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AB311668

Alexa Fluor® 594 Anti-SOX10 antibody [SP267]

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Rabbit Recombinant Monoclonal SOX10 antibody - conjugated to Alexa Fluor® 594. Suitable for ICC/IF, Flow Cyt and reacts with Human, Mouse samples.

View Alternative Names

Transcription factor SOX-10, SOX10

4 Images
Flow Cytometry - Alexa Fluor® 594 Anti-SOX10 antibody [SP267] (AB311668)
  • Flow Cyt

Lab

Flow Cytometry - Alexa Fluor® 594 Anti-SOX10 antibody [SP267] (AB311668)

Flow Cytometry analysis of HeLa (human cervix adenocarcinoma epithelial cell, Left) / A375 (human malignant melanoma epithelial cell, Right) labeling SOX10 with ab311668 at 1/500 dilution (0.1 μg)/Red. Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% methanol. A Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) (Black) is used as isotype control. Unlabelled control : without incubation with primary antibody and secondary antibody (Blue). Negative control : Hela.

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-SOX10 antibody [SP267] (AB311668)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-SOX10 antibody [SP267] (AB311668)

Immunocytochemistry/Immunofluorescence analysis of A375 (human malignant melanoma epithelial cell) labeling SOX10 with ab311668 at 1/50 (10.0 μg/mL). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% TritonX-100. ab195887 at 1/200 (2.5μg/mL) was as counterstain. Nuclear counterstain was DAPI. Confocal image showing nuclear staining in A375 cells. Negative control : HeLa (PMID : 29315345) Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Flow Cytometry - Alexa Fluor® 594 Anti-SOX10 antibody [SP267] (AB311668)
  • Flow Cyt

Lab

Flow Cytometry - Alexa Fluor® 594 Anti-SOX10 antibody [SP267] (AB311668)

Flow Cytometry analysis of B16-F0 (mouse skin melanoma cell) labeling SOX10 with ab311668 at 1/500 dilution (0.1 μg)/Red. Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% methanol. A Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) (Black) is used as isotype control. Unlabelled control : without incubation with primary antibody and secondary antibody (Blue).

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-SOX10 antibody [SP267] (AB311668)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-SOX10 antibody [SP267] (AB311668)

Immunocytochemistry/Immunofluorescence analysis of B16-F0 (mouse melanoma epithelial-like cell) labeling SOX10 with ab311668 at 1/50 (10.0 μg/mL). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% TritonX-100. ab195887 at 1/200 (2.5μg/mL) was as counterstain. Nuclear counterstain was DAPI. Confocal image showing nuclear staining in B16-F0 cells. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP267

Isotype

IgG

Conjugation

Alexa Fluor® 594

Excitation/Emission

Ex: 590nm, Em: 617nm

Carrier free

No

Reacts with

Human, Mouse

Applications

ICC/IF, Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor that plays a central role in developing and mature glia (By similarity). Specifically activates expression of myelin genes, during oligodendrocyte (OL) maturation, such as DUSP15 and MYRF, thereby playing a central role in oligodendrocyte maturation and CNS myelination (By similarity). Once induced, MYRF cooperates with SOX10 to implement the myelination program (By similarity). Transcriptional activator of MITF, acting synergistically with PAX3 (PubMed : 21965087). Transcriptional activator of MBP, via binding to the gene promoter (By similarity).
See full target information SOX10
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Version
Primary Antibodies

AB313897

Alexa Fluor® 568 Anti-SOX10 antibody [SP267]

primary-antibodies

alexa-fluor-568-sox10-antibody-sp267-ab313897

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