Rabbit Recombinant Monoclonal RYBP antibody - conjugated to Alexa Fluor® 594.
IgG
Rabbit
Alexa Fluor® 594
Ex: 590nm, Em: 617nm
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Monoclonal
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Target Binding Affinity | Reactivity Expected | Dilution info - | Notes - |
Application Antibody Labelling | Reactivity Expected | Dilution info - | Notes - |
Select an associated product type
Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1-like complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed:25519132). Component of a PRC1-like complex that mediates monoubiquitination of histone H2A 'Lys-119' on the X chromosome and is required for normal silencing of one copy of the X chromosome in XX females. May stimulate ubiquitination of histone H2A 'Lys-119' by recruiting the complex to target sites (By similarity). Inhibits ubiquitination and subsequent degradation of TP53, and thereby plays a role in regulating transcription of TP53 target genes (PubMed:19098711). May also regulate the ubiquitin-mediated proteasomal degradation of other proteins like FANK1 to regulate apoptosis (PubMed:14765135, PubMed:27060496). May be implicated in the regulation of the transcription as a repressor of the transcriptional activity of E4TF1 (PubMed:11953439). May bind to DNA (By similarity). May play a role in the repression of tumor growth and metastasis in breast cancer by down-regulating SRRM3 (PubMed:27748911).
RING1 and YY1-binding protein, Apoptin-associating protein 1, Death effector domain-associated factor, YY1 and E4TF1-associated factor 1, APAP-1, DED-associated factor, YEAF1, DEDAF, RYBP
Rabbit Recombinant Monoclonal RYBP antibody - conjugated to Alexa Fluor® 594.
RING1 and YY1-binding protein, Apoptin-associating protein 1, Death effector domain-associated factor, YY1 and E4TF1-associated factor 1, APAP-1, DED-associated factor, YEAF1, DEDAF, RYBP
IgG
Rabbit
Alexa Fluor® 594
Ex: 590nm, Em: 617nm
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Monoclonal
EPR13059(2)
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle, Store in the dark
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone. This conjugated antibody is eligible for the Abcam trial program.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This supplementary information is collated from multiple sources and compiled automatically.
RYBP also known as Ring1 and YY1 Binding Protein has a critical mechanical role in gene regulation. It has a molecular weight of approximately 28 kDa. It is a part of the Polycomb group (PcG) proteins which take part in chromatin remodeling to regulate gene expression. RYBP is found in the nucleus of cells and is known to bind DNA directly. Its expression occurs widely across various tissues indicating its broad involvement in cellular functions.
This protein actively participates in maintaining transcriptional repression through its involvement in the Polycomb Repressive Complex 1 (PRC1). PRC1 includes other key proteins such as Ring1A/B and CBX proteins which work together to modulate chromatin structure. RYBP contributes to the ubiquitination of histone H2A a process essential for maintaining gene silencing. This activity is important during developmental stages influencing cell fate decisions and lineage commitment.
RYBP plays an essential role in the regulation of the Wnt signaling pathway and the apoptosis pathway. Within the Wnt pathway RYBP acts in part by interacting with proteins such as β-catenin influencing cell proliferation and differentiation. In the apoptosis pathway it is involved through its interactions with E3 ubiquitin ligases potentially affecting cell death regulation. These pathways showcase how RYBP integrates into important cellular processes impacting cellular homeostasis.
RYBP has connections to cancer and neurodevelopmental disorders. Its altered expression is linked to tumor progression where it interacts with other PcG proteins like EZH2 often showing overexpression in cancers. These interactions can lead to abnormal gene silencing contributing to oncogenesis. In neurodevelopmental contexts disruptions in RYBP's function are associated with changes in neural differentiation and might correlate with disorders like autism spectrum disorders highlighting its broader relevance beyond typical gene expression roles.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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