AB211838

Alexa Fluor® 594 Anti-PDHB antibody [EPR11097(B)]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal PDHB antibody - conjugated to Alexa Fluor® 594. Suitable for ICC/IF and reacts with Human samples. Cited in 2 publications.

View Alternative Names

PHE1B, PDHB, PDHE1-B

1 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-PDHB antibody [EPR11097(B)] (AB211838)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-PDHB antibody [EPR11097(B)] (AB211838)

ab211838 staining PDHB in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).

665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-PDHB antibody [EPR11097(B)]
421 Alexa Fluor® 405

Alexa Fluor® 405 Anti-PDHB antibody [EPR11097(B)]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-PDHB antibody [EPR11097(B)]
578 PE

PE Anti-Pyruvate Dehydrogenase E1 beta subunit antibody [EPR11097(B)]
660 APC

APC Anti-Pyruvate Dehydrogenase E1 beta subunit antibody [EPR11097(B)]
HRP

HRP Anti-Pyruvate Dehydrogenase E1 beta subunit antibody [EPR11097(B)]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Pyruvate Dehydrogenase E1 beta subunit antibody [EPR11097(B)]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-PDHB antibody [EPR11097(B)]
Unconjugated

Anti-PDHB antibody [EPR11097(B)]
Carrier free

Anti-PDHB antibody [EPR11097(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11097(B)

Isotype

IgG

Conjugation

Alexa Fluor® 594

Excitation/Emission

Ex: 590nm, Em: 617nm

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p>This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).</p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDHB also known as pyruvate dehydrogenase beta subunit plays an important role in the conversion of pyruvate to acetyl-CoA. This protein forms a part of the pyruvate dehydrogenase complex (PDC) which occurs in mitochondria of numerous tissues. The PDHB subunit has a molecular mass of approximately 39 kDa. Expression of PDHB is not limited to one specific type of tissue it is generally found in various tissues indicating its widespread involvement in cellular metabolism.

Biological function summary

PDHB functions as an essential component of the PDC which catalyzes the decarboxylation of pyruvate linking glycolysis to the citric acid cycle. The PDC itself is a large multi-enzyme complex including other subunits such as E1 and E2 which coordinate efficiently to carry out its function. In this way PDHB contributes to energy production by enabling entry of carbon skeletons into the tricarboxylic acid cycle important for ATP generation.

Pathways

PDHB holds a significant role in central metabolic pathways namely glycolysis and the citric acid cycle. The conversion facilitated by the PDC forms a vital cornerstone for metabolic flux regulation influencing gluconeogenesis and fatty acid synthesis. PDHB also interacts with proteins such as pyruvate dehydrogenase kinase which regulates the PDC activity by phosphorylation thereby affecting energy metabolism directly.

Alterations in PDHB can lead to metabolic disorders such as pyruvate dehydrogenase deficiency potentially causing neurological dysfunction due to impaired energy metabolism. PDHB mutations also have a link to mitochondrial diseases reflecting its significant role in energy homeostasis. In these contexts proteins like the pyruvate dehydrogenase phosphatase are involved highlighting the clinical relevance of PDHB alterations in maintaining proper metabolic health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.

See full target information PDHB

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature metabolism 4:589-607 PubMed35618940

2022

PARK7/DJ-1 promotes pyruvate dehydrogenase activity and maintains T homeostasis during ageing.

Applications

Unspecified application

Species

Unspecified reactive species

Egle Danileviciute,Ni Zeng,Christophe M Capelle,Nicole Paczia,Mark A Gillespie,Henry Kurniawan,Mohaned Benzarti,Myriam P Merz,Djalil Coowar,Sabrina Fritah,Daniela Maria Vogt Weisenhorn,Gemma Gomez Giro,Melanie Grusdat,Alexandre Baron,Coralie Guerin,Davide G Franchina,Cathy Léonard,Olivia Domingues,Sylvie Delhalle,Wolfgang Wurst,Jonathan D Turner,Jens Christian Schwamborn,Johannes Meiser,Rejko Krüger,Jeff Ranish,Dirk Brenner,Carole L Linster,Rudi Balling,Markus Ollert,Feng Q Hefeng

Biomaterials 198:180-193 PubMed29548546

2018

The effects of monocytes on tumor cell extravasation in a 3D vascularized microfluidic model.

Applications

Unspecified application

Species

Unspecified reactive species

A Boussommier-Calleja,Y Atiyas,K Haase,M Headley,C Lewis,R D Kamm

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 594 Anti-PDHB antibody [EPR11097(B)]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-PDHB antibody [EPR11097(B)] (AB211838)

Related conjugates and formulations (10)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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