 µg/mL).

      Immunohistochemistry staining was performed on a Leica Biosystems BOND® RX instrument. Heat mediated antigen retrieval with EDTA (pH 9.0, 110°C) for 40 mins. The section was then incubated at room temperature for 1 hour and mounted using Dako Fluorescence Mounting Medium®.

      Image acquisition was performed with Leica SP8 confocal microscope. For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab305372), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab305372)

      Immunohistochemical analysis of paraffin-embedded Rat cerebellum tissue labeling Myelin oligodendrocyte glycoprotein with ab305372 at 1/50 (10.0 µg
      /ml). Positive staining on rat cerebellum.The section was incubated with ab305372 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND RX instrument.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins
      Negative control: PBS instead of the primary antibody

    • Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab305372), expandable thumbnail

      Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab305372)

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebellum (fresh) tissue labeling Myelin oligodendrocyte glycoprotein with ab305372 at 1/500 (1.0 µg/ml) dilution. Confocal image showing positive staining on mouse cerebellum. The section was incubated with ab305372 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Negative control: PBS instead of the primary antibody.

    • Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab305372), expandable thumbnail

      Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab305372)

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebellum (fresh) tissue labeling Myelin oligodendrocyte glycoprotein with ab305372 at 1/500 (1.0 µg/ml) dilution. Confocal image showing positive staining on rat cerebellum. The section was incubated with ab305372 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Negative control: PBS instead of the primary antibody.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab305372), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab305372)

      Immunohistochemical analysis of paraffin-embedded Human cerebellum tissue labeling Myelin oligodendrocyte glycoprotein with ab305372 at 1/50 (10.0 µg
      /ml). Positive staining on human cerebellum.The section was incubated with ab305372 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND RX instrument.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins
      Negative control: PBS instead of the primary antibody

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab305372), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab305372)

      Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labeling Myelin oligodendrocyte glycoprotein with ab305372 at 1/50 (10.0 µg
      /ml). Positive staining on mouse cerebellum.The section was incubated with ab305372 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND RX instrument.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins
      Negative control: PBS instead of the primary antibody

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