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Rabbit Recombinant Monoclonal TPPP antibody - conjugated to Alexa Fluor® 594.

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Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

Alexa Fluor® 594

Excitation/Emission

Ex: 590nm, Em: 617nm

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Application

Target Binding Affinity

Reactivity

Expected

Dilution info

-

Notes

-

Application

Antibody Labelling

Reactivity

Expected

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Regulator of microtubule dynamics that plays a key role in myelination by promoting elongation of the myelin sheath (PubMed:31522887). Acts as a microtubule nucleation factor in oligodendrocytes: specifically localizes to the postsynaptic Golgi apparatus region, also named Golgi outpost, and promotes microtubule nucleation, an important step for elongation of the myelin sheath (PubMed:31522887, PubMed:33831707). Required for both uniform polarized growth of distal microtubules as well as directing the branching of proximal processes (PubMed:31522887). Shows magnesium-dependent GTPase activity; the role of the GTPase activity is unclear (PubMed:21316364, PubMed:21995432). In addition to microtubule nucleation activity, also involved in microtubule bundling and stabilization of existing microtubules, thereby maintaining the integrity of the microtubule network (PubMed:17105200, PubMed:17693641, PubMed:18028908, PubMed:26289831). Regulates microtubule dynamics by promoting tubulin acetylation: acts by inhibiting the tubulin deacetylase activity of HDAC6 (PubMed:20308065, PubMed:23093407). Also regulates cell migration: phosphorylation by ROCK1 inhibits interaction with HDAC6, resulting in decreased acetylation of tubulin and increased cell motility (PubMed:23093407). Plays a role in cell proliferation by regulating the G1/S-phase transition (PubMed:23355470). Involved in astral microtubule organization and mitotic spindle orientation during early stage of mitosis; this process is regulated by phosphorylation by LIMK2 (PubMed:22328514).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal TPPP antibody - conjugated to Alexa Fluor® 594.

Key facts

Isotype

IgG

Conjugation

Alexa Fluor® 594

Excitation/Emission

Ex: 590nm, Em: 617nm

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR3316

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle, Store in the dark

Notes

This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone. This conjugated antibody is eligible for the Abcam trial program.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

TPPP also known as Tubulin Polymerization Promoting Protein or p25 is a small protein with an approximate mass of 25 kDa. TPPP is expressed mainly in the brain particularly abundant in oligodendrocytes. This protein plays an important role in regulating the polymerization of tubulin into microtubules. Through its binding to tubulin TPPP stabilizes microtubule structures facilitating proper microtubule assembly and maintenance. The modulation of these structures is essential for cellular operations like intracellular transport and cell division.

Biological function summary

The protein participates in the dynamics of cytoskeletal elements critical for maintaining cell shape and function. TPPP is part of cytoskeletal networks where it contributes to cellular integrity in neurons. TPPP's activity influences the formation and maintenance of myelin the insulating layer enveloping nerve fibers which is important for fast and efficient nerve impulse conduction. In its role TPPP interacts cooperatively with other cytoskeletal proteins enhancing microtubule resilience and cell structural stability.

Pathways

TPPP interacts in processes involving microtubule organization and stabilization. It is integral in the MAPK/ERK signaling pathway which is prominent in regulating cellular processes like proliferation and differentiation. Additionally TPPP may interplay with proteins such as MAP2 and Tau both involved in the MAP-kinase pathways regulating microtubule dynamics indicating a collaborative network in cellular signaling and structural reinforcement in neural cells.

Associated diseases and disorders

TPPP has correlations with neurodegenerative diseases such as Parkinson's and Alzheimer's diseases. Abnormal regulation or aggregation of TPPP is linked to the formation of pathological protein inclusions found in these conditions. The interaction between TPPP and Alpha-synuclein is particularly notable as TPPP may regulate or enhance the aggregation of Alpha-synuclein contributing to the development of Lewy bodies in Parkinson's. Understanding these interactions and pathways is essential for developing therapeutic strategies targeting these neurodegenerative disorders.

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