Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control

40 product images

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208568)

  Immunofluorescent analysis of HeLa (human cervical cancer) cells, fixed with 4% formaldehyde (10 min). The cells were permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab208568 (Rabbit IgG, monoclonal [EPR25A] - Isotype Control) at 1/100 dilution (showing no signal) and Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
  

  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).

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  Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208568)

  Flow Cytometry staining of Mouse PBMCs (mouse primary peripheral blood mononuclear cells) using rabbit Anti- antibody

  Flow cytometric analysis of Mouse PBMCs (mouse primary peripheral blood mononuclear cells) cells labelling CD27 with Alexa Fluor® 594 Anti-CD27 antibody [EPR27428-9] ab324177 at 1/50 dilution (1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) / Left isotype control.

  Cells were co-stained with anti CD3 conjugated to Alexa Fluor®647.
  
  Gated on viable cells.

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  Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208568)

  Flow Cytometry staining of Human PBMC (human peripheral blood mononuclear cell) using rabbit Anti- antibody

  Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD27 with Alexa Fluor® 594 Anti-CD27 antibody [EPR27428-9] ab324177 at 1/50 dilution (1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) / Left isotype control.

  Cells were co-stained with anti CD11b conjugated to Brilliant Violet 421.
  
  Gated on viable cells.

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  Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208568)

  Flow Cytometry staining of Human PBMC (human peripheral blood mononuclear cell) using rabbit Anti- antibody

  Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD27 with Alexa Fluor® 594 Anti-CD27 antibody [EPR27428-9] ab324177 at 1/50 dilution (1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) / Left isotype control.

  Cells were co-stained with anti CD3 conjugated to Alexa Fluor®647.
  
  Gated on viable cells.

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  Flow Cytometry (Intracellular) - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208568)

  Flow Cytometry (Intracellular) staining using rabbit Anti- antibody

  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Isotype (Left) / 293T cells transfected with a mouse CD27 expression vector (Middle) / 293T cells transfected with an empty expression vector (Right) cells labelling CD27 with Alexa Fluor® 594 Anti-CD27 antibody [EPR27428-9] ab324177 at 1/5000 dilution (0.01ug) / Middle and Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) / Left isotype control.

  Cells were co-stained with Myc tag conjugated to Alexa Fluor®647.

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  Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208568)

  Flow Cytometry staining using rabbit Anti- antibody

  Flow cytometric analysis of EL4 (mouse lymphoma T lymphocyte, Left) / J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage, Right) cells labelling CD13 with Alexa Fluor® 594 Anti-CD13 antibody [EPR27472-288] ab323786 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Gated on viable cells.
  
  Negative control: EL4 (PMID: 8103749).

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  Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208568)

  Flow Cytometry staining of Mouse spleen cell using rabbit Anti- antibody

  Flow cytometric analysis of Mouse spleen cell cells labelling CD13 with Alexa Fluor® 594 Anti-CD13 antibody [EPR27472-288] ab323786 at 1/50 dilution (1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Cells were co-stained with anti-CD3 conjugated to Alexa Fluor®647.
  
  Gated on viable cells.

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  Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208568)

  Flow Cytometry staining of Mouse spleen cell using rabbit Anti- antibody

  Flow cytometric analysis of Mouse spleen cell cells labelling CD13 with Alexa Fluor® 594 Anti-CD13 antibody [EPR27472-288] ab323786 at 1/50 dilution (1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Cells were co-stained with anti-CD11c conjugated to Brilliant Violet 421.
  
  Gated on viable cells.

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  Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208568)

  Flow Cytometry staining of Mouse peripheral blood mononuclear cell (PBMC) using rabbit Anti- antibody

  Flow cytometric analysis of Mouse peripheral blood mononuclear cell (PBMC) cells labelling CD45 with Alexa Fluor® 594 Anti-CD45 antibody [EPR28934-536] ab323889 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Gated on viable cells.

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  Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208568)

  Flow Cytometry staining using rabbit Anti- antibody

  Flow cytometric analysis of HL-1 (mouse atrial muscle cell, Left) / J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage, Right) cells labelling CD45 with Alexa Fluor® 594 Anti-CD45 antibody [EPR28934-536] ab323889 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Low expression: HL-1.
  
