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Mouse Monoclonal SDHA antibody - conjugated to Alexa Fluor® 594. Suitable for ICC/IF and reacts with Human samples.

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Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-SDHA antibody [2E3GC12FB2AE2] (AB170172), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-SDHA antibody [2E3GC12FB2AE2] (AB170172), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-SDHA antibody [2E3GC12FB2AE2] (AB170172), expandable thumbnail

Key facts

Isotype
IgG1
Host species
Mouse
Conjugation
Alexa Fluor® 594
Excitation/Emission
Ex: 590nm, Em: 617nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IF
Human
Tested
Mouse
Predicted
Rat
Predicted
Cow
Predicted
Dog
Predicted

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

ab178000 - Mouse monoclonal IgG1 (Alexa Fluor® 594), is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species
Mouse, Rat, Cow, Dog
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Version

3 products for Alternative Product

Target data

Function

Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) (PubMed:10746566, PubMed:24781757). SDH also oxidizes malate to the non-canonical enol form of oxaloacetate, enol-oxaloacetate (By similarity). Enol-oxaloacetate, which is a potent inhibitor of the succinate dehydrogenase activity, is further isomerized into keto-oxaloacetate (By similarity). Can act as a tumor suppressor (PubMed:20484225).

Alternative names

SDH2, SDHF, SDHA, Flavoprotein subunit of complex II, Malate dehydrogenase [quinone] flavoprotein subunit, Fp

Recommended products

Mouse Monoclonal SDHA antibody - conjugated to Alexa Fluor® 594. Suitable for ICC/IF and reacts with Human samples.

Key facts

Isotype
IgG1
Conjugation
Alexa Fluor® 594
Excitation/Emission
Ex: 590nm, Em: 617nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
2E3GC12FB2AE2
Purity
IgG fraction
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

ab170172 was previously used as a component in the MitoBiogenesis™ ICC Kit. The protocol for this kit is available here.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product was previously marketed under the MitoSciences sub-brand.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Succinate dehydrogenase complex flavoprotein subunit A (SDHA) also known as complex II Fp or SDH2 plays an important role in the mitochondrial electron transport chain and the tricarboxylic acid (TCA) cycle. It functions as a flavoprotein oxidoreductase catalyzing the oxidation of succinate to fumarate. With a molecular mass of approximately 72 kDa SDHA is expressed in the inner mitochondrial membrane of eukaryotic cells where it is a core component of the succinate dehydrogenase complex (SDHC). The complex is essential for cellular respiration and energy production.

Biological function summary

SDHA participates in the TCA cycle by accepting electrons from succinate which it donates to the coenzyme Q in the electron transport chain. This essential role connects SDHA to the regulation of ATP production in cells. SDHA operates as part of the larger succinate dehydrogenase (SDH) complex which includes other subunits such as SDHB SDHC and SDHD. This structurally integrated multisubunit complex influences mitochondrial integrity and cellular energy homeostasis.

Pathways

SDHA is deeply involved in the TCA cycle and oxidative phosphorylation pathway. As a part of these pathways it links to other critical enzymes such as fumarase and aconitase working in concert to drive the conversion of biochemical fuel into usable cellular energy. Its interactions with coenzyme Q and cytochrome complex enzymes are important for electron flow and proton gradient formation across the mitochondrial membrane. Such interactions are central to cellular respiration and energy generation.

Associated diseases and disorders

Mutations in SDHA correlate with various mitochondrial diseases and cancer syndromes. Specifically SDHA mutations have an association with Leigh syndrome and certain types of mitochondrial complex II deficiency. These mutations disrupt the function of the SDH complex causing metabolic imbalances and energy production issues. Furthermore the integral interaction of SDHA with other SDH subunits means that alterations can impact this entire enzymatic complex with implications for cellular respiration and disease progression.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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3 product images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-SDHA antibody [2E3GC12FB2AE2] (ab170172), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-SDHA antibody [2E3GC12FB2AE2] (ab170172)

    ab170172 staining SDHA in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab170172 at a 1/1000 dilution (shown in pseudo color red) and Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-SDHA antibody [2E3GC12FB2AE2] (ab170172), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-SDHA antibody [2E3GC12FB2AE2] (ab170172)

    Immunocytochemistry with HDFn (100x) cells.
    A) HDFn stained with anti-SDHA Alexa-594 antibody (1.0 μg/mL). B) HDFn stained with Anti-mitochondrial chaperone HSP60 (1/1000, Anti-Hsp60 antibody ab46798), Secondary antibody used was goat anti-rabbit Alexa® 488 (1/1000, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077). C) DAPI as nuclear stain (1/10000). D) Merge of color channels to show specificity of signal to mitochondria.

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-SDHA antibody [2E3GC12FB2AE2] (ab170172), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-SDHA antibody [2E3GC12FB2AE2] (ab170172)

    Immunocytochemistry with HeLa cells (100x).
    A) HeLa stained with anti-SDHA Alexa-594 antibody (1.0 μg/mL). B) HeLa stained with Anti-HSP60 (1/1000, Anti-Hsp60 antibody ab46798), Secondary antibody used was goat anti-rabbit Alexa® 488 (1/1000, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077). C) DAPI as nuclear stain (1/10000). D) Merge of color channels to show specificity of signal to mitochondria.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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