Alexa Fluor® 594 Anti-SDHA antibody [2E3GC12FB2AE2]

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-SDHA antibody [2E3GC12FB2AE2] (AB170172)

ab170172 staining SDHA in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab170172 at a 1/1000 dilution (shown in pseudo color red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-SDHA antibody [2E3GC12FB2AE2] (AB170172)

Immunocytochemistry with HDFn (100x) cells.
A) HDFn stained with anti-SDHA Alexa-594 antibody (1.0 μg/mL). B) HDFn stained with Anti-mitochondrial chaperone HSP60 (1/1000, ab46798), Secondary antibody used was goat anti-rabbit Alexa® 488 (1/1000, ab150077). C) DAPI as nuclear stain (1/10000). D) Merge of color channels to show specificity of signal to mitochondria.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-SDHA antibody [2E3GC12FB2AE2] (AB170172)

Immunocytochemistry with HeLa cells (100x).
A) HeLa stained with anti-SDHA Alexa-594 antibody (1.0 μg/mL). B) HeLa stained with Anti-HSP60 (1/1000, ab46798), Secondary antibody used was goat anti-rabbit Alexa® 488 (1/1000, ab150077). C) DAPI as nuclear stain (1/10000). D) Merge of color channels to show specificity of signal to mitochondria.