AB208568

Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control

RabMAb
Recombinant
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Anti-Rabbit IgG, monoclonal [EPR25A] - Isotype Control- Alexa Fluor® 594 conjugated (ab208568) is a rabbit recombinant monoclonal antibody used in Flow Cytometry, ICC/IF.

- Biophysical QC for unrivalled batch-batch consistency

40 Images

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow cytometric analysis of Human PBMC(human peripheral blood mononuclear cell) cells labelling CD45 with ab315959 at 1/500 dilution (0.1ug)/Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) / Left isotype control.

Human PBMCs are co-stained with CD3 conjugated Alexa Fluor®647. Gated on viable cell.

Flow Cytometry (Intracellular) - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Isotype (Left) / 293T cells transfected with a mouse CD27 expression vector (Middle) / 293T cells transfected with an empty expression vector (Right) cells labelling CD27 with ab324177 at 1/5000 dilution (0.01ug) / Middle and Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) / Left isotype control.

Cells were co-stained with Myc tag conjugated to Alexa Fluor®647.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Human PBMC(human peripheral blood mononuclear cell) cells labelling T-bet / Tbx21 with ab315425 at 1/500 dilution (0.1ug)/Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) / Left isotype control.

Human PBMC are co-stained with CD56 conjugated to BV421.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab303598 (right) or Rabbit IgG (monoclonal) Alexa Fluor® 594 (ab208568) isotype (left). Cells were fixed and permeabilized with BD Cytofix/Cytoperm™ for 20 min. PBMCs were incubated for 30 min on ice in 1x PBS containing 10μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab303598) or Rabbit IgG (monoclonal) Alexa Fluor® 594 (ab208568) isotype (100 μl at 0.2μg/ml (1/2500)) for 30 min on ice.

Acquisition of >30000 events were collected using a 50 mW Yellow/Green laser (561 nm) and 610/20 bandpass filter. Events were gated on live cells.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow cytometry overlay histogram showing left Jurkat positive cells, and right, negative HEK293T cells stained with ab303598 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab303598) (1x 106 in 100μl at 0.04μg/ml (1/12500)) for 30 min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor® 594 (ab208568) used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561 nm) and 610/20 bandpass filter.

This antibody gave a positive signal in Jurkat cells fixed with 4% formaldehyde (10 min) / permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Isotype (Left) / Human PBMC (human peripheral blood mononuclear cell) treated with cell stimulation cocktail for 6 hours (Middle) / Untreated control (Right) cells labelling Interferon gamma with ab317059 at 1/500 dilution (0.1ug)/ Middle and Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) / Left isotype control.

Cells were stained with anti-CD4 conjugated to Alexa Fluor® 647. Then fixed with 2% PFA for 10min followed by intracellularly stained with rabbit IgG or ab317059.

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow cytometric analysis of 293T (human embryonic kidney epithelial cell, Left) / Jurkat (human T cell leukemia T lymphocyte from peripheral blood, Right) cells labelling CD45 with ab315959 at 1/500 dilution (0.1 ug)/Red compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Negative control : 293T. Gated on viable cells.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa(human cervical adenocarcinoma epithelial cell) cells labelling Pericentrin with ab314881 at 1/50 dilution (1 ug)/Red (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow cytometric analysis of L1CAM KO HeLa (human cervical adenocarcinoma epithelial cell, Left)

Parental HeLa(Right) cells labelling L1CAM with ab315360 at 1/500 dilution (0.1 ug)/Red compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Gated on viable cell.

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cell) cells labelling CD11a with ab314469 at 1/500 dilution (0.1 ug)/Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) / Left isotype control and an unlabelled control (cells without incubation with primary antibody (Blue). Cells are co-stained with CD3 conjugated to Alexa Fluor®647. Gated on viable cells.

Flow Cyt

Lab

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow Cyt

Lab

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow cytometric analysis of HeLa (human cervix adenocarcinoma epithelial cell, left) cells and THP-1 (human monocytic leukemia monocyte, right) cells labeling CD11a with ab314469 at 1/500 (red) compared with Rabbit IgG, monoclonal [EPR25A] - Isotype Control Alexa Fluor^® 594 (ab208568) (black).

Blocking : 10% Goat serum.

Gated on viable cells.

Negative control : Hela (PMID : 12036881).

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Human PBMC are co-stained with CD19 conjugated to Alexa Fluor®647, showing a distinct CD19 positive population with no cross reactivity to TBX21 positive cells.

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD18 with ab313752 at 1/500 dilution (0.1 ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Cells co-stained with CD14 conjugated to A647. Gated on viable cells.

