AB325703

Alexa Fluor® 594 Anti-TAP1 antibody [EPR26236-92]

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal TAP1 antibody - conjugated to Alexa Fluor® 594. Suitable for IHC-P and reacts with Human samples.

View Alternative Names

ABCB2, PSF1, RING4, Y3, TAP1, Antigen peptide transporter 1, APT1, ATP-binding cassette sub-family B member 2, Peptide supply factor 1, Peptide transporter PSF1, Peptide transporter TAP1, Peptide transporter involved in antigen processing 1, Really interesting new gene 4 protein, PSF-1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-TAP1 antibody [EPR26236-92] (AB325703)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-TAP1 antibody [EPR26236-92] (AB325703)

Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling TAP1 with ab325703 at 1/50 (10.0 μg/ml) dilution.

Positive staining in human colon cancer.

The primary antibody was incubated for 60 mins at room temperature (shown in magenta). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

The immunostaining was performed on a Leica Biosystems BOND RX instrument.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-TAP1 antibody [EPR26236-92] (AB325703)

Immunohistochemical analysis of paraffin-embedded (A) : HT-29 treated with IFN gamma (100 ng/mL) for 48 hours. (B) : Untreated HT-29 (human colorectal adenocarcinoma epithelial cell). tissue labeling TAP1 with ab325703 at 1/100 (5.0 μg/ml ) dilution.

Positive staining in (image A) HT-29 treated with IFN gamma (100 ng/mL) for 48 hours, nearly no staining in (image B) untreated HT-29.

The primary antibody was incubated for 60 mins at room temperature (shown in magenta). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

The immunostaining was performed on a Leica Biosystems BOND RX instrument.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-TAP1 antibody [EPR26236-92] (AB325703)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling TAP1 with ab325703 at 1/50 (10.0 μg/ml) dilution.

Positive staining in human colon.

The primary antibody was incubated for 60 mins at room temperature (shown in magenta). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

The immunostaining was performed on a Leica Biosystems BOND RX instrument.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26236-92

Isotype

IgG

Conjugation

Alexa Fluor® 594

Excitation/Emission

Ex: 590nm, Em: 617nm

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The transporter associated with antigen processing 1 (TAP1) also known as ABCB2 is an ATP-binding cassette transporter with an important role in cellular immunity. TAP1 functions mechanically by transporting peptides from the cytosol into the endoplasmic reticulum. These peptides are typically 8-16 amino acids long and are processed for MHC class I molecules presentation. TAP1 has a molecular mass of approximately 75 kDa. Expression of TAP1 occurs primarily in cells of the immune system including lymphocytes and dendritic cells.

Biological function summary

TAP1 participates in the immune system by facilitating the presentation of peptide antigens to T cells. It forms a complex with TAP2 to transport antigenic peptides necessary for the proper functioning of the MHC class I pathway. This complex is essential for the immune system's ability to detect and respond to pathogens. Proper functioning of TAP1 ensures that peptides presented on MHC class I molecules are of endogenous origin allowing T cells to monitor cellular health and detect infected or transformed cells.

Pathways

TAP1 plays a role in the antigen processing and presentation pathway which is critical for adaptive immunity. This pathway involves specific steps in peptide processing where TAP1 along with TAP2 is essential for translocating peptides for binding to MHC class I complexes. Other related proteins in this pathway include calreticulin and tapasin which assist in peptide loading onto MHC class I molecules. Additionally TAP1 is involved in regulating cytosolic proteins' turnover through its role in transporting peptides that result from degraded proteins.

TAP1 dysfunction or variants can lead to immune system-related diseases such as Bare Lymphocyte Syndrome (BLS) a condition characterized by the lack of MHC class I molecule expression. This results in compromised immune surveillance and increased susceptibility to infections. TAP1 is also linked to autoimmune diseases where abnormal peptide presentation may occur. It is associated with proteins like HLA molecules which are often implicated in autoimmune pathogenesis. Analyzing TAP1 expression and function provides insight into these disorders' underlying mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

ABC transporter associated with antigen processing. In complex with TAP2 mediates unidirectional translocation of peptide antigens from cytosol to endoplasmic reticulum (ER) for loading onto MHC class I (MHCI) molecules (PubMed : 25377891, PubMed : 25656091). Uses the chemical energy of ATP to export peptides against the concentration gradient (PubMed : 25377891). During the transport cycle alternates between 'inward-facing' state with peptide binding site facing the cytosol to 'outward-facing' state with peptide binding site facing the ER lumen. Peptide antigen binding to ATP-loaded TAP1-TAP2 induces a switch to hydrolysis-competent 'outward-facing' conformation ready for peptide loading onto nascent MHCI molecules. Subsequently ATP hydrolysis resets the transporter to the 'inward facing' state for a new cycle (PubMed : 11274390, PubMed : 25377891, PubMed : 25656091). Typically transports intracellular peptide antigens of 8 to 13 amino acids that arise from cytosolic proteolysis via IFNG-induced immunoproteasome. Binds peptides with free N- and C-termini, the first three and the C-terminal residues being critical. Preferentially selects peptides having a highly hydrophobic residue at position 3 and hydrophobic or charged residues at the C-terminal anchor. Proline at position 2 has the most destabilizing effect (PubMed : 11274390, PubMed : 7500034, PubMed : 9256420). As a component of the peptide loading complex (PLC), acts as a molecular scaffold essential for peptide-MHCI assembly and antigen presentation (PubMed : 1538751, PubMed : 25377891, PubMed : 26611325).

See full target information TAP1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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