Skip to main content

Rabbit Recombinant Monoclonal NCAM1 antibody - conjugated to Alexa Fluor® 594. Suitable for ICC/IF, Flow Cyt and reacts with Human samples.

Be the first to review this product! Submit a review

Images

Flow Cytometry - Alexa Fluor® 594 Anti-NCAM1 antibody [EPR26939-108] (AB319185), expandable thumbnail
  • Flow Cytometry - Alexa Fluor® 594 Anti-NCAM1 antibody [EPR26939-108] (AB319185), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-NCAM1 antibody [EPR26939-108] (AB319185), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
Alexa Fluor® 594
Excitation/Emission
Ex: 590nm, Em: 617nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFFlow Cyt
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Target data

Function

This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. (Microbial infection) Acts as a receptor for rabies virus. (Microbial infection) Acts as a receptor for Zika virus.

Alternative names

CD56, NCAM, NCAM1, Neural cell adhesion molecule 1, N-CAM-1, NCAM-1

Recommended products

Rabbit Recombinant Monoclonal NCAM1 antibody - conjugated to Alexa Fluor® 594. Suitable for ICC/IF, Flow Cyt and reacts with Human samples.

Key facts

Isotype
IgG
Conjugation
Alexa Fluor® 594
Excitation/Emission
Ex: 590nm, Em: 617nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR26939-108
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

NCAM1 also known as Neural Cell Adhesion Molecule 1 or CD56 is an important cell surface glycoprotein that plays a role in cell-cell adhesion. It has a molecular mass of approximately 120-140 kDa. NCAM1 is widely expressed in various tissues including the nervous system skeletal muscle and certain hematopoietic cells. In flow cytometry and immunohistochemistry NCAM1's expression is extensively studied to track and analyze cell populations using techniques like NCAM1 ELISA for quantification.

Biological function summary

NCAM1 influences neuronal growth survival and synaptic plasticity. This protein is part of the immunoglobulin superfamily and contributes to dynamic interactions during development. NCAM1 exists on cell membranes assisting in the formation and maintenance of tissue structures. In particular cell adhesion molecule CAL53 interacts with NCAM1 highlighting its involvement in synaptic connections. Additionally NCAM1 impacts cellular migration and differentiation particularly in myogenic processes involving MY31's pathway.

Pathways

The NCAM1 protein participates in the MAPK signaling pathway and the PI3K/AKT pathway both essential in cellular growth and survival. NCAM1 acts alongside ERIC1 aiding in the transduction of signals that regulate cytoskeletal rearrangement. These pathways influence cellular communication and responses to external stimuli further enhancing tissue development and regeneration mechanisms.

Associated diseases and disorders

NCAM1 is associated with neurodegenerative conditions like Alzheimer's disease and certain types of cancer including neuroblastoma. Changes in NCAM1 expression or function can disrupt normal cell adhesion and signaling leading to pathological states. The protein's interaction with cell surface markers such as CD56 mouse marker helps in the diagnosis and therapy of these disorders providing potential therapeutic targets or biomarkers for clinical applications.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Flow Cytometry - Alexa Fluor® 594 Anti-NCAM1 antibody [EPR26939-108] (ab319185), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 594 Anti-NCAM1 antibody [EPR26939-108] (ab319185)

    Flow cytometric analysis of MCF7 (human breast adenocarcinoma epithelial cell, Left) / No-GFP-CD16.NK-92 (CD16 ectopically expressed natural killer(NK-92®) cell with no GFP tag, Right) cells labelling NCAM1 with ab319185 at 1/50 dilution (0.1ug) (Magenta) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab208568) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Negative control: MCF7 (PMID: 30050775).

    Gated on viable cells.

  • Flow Cytometry - Alexa Fluor® 594 Anti-NCAM1 antibody [EPR26939-108] (ab319185), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 594 Anti-NCAM1 antibody [EPR26939-108] (ab319185)

    Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling NCAM1 with ab319185 at 1/50 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab208568) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Cells are co-stained with CD3 conjugated to Alexa Fluor®647.

    Gated on viable cells.

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-NCAM1 antibody [EPR26939-108] (ab319185), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-NCAM1 antibody [EPR26939-108] (ab319185)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized No-GFP-CD16.NK-92 (CD16 ectopically expressed natural killer(NK-92®) cell with no GFP tag) cells labelling NCAM1 with ab319185 at 1/50 (10.0 ug/ml) dilution (Green).

    Confocal image showing membranous staining in No-GFP-CD16.NK-92 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).

    Negative control: MCF7 (PMID:30050775).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com