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling CD14 with ab305119 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Gated on viable cells.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD27 with ab324177 at 1/50 dilution (1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) / Left isotype control.

Cells were co-stained with anti CD11b conjugated to Brilliant Violet 421.
Gated on viable cells.

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Cells were co-stained with anti CD3 conjugated to Alexa Fluor®647.
Gated on viable cells.

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CCR9 with ab323442 at 1/50 dilution (1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Cells are co-stained with CD4 conjugated to Alexa Fluor®647.
Gated on viable cells.

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow cytometric analysis of Jurkat (human T cell leukemia T lymphocyte from peripheral blood, Left) / MOLT-4 (human lymphoblastic leukemia T lymphoblast, Right) cells labelling CCR9 with ab323442 at 1/50 dilution (1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Negative control : Jurkat (PMID : 19525985).
Gated on viable cells.

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Cells are co-stained with CD56 conjugated to BV421.
Gated on viable cells.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized A431 (human epidermoid carcinoma epithelial cell) cells labelling Transaldolase 1 with ab323464 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow cytometric analysis of K-562 (human chronic myelogenous leukemia lymphoblast, Left) / Raji (human Burkitt's lymphoma B lymphocyte, Right) cells labelling CD21 with ab322130 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Negative control : K-562 (PMID : 6983911).
Gated on viable cells.

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow cytometric analysis of Daudi (human Burkitt's lymphoma lymphoblast, Left) / KG-1a (human bone marrow myeloblast, Right) cells labelling CD34 with ab322731 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Negative control : Daudi (PMID : 21286385, 10942240).
Gated on viable cells.

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow cytometric analysis of K-562 (human chronic myelogenous leukemia lymphoblast, Left) / U-2 OS (human bone osteosarcoma epithelial cell, Right) cells labelling CD90 / Thy1 with ab317533 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Gated on viable cells.Negative control : K-562.

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow cytometric analysis of Raji (human Burkitt's lymphoma B lymphocyte, Left) / Jurkat (human T cell leukemia T lymphocyte from peripheral blood, Right) cells labelling CD2 with ab319188 at 1/500 dilution (0.1ug) (Magenta) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Negative control : Raji (PMID : 32808354).
Gated on viable cells.

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD2 with ab319188 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Cells are co-stained with CD56 conjugated to Brilliant Violet 421.
Gated on viable cells.

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling NCAM1 with ab319185 at 1/50 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Cells are co-stained with CD3 conjugated to Alexa Fluor®647.
Gated on viable cells.

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Cells are co-stained with CD14 conjugated to FITC.
Gated on viable cells.

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow cytometric analysis of MCF7 (human breast adenocarcinoma epithelial cell, Left) / No-GFP-CD16.NK-92 (CD16 ectopically expressed natural killer(NK-92®) cell with no GFP tag, Right) cells labelling NCAM1 with ab319185 at 1/50 dilution (0.1ug) (Magenta) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Negative control : MCF7 (PMID : 30050775).
Gated on viable cells.

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow cytometric analysis of Neuro-2a (mouse neuroblastoma neuroblast, Left) / NIH/3T3 (mouse embryonic fibroblast, Right) cells labelling CD34 with ab324151 at 1/50 dilution (1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Negative control : Neuro-2a.
Gated on viable cells.

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow cytometric analysis of L929 (mouse connective tissue fibroblast, Left) / Neuro-2a (mouse neuroblastoma neuroblast, Right) cells labeling NCAM1 with ab303720 at 1/500 dilution (0.1µg) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) (Black) and an unlabelled control (cells without incubation with primary antibody) (Blue). Negative control : L-929 (PMID : 9696812). Gated on viable cells.

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow cytometric analysis of mouse thymocytes cells labelling CD147 with ab303721 at 1/50 dilution (1µg)/ Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) / Left. Gated on viable cells.

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow cytometric analysis of Mouse peripheral blood mononuclear cell (PBMC) cells labelling CD90 / Thy1 with ab317533 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Cells were stained with anti-CD3 conjugated to Alexa Fluor® 488.Gated on viable cells.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Mouse splenocytes treated with cell stimulation cocktail (80nM PMA+1.34uM Ionomycin+10.6uM Brefeldin A+2uM Monensin) for 6 hours (Lower) / Untreated control (Upper). cells labelling IL-2 with ab323260 at 1/50 dilution (1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor^® 594) (ab208568) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Cells were surface stained with anti-CD3 conjugated to Alexa Fluor^® 647. Then fixed with 2% PFA for 10min followed by intracellularly stained with antibody.
Gated on lymphocytes population.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized EL4.IL-2 (mouse lymphoma T lymphocyte) treated with cell stimulation cocktail (80nM PMA + 1.34uM Ionomycin + 10.6uM Brefeldin A + 2uM Monensin) for 6 hours (Green) / Untreated control (Magenta). cells labelling IL-2 with ab323260 at 1/50 dilution (1ug) (Magenta) and Green compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor^® 594) (ab208568) (Black) and Grey isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow cytometric analysis of HL-1 (mouse atrial muscle cell, Left) / J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage, Right) cells labelling CD45 with ab323889 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Low expression : HL-1.
Gated on viable cells.

