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AB323464

Alexa Fluor® 594 Anti-Transaldolase 1 antibody [EPR28140-51]

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Rabbit Recombinant Monoclonal Transaldolase 1 antibody - conjugated to Alexa Fluor® 594. Suitable for IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

View Alternative Names

TAL, TALDO, TALDOR, TALDO1, Transaldolase

8 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-Transaldolase 1 antibody [EPR28140-51] (AB323464)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-Transaldolase 1 antibody [EPR28140-51] (AB323464)

Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue labeling Transaldolase 1 with ab323464 at 1/100 (5.0 ug/ml) dilution.

Positive staining on human liver cancer.
The section was incubated with ab323464 for 60 mins at room temperature (shown in magenta). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-Transaldolase 1 antibody [EPR28140-51] (AB323464)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-Transaldolase 1 antibody [EPR28140-51] (AB323464)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Transaldolase 1 with ab323464 at 1/100 (5.0 ug/ml) dilution.

Positive staining on human liver.
The section was incubated with ab323464 for 60 mins at room temperature (shown in magenta). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

Flow Cytometry (Intracellular) - Alexa Fluor® 594 Anti-Transaldolase 1 antibody [EPR28140-51] (AB323464)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 594 Anti-Transaldolase 1 antibody [EPR28140-51] (AB323464)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized A431 (human epidermoid carcinoma epithelial cell) cells labelling Transaldolase 1 with ab323464 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Transaldolase 1 antibody [EPR28140-51] (AB323464)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Transaldolase 1 antibody [EPR28140-51] (AB323464)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A431 (human epidermoid carcinoma epithelial cell) cells labelling Transaldolase 1 with ab323464 at 1/50 (10.0 ug/ml) dilution (Green).

Confocal image showing nuclear staining in A431 cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-Transaldolase 1 antibody [EPR28140-51] (AB323464)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-Transaldolase 1 antibody [EPR28140-51] (AB323464)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Transaldolase 1 with ab323464 at 1/100 (5.0 ug/ml) dilution.

Positive staining on mouse liver.
The section was incubated with ab323464 for 60 mins at room temperature (shown in magenta). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Transaldolase 1 antibody [EPR28140-51] (AB323464)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Transaldolase 1 antibody [EPR28140-51] (AB323464)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling Transaldolase 1 with ab323464 at 1/50 (10.0 ug/ml) dilution (Green).

Confocal image showing nuclear staining in RAW 264.7 cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Flow Cytometry (Intracellular) - Alexa Fluor® 594 Anti-Transaldolase 1 antibody [EPR28140-51] (AB323464)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 594 Anti-Transaldolase 1 antibody [EPR28140-51] (AB323464)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling Transaldolase 1 with ab323464 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 594) (ab208568) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-Transaldolase 1 antibody [EPR28140-51] (AB323464)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-Transaldolase 1 antibody [EPR28140-51] (AB323464)

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Transaldolase 1 with ab323464 at 1/100 (5.0 ug/ml) dilution.

Positive staining on rat liver.
The section was incubated with ab323464 for 60 mins at room temperature (shown in magenta). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28140-51

Isotype

IgG

Conjugation

Alexa Fluor® 594

Excitation/Emission

Ex: 590nm, Em: 617nm

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

ICC/IF, IHC-P, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Transaldolase 1 also known as TALDO1 or TAL-H is an enzyme that plays an important role in the pentose phosphate pathway by catalyzing the transfer of a three-carbon dihydroxyacetone unit from sedoheptulose 7-phosphate to glyceraldehyde 3-phosphate producing erythrose 4-phosphate and fructose 6-phosphate. The molecular mass of Transaldolase 1 is approximately 37 kDa. It is expressed in various tissues within the body with higher expression levels observed in the liver and adipose tissues.
Biological function summary

Transaldolase 1 regulates the balance between the oxidative and non-oxidative branches of the pentose phosphate pathway essential for nucleotide biosynthesis and maintaining cellular redox status. It functions independently not as part of a larger enzyme complex. The activity of this enzyme affects cellular metabolic functions impacting the synthesis of nucleotides and pentose sugars which are necessary for cell growth and division.

Pathways

Transaldolase 1 is integral to the pentose phosphate pathway also known as the hexose monophosphate shunt. This pathway is important for providing ribose-5-phosphate for nucleotide synthesis and NADPH for reductive biosynthesis and cellular antioxidant systems. It links with glycolysis through shared intermediates such as fructose 6-phosphate connecting with enzymes like glucose-6-phosphate dehydrogenase. This relationship allows cells to adjust their metabolic flux according to physiological needs.

Transaldolase 1 mutations are associated with transaldolase deficiency a rare metabolic disorder that can lead to liver dysfunction and immunodeficiency. This deficiency affects the cellular ability to balance nucleotide and lipid biosynthesis contributing to the disease phenotype. There is also a connection with oxidative stress-related disorders as Transaldolase 1 indirectly influences reactive oxygen species via its role in NADPH production which ties it to proteins involved in oxidative stress responses like NADPH oxidase.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the rate-limiting step of the non-oxidative phase in the pentose phosphate pathway. Catalyzes the reversible conversion of sedheptulose-7-phosphate and D-glyceraldehyde 3-phosphate into erythrose-4-phosphate and beta-D-fructose 6-phosphate (PubMed : 18687684, PubMed : 8955144). Not only acts as a pentose phosphate pathway enzyme, but also affects other metabolite pathways by altering its subcellular localization between the nucleus and the cytoplasm (By similarity).
See full target information TALDO1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com