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Rabbit Monoclonal S100 beta antibody - conjugated to Alexa Fluor® 594. Suitable for IHC-Fr and reacts with Mouse, Rat samples.

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Images

Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (AB307851), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (AB307851), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

Alexa Fluor® 594

Excitation/Emission

Ex: 590nm, Em: 617nm

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-FrICC/IF
Mouse
Tested
Not recommended
Rat
Tested
Not recommended

Tested
Tested

Species

Mouse

Dilution info

1/50

Notes

-

Species

Rat

Dilution info

1/50

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

3 products for Alternative Version

    Target data

    Function

    Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity.

    Alternative names

    Protein S100-B, S-100 protein beta chain, S-100 protein subunit beta, S100 calcium-binding protein B, S100B

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    Rabbit Monoclonal S100 beta antibody - conjugated to Alexa Fluor® 594. Suitable for IHC-Fr and reacts with Mouse, Rat samples.

    Alternative names

    Protein S100-B, S-100 protein beta chain, S-100 protein subunit beta, S100 calcium-binding protein B, S100B

    Key facts

    Isotype

    IgG

    Conjugation

    Alexa Fluor® 594

    Excitation/Emission

    Ex: 590nm, Em: 617nm

    Form

    Liquid

    Clonality

    Monoclonal

    Immunogen
    • The exact immunogen used to generate this antibody is proprietary information.
    Clone number

    EP1576Y

    Purification technique

    Affinity purification Protein A

    Concentration
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    Storage

    Shipped at conditions

    Blue Ice

    Appropriate short-term storage duration

    1-2 weeks

    Appropriate short-term storage conditions

    +4°C

    Appropriate long-term storage conditions

    -20°C

    Aliquoting information

    Upon delivery aliquot

    Storage information

    Avoid freeze / thaw cycle, Store in the dark

    Notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free batch production

    For more information, read more on recombinant antibodies.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    Product promise

    We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

    In the unlikely event of one of our products not working as expected, you are covered by our product promise.

    Full details and terms and conditions can be found here:
    Terms & Conditions.

    2 product images

    • Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab307851), expandable thumbnail

      Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab307851)

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebrum tissue labeling S100 beta - Astrocyte Marker with ab307851 at 1/50 dilution (10.0 ug/ml).
      Confocal image showing positive staining on rat cerebrum.
      The nuclear counterstain was DAPI (Blue). The section was incubated with ab307851 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
      Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    • Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab307851), expandable thumbnail

      Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab307851)

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebrum tissue labeling S100 beta - Astrocyte Marker with ab307851 at 1/50 dilution (10.0 ug/ml).
      Confocal image showing positive staining on mouse cerebrum.
      The nuclear counterstain was DAPI (Blue). The section was incubated with ab307851 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
      Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

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    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

    Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

    For licensing inquiries, please contact partnerships@abcam.com