  Gated on viable cells.

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  Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208568)

  Flow Cytometry staining using rabbit Anti- antibody

  Flow cytometric analysis of C2C12 (mouse myoblast, Left) / RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage, Right) cells labelling CD45 with Alexa Fluor® 594 Anti-CD45 antibody [EPR28934-536] ab323889 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Low expression: C2C12.
  
  Gated on viable cells.

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  Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208568)

  Flow Cytometry staining of Mouse PBMC (mouse peripheral blood mononuclear cell) using rabbit Anti- antibody

  Flow cytometric analysis of Mouse PBMC (mouse peripheral blood mononuclear cell) cells labelling CD36 with Alexa Fluor® 594 Anti-CD36 antibody [EPR27433-40] ab323888 at 1/50 dilution (1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Cells were co-stained with anti CD3 conjugated to Alexa Fluor®647.
  
  Gated on viable cells.

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  Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208568)

  Flow Cytometry staining of Mouse PBMC (mouse peripheral blood mononuclear cell) using rabbit Anti- antibody

  Flow cytometric analysis of Mouse PBMC (mouse peripheral blood mononuclear cell) cells labelling CD36 with Alexa Fluor® 594 Anti-CD36 antibody [EPR27433-40] ab323888 at 1/50 dilution (1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Cells were co-stained with anti CD11b conjugated to Brilliant Violet 421.
  
  Gated on viable cells.

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  Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208568)

  Flow Cytometry staining using rabbit Anti- antibody

  Flow cytometric analysis of NIH/3T3 (mouse embryonic fibroblast, Left) / RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage, Right) cells labelling CD36 with Alexa Fluor® 594 Anti-CD36 antibody [EPR27433-40] ab323888 at 1/50 dilution (1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Negative control: NIH/3T3 (PMID: 7543064).
  
  Gated on viable cells.

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  Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208568)

  Flow Cytometry staining of Mouse bone marrow using rabbit Anti- antibody

  Flow cytometric analysis of Mouse bone marrow cells labelling CCR3 with Alexa Fluor® 594 Anti-CCR3 antibody [EPR27419-30] ab323787 at 1/50 dilution (1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Cells were co-stained with anti-CD3 cojugated to Alexa Fluor®488.
  
  Gated on viable cells.

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  Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208568)

  Flow Cytometry staining of Mouse bone marrow using rabbit Anti- antibody

  Flow cytometric analysis of Mouse bone marrow cells labelling CCR3 with Alexa Fluor® 594 Anti-CCR3 antibody [EPR27419-30] ab323787 at 1/50 dilution (1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Cells were co-stained with anti-CD11b conjugated to Brilliant Violet 421.
  
  Gated on viable cells.

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  Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208568)

  Flow Cytometry staining using rabbit Anti- antibody

  Flow cytometric analysis of A20 (mouse reticulum sarcoma B lymphocyte, Left) / J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage, Right) cells labelling CCR3 with Alexa Fluor® 594 Anti-CCR3 antibody [EPR27419-30] ab323787 at 1/50 dilution (1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Gated on viable cells.
  
  Low expression: A20.

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  Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208568)
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  Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208568)

  Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CCR9 with Alexa Fluor® 594 Anti-CCR9 antibody [EPR26524-59] ab323442 at 1/50 dilution (1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Cells are co-stained with CD56 conjugated to BV421.
  
  Gated on viable cells.

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  Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208568)

  Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CCR9 with Alexa Fluor® 594 Anti-CCR9 antibody [EPR26524-59] ab323442 at 1/50 dilution (1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Cells are co-stained with CD4 conjugated to Alexa Fluor®647.
  
  Gated on viable cells.

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  Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208568)

  Flow cytometric analysis of Jurkat (human T cell leukemia T lymphocyte from peripheral blood, Left) / MOLT-4 (human lymphoblastic leukemia T lymphoblast, Right) cells labelling CCR9 with Alexa Fluor® 594 Anti-CCR9 antibody [EPR26524-59] ab323442 at 1/50 dilution (1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Negative control: Jurkat (PMID: 19525985).
  
  Gated on viable cells.

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  Flow Cytometry (Intracellular) - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208568)

  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling Transaldolase 1 with Alexa Fluor® 594 Anti-Transaldolase 1 antibody [EPR28140-51] ab323464 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

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  Flow Cytometry (Intracellular) - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208568)

  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized A431 (human epidermoid carcinoma epithelial cell) cells labelling Transaldolase 1 with Alexa Fluor® 594 Anti-Transaldolase 1 antibody [EPR28140-51] ab323464 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

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  Flow Cytometry (Intracellular) - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208568)

  Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Mouse splenocytes treated with cell stimulation cocktail (80nM PMA+1.34uM Ionomycin+10.6uM Brefeldin A+2uM Monensin) for 6 hours (Lower) / Untreated control (Upper). cells labelling IL-2 with Alexa Fluor® 594 Anti-IL-2 antibody [EPR16615-341] ab323260 at 1/50 dilution (1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor? 594) (ab208568) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Cells were surface stained with anti-CD3 conjugated to Alexa Fluor?647. Then fixed with 2% PFA for 10min followed by intracellularly stained with antibody.
  
  Gated on lymphocytes population.

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  Flow Cytometry (Intracellular) - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208568)

  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized EL4.IL-2 (mouse lymphoma T lymphocyte) treated with cell stimulation cocktail (80nM PMA + 1.34uM Ionomycin + 10.6uM Brefeldin A + 2uM Monensin) for 6 hours (Green) / Untreated control (Magenta). cells labelling IL-2 with Alexa Fluor® 594 Anti-IL-2 antibody [EPR16615-341] ab323260 at 1/50 dilution (1ug) (Magenta) and Green compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor? 594) (ab208568) (Black) and Grey isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

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  Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208568)

  Flow cytometric analysis of Daudi (human Burkitt's lymphoma lymphoblast, Left) / KG-1a (human bone marrow myeloblast, Right) cells labelling CD34 with Alexa Fluor® 594 Anti-CD34 antibody [EPR27432-54] ab322731 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

  Negative control: Daudi (PMID: 21286385, 10942240).
  
  Gated on viable cells.

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  Flow Cytometry (Intracellular) - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208568)

  Flow cytometry overlay histogram showing left Jurkat positive cells, and right, negative HEK293T cells stained with Alexa Fluor® 594 Anti-CD45 antibody [EP322Y] ab303598 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (Alexa Fluor® 594 Anti-CD45 antibody [EP322Y] ab303598) (1x 106 in 100μl at 0.04μg/ml (1/12500)) for 30 min at 22°C.
  
  Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor® 594 (ab208568) used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue line) was also used as a control.
  
  Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561 nm) and 610/20 bandpass filter.
  
  This antibody gave a positive signal in Jurkat cells fixed with 4% formaldehyde (10 min) / permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

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  Flow Cytometry (Intracellular) - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208568)

  Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with Alexa Fluor® 594 Anti-CD45 antibody [EP322Y] ab303598 (right) or Rabbit IgG (monoclonal) Alexa Fluor® 594 (ab208568) isotype (left). Cells were fixed and permeabilized with BD Cytofix/Cytoperm™ for 20 min. PBMCs were incubated for 30 min on ice in 1x PBS containing 10μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (Alexa Fluor® 594 Anti-CD45 antibody [EP322Y] ab303598) or Rabbit IgG (monoclonal) Alexa Fluor® 594 (ab208568) isotype (100 μl at 0.2μg/ml (1/2500)) for 30 min on ice.
  
  Acquisition of >30000 events were collected using a 50 mW Yellow/Green laser (561 nm) and 610/20 bandpass filter. Events were gated on live cells.

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  Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208568)

  Flow cytometric analysis of K-562 (human chronic myelogenous leukemia lymphoblast, Left) / Raji (human Burkitt's lymphoma B lymphocyte, Right) cells labelling CD21 with Alexa Fluor® 594 Anti-CD21 antibody [EPR27369-9] ab322130 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Negative control: K-562 (PMID: 6983911).
  
  Gated on viable cells.

