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Rabbit Recombinant Monoclonal PRAME antibody - conjugated to Alexa Fluor® 594. Suitable for Flow Cyt (Intra) and reacts with Human samples.

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Images

Flow Cytometry - Alexa Fluor® 594 Anti-PRAME antibody [EPR20330] (AB312896), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
Alexa Fluor® 594
Excitation/Emission
Ex: 590nm, Em: 617nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)IHC-PICC/IF
Human
Tested
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/5000
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Version

Target data

Function

The protein expressed by gene PRAME functions as a transcriptional repressor, inhibiting retinoic acid signaling through retinoic acid receptors RARA, RARB, and RARG. It prevents retinoic acid-induced cell proliferation arrest, differentiation, and apoptosis. This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

MAPE, OIP4, PRAME, Melanoma antigen preferentially expressed in tumors, Opa-interacting protein 4, Preferentially expressed antigen of melanoma, OIP-4

Recommended products

Rabbit Recombinant Monoclonal PRAME antibody - conjugated to Alexa Fluor® 594. Suitable for Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype
IgG
Conjugation
Alexa Fluor® 594
Excitation/Emission
Ex: 590nm, Em: 617nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR20330
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Flow Cytometry - Alexa Fluor® 594 Anti-PRAME antibody [EPR20330] (ab312896), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 594 Anti-PRAME antibody [EPR20330] (ab312896)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Ramos (human Burkitt's lymphoma B lymphocyte, Left). K562 (human chronic myelogenous leukemia lymphoblast, Right) cells labelling PRAME with ab312896 at 1/5000 dilution (0.01 ug)/Red (Red) compared with a Rabbit monoclonal IgG (Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab208568) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Negative control: Ramos (PMID:9047241).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com