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow cytometric analysis of NIH/3T3 (mouse embryonic fibroblast, Left) / RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage, Right) cells labelling CD36 with ab323888 at 1/50 dilution (1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Negative control : NIH/3T3 (PMID : 7543064).
Gated on viable cells.

519 FITC

FITC Rabbit IgG, monoclonal [EPR25A] - Isotype Control
660 APC

APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control
675 PerCP

PerCP Rabbit IgG, monoclonal [EPR25A] - Isotype Control
578 PE

PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control
603 Alexa Fluor® 568

Alexa Fluor® 568 Rabbit IgG, monoclonal [EPR25A] - Isotype Control
519 Alexa Fluor® 488

Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control
HRP

HRP Rabbit IgG, monoclonal [EPR25A] - Isotype Control
665 Alexa Fluor® 647

Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control
421 Alexa Fluor® 405

Alexa Fluor® 405 Rabbit IgG, monoclonal [EPR25A] - Isotype Control
565 Alexa Fluor® 555

Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control
Biotin

Biotin Isotype Control [EPR25A]
775 Alexa Fluor® 750

Alexa Fluor® 750 Rabbit IgG, monoclonal [EPR25A] - Isotype Control
Unconjugated

Rabbit IgG, monoclonal [EPR25A] - Isotype Control
Carrier free

Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free
Carrier free

Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25A

Isotype

IgG

Conjugation

Alexa Fluor® 594

Excitation/Emission

Ex: 590nm, Em: 617nm

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P, Flow Cyt, Flow Cyt (Intra)

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "Please note: This product should be diluted to the same concentration (not dilution) of the primary antibody to be used.", "FlowCytIntra-species-checked": "", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

What is this antibody validated in?
Alexa Fluor^® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208568) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF).

Other related products
We have a range of other formats of antibody clone [EPR25A] also available for your convenience: ab172730, Alexa Fluor^® 488 - ab199091, Alexa Fluor^® 647 - ab199093, Carrier free - ab199376, HRP - ab199507, Alexa Fluor^® 405 - ab208150, Alexa Fluor^® 594 - ab208568, Alexa Fluor^® 555 - ab208569, PE - ab209478, Alexa Fluor^® 568 - ab209613, Carrier free - ab210849, PerCP - ab222107, FITC - ab223339, APC - ab232814, Biotin - ab320073, Alexa Fluor^® 750 - ab322086

Immunogen
Chemical / Small Molecule conjugated to keyhole limpet haemocyanin. KLH is a copper containing oxygen carrier occurring freely dissolved in the hemolymph of many molluscs and arthropods. KLH forms a large complex composed of ~50 kDa subunits.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature metabolism 4:589-607 PubMed35618940

2022

PARK7/DJ-1 promotes pyruvate dehydrogenase activity and maintains T homeostasis during ageing.

Applications

Unspecified application

Species

Unspecified reactive species

Egle Danileviciute,Ni Zeng,Christophe M Capelle,Nicole Paczia,Mark A Gillespie,Henry Kurniawan,Mohaned Benzarti,Myriam P Merz,Djalil Coowar,Sabrina Fritah,Daniela Maria Vogt Weisenhorn,Gemma Gomez Giro,Melanie Grusdat,Alexandre Baron,Coralie Guerin,Davide G Franchina,Cathy Léonard,Olivia Domingues,Sylvie Delhalle,Wolfgang Wurst,Jonathan D Turner,Jens Christian Schwamborn,Johannes Meiser,Rejko Krüger,Jeff Ranish,Dirk Brenner,Carole L Linster,Rudi Balling,Markus Ollert,Feng Q Hefeng

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Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow Cytometry (Intracellular) - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow Cytometry (Intracellular) - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow Cytometry (Intracellular) - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow Cytometry (Intracellular) - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow Cytometry (Intracellular) - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow Cytometry (Intracellular) - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow Cytometry (Intracellular) - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow Cytometry (Intracellular) - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow Cytometry (Intracellular) - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow Cytometry (Intracellular) - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow Cytometry (Intracellular) - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Flow Cytometry - Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208568)

Related conjugates and formulations (15)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions