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AB318745

Alexa Fluor® 594 Anti-TrkB antibody [EPR1294]

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Rabbit Recombinant Monoclonal TrkB antibody - conjugated to Alexa Fluor® 594. Suitable for Target Binding Affinity, Antibody Labelling, IHC-P and reacts with Human samples.

View Alternative Names

TRKB, NTRK2, BDNF/NT-3 growth factors receptor, GP145-TrkB, Neurotrophic tyrosine kinase receptor type 2, TrkB tyrosine kinase, Tropomyosin-related kinase B, Trk-B

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1294

Isotype

IgG

Conