AB198516

Alexa Fluor® 594 Anti-nNOS (neuronal) antibody [EP1855Y]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal nNOS antibody - conjugated to Alexa Fluor® 594. Suitable for ICC/IF and reacts with Rat samples. Cited in 1 publication.

View Alternative Names

Nitric oxide synthase 1, Constitutive NOS, NC-NOS, NOS type I, Neuronal NOS, Peptidyl-cysteine S-nitrosylase NOS1, N-NOS, nNOS, bNOS, NOS1

1 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-nNOS (neuronal) antibody [EP1855Y] (AB198516)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-nNOS (neuronal) antibody [EP1855Y] (AB198516)

ab198516 staining nNOS in PC12 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198516 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at 2μg/ml (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Unconjugated

Anti-nNOS (neuronal) antibody [EP1855Y]
Carrier free

Anti-nNOS (neuronal) antibody [EP1855Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1855Y

Isotype

IgG

Conjugation

Alexa Fluor® 594

Excitation/Emission

Ex: 590nm, Em: 617nm

Carrier free

Reacts with

Rat

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NNOS also known as nitric oxide synthase 1 or NOS1 is a critical enzyme in the nervous system. It catalyzes the production of nitric oxide from L-arginine. This enzyme weighing approximately 160 kDa is expressed in neurons and you can find it in regions like the brain and spinal cord. Notably nNOS is part of the NOS family which also includes eNOS and iNOS but its primary function is in neuronal tissues.

Biological function summary

NNOS plays a significant role in neurotransmission and neurovascular regulation. As a part of protein complexes nNOS closely interacts with other signaling molecules to modulate neuronal communication. The production of nitric oxide by nNOS acts as a neurotransmitter influencing brain functions such as synaptic plasticity and memory formation. Additionally due to its role in neuronal activity antibodies like anti-neuronal antibodies can have interactions that can be studied using various assays including the NOS assay.

Pathways

NNOS serves essential roles within the neuronal nitric oxide signaling pathway and the glutamatergic signaling pathway. In these contexts nNOS associates with proteins involved in cell signaling such as calmodulin which regulates its activity. By participating in these pathways nNOS contributes to the regulation of vascular tone and synaptic transmission linking it to neurological processes and supporting neuronal communication.

NNOS shows significant connections to neurological disorders and cardiovascular diseases. In conditions like stroke and neurodegenerative diseases dysregulation of nNOS activity leads to excessive production of nitric oxide which can result in oxidative stress and neuronal damage. Furthermore its relation with eNOS in the cardiovascular system influences vascular function making it a critical player in hypertension studies. Understanding nNOS interactions through diseases highlights the possibility of targeted treatments using neuronal antibodies and anti-neuronal antibodies against specific pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.

See full target information Nitric oxide synthase, brain

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Respiratory research 25:365 PubMed39385167

2024

Exploring the diagnostic and immune infiltration roles of disulfidptosis related genes in pulmonary hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Tan,Ningning Zhang,Ge Zhang,Shuai Xu,Yiyao Zeng,Fenlan Bian,Bi Tang,Hongju Wang,Jili Fan,Xiaohong Bo,Yangjun Fu,Huimin Fan,Yafeng Zhou,Pinfang Kang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 594 Anti-nNOS (neuronal) antibody [EP1855Y]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-nNOS (neuronal) antibody [EP1855Y] (AB198516)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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