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  Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208568)

  Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD2 with Alexa Fluor® 594 Anti-CD2 antibody [EPR27426-17] ab319188 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Cells are co-stained with CD14 conjugated to FITC.
  
  Gated on viable cells.

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  Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208568)

  Flow cytometric analysis of MCF7 (human breast adenocarcinoma epithelial cell, Left) / No-GFP-CD16.NK-92 (CD16 ectopically expressed natural killer(NK-92®) cell with no GFP tag, Right) cells labelling NCAM1 with Alexa Fluor® 594 Anti-NCAM1 antibody [EPR26939-108] ab319185 at 1/50 dilution (0.1ug) (Magenta) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Negative control: MCF7 (PMID: 30050775).
  
  Gated on viable cells.

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  Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208568)

  Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling NCAM1 with Alexa Fluor® 594 Anti-NCAM1 antibody [EPR26939-108] ab319185 at 1/50 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Cells are co-stained with CD3 conjugated to Alexa Fluor®647.
  
  Gated on viable cells.

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  Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208568)

  Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD2 with Alexa Fluor® 594 Anti-CD2 antibody [EPR27426-17] ab319188 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Cells are co-stained with CD56 conjugated to Brilliant Violet 421.
  
  Gated on viable cells.

• 

  Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208568)

  Flow cytometric analysis of Raji (human Burkitt's lymphoma B lymphocyte, Left) / Jurkat (human T cell leukemia T lymphocyte from peripheral blood, Right) cells labelling CD2 with Alexa Fluor® 594 Anti-CD2 antibody [EPR27426-17] ab319188 at 1/500 dilution (0.1ug) (Magenta) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Negative control: Raji (PMID: 32808354).
  
  Gated on viable cells.

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  Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208568)

  Flow cytometric analysis of K-562 (human chronic myelogenous leukemia lymphoblast, Left) / U-2 OS (human bone osteosarcoma epithelial cell, Right) cells labelling CD90 / Thy1 with Alexa Fluor® 594 Anti-CD90 / Thy1 antibody [EPR28145-53] ab317533 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

  Gated on viable cells.Negative control: K-562.

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  Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208568)

  Flow cytometric analysis of PC-12 (rat adrenal gland pheochromocytoma) cells labelling CD90 / Thy1 with Alexa Fluor® 594 Anti-CD90 / Thy1 antibody [EPR28145-53] ab317533 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

  Gated on viable cells.

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  Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208568)

  Flow cytometric analysis of Mouse peripheral blood mononuclear cell (PBMC) cells labelling CD90 / Thy1 with Alexa Fluor® 594 Anti-CD90 / Thy1 antibody [EPR28145-53] ab317533 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

  Cells were stained with anti-CD3 conjugated to Alexa Fluor® 488.Gated on viable cells.

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  Flow Cytometry (Intracellular) - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208568)

  Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Isotype (Left) / Human PBMC (human peripheral blood mononuclear cell) treated with cell stimulation cocktail for 6 hours (Middle) / Untreated control (Right) cells labelling Interferon gamma with Alexa Fluor® 594 Anti-Interferon gamma antibody [EPR23991-53] ab317059 at 1/500 dilution (0.1ug)/ Middle and Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) / Left isotype control.

  Cells were stained with anti-CD4 conjugated to Alexa Fluor® 647. Then fixed with 2% PFA for 10min followed by intracellularly stained with rabbit IgG or Alexa Fluor® 594 Anti-Interferon gamma antibody [EPR23991-53] ab317059.

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  Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208568)

  Flow cytometric analysis of Mouse splenocytes cells labelling Klra8 with Alexa Fluor® 594 Anti-Klra8 antibody [EPR28281-49] ab316879 at 1/500 dilution (0.1 ug)/Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) / Left isotype control.

  Mouse splenocytes are co-stained with NK1.1 conjugated Alexa Fluor®647. Gated on viable cell.

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  Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208568)

  Flow cytometric analysis of Mouse splenocytes cells labelling Klra8 with Alexa Fluor® 594 Anti-Klra8 antibody [EPR28281-49] ab316879 at 1/500 dilution (0.1 ug)/Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) / Left isotype control.

  Mouse splenocytes are co-stained with CD3 conjugated Alexa Fluor®488. Gated on viable cell.