Rabbit Recombinant Monoclonal RING2 / RING1B / RNF2 antibody - conjugated to Alexa Fluor® 594.
IgG
Rabbit
Alexa Fluor® 594
Ex: 590nm, Em: 617nm
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Monoclonal
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application Target Binding Affinity | Reactivity Expected | Dilution info - | Notes - |
Application Antibody Labelling | Reactivity Expected | Dilution info - | Notes - |
Select an associated product type
E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A (H2AK119Ub), thereby playing a central role in histone code and gene regulation (PubMed:15386022, PubMed:16359901, PubMed:21772249, PubMed:25355358, PubMed:25519132, PubMed:26151332, PubMed:33864376). H2AK119Ub gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. May be involved in the initiation of both imprinted and random X inactivation (By similarity). Essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development (PubMed:16359901, PubMed:26151332). PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility (PubMed:26151332). E3 ubiquitin-protein ligase activity is enhanced by BMI1/PCGF4 (PubMed:21772249). Acts as the main E3 ubiquitin ligase on histone H2A of the PRC1 complex, while RING1 may rather act as a modulator of RNF2/RING2 activity (Probable). Association with the chromosomal DNA is cell-cycle dependent. In resting B- and T-lymphocytes, interaction with AURKB leads to block its activity, thereby maintaining transcription in resting lymphocytes (By similarity). Also acts as a negative regulator of autophagy by mediating ubiquitination of AMBRA1, leading to its subsequent degradation (By similarity).
BAP1, DING, HIPI3, RING1B, HIPI3, RING1B, DING, BAP1, RNF2, E3 ubiquitin-protein ligase RING2, Huntingtin-interacting protein 2-interacting protein 3, Protein DinG, RING finger protein 1B, RING finger protein 2, RING finger protein BAP-1, RING-type E3 ubiquitin transferase RING2, HIP2-interacting protein 3, RING1b
Rabbit Recombinant Monoclonal RING2 / RING1B / RNF2 antibody - conjugated to Alexa Fluor® 594.
IgG
Rabbit
Alexa Fluor® 594
Ex: 590nm, Em: 617nm
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Monoclonal
EPR12245
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle, Store in the dark
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone. This conjugated antibody is eligible for the Abcam trial program.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This supplementary information is collated from multiple sources and compiled automatically.
RING2 also known as RING1B or RNF2 is a protein that functions as a member of the Polycomb Repressive Complex 1 (PRC1). This protein contains a RING-finger domain facilitating ubiquitin ligase activity. It has a molecular mass of approximately 38 kDa. RING2/RNF2/RING1B is expressed in various tissues including the brain heart and lungs indicating its fundamental role across different biological systems.
RING2/RNF2/RING1B regulates gene expression by modifying chromatin structure especially through monoubiquitination of histone H2A at lysine 119. This action occurs within the Polycomb Repressive Complex 1 a multiprotein structure important for maintaining the transcriptional repression of target genes. It influences cell cycle progression and development by silencing genes involved in proliferation and differentiation.
RING2/RNF2/RING1B's role connects to the Polycomb group (PcG) pathway and the Wnt signaling pathway. Within these pathways it interacts with proteins like BMI1 and EZH2 which are other components of polycomb complexes. These interactions help modulate chromatin dynamics and control the expression of several genes involved in cell identity and development.
Dysfunction or aberrant expression of RING2/RNF2/RING1B has links to cancer and neurological disorders. Its relationship with proteins like CBX8 ties it to pathways of oncogenesis where it can influence tumorigenesis through misregulation of gene silencing. Furthermore changes in RING2/RNF2/RING1B expression or function may contribute to developmental disorders associated with improper neuronal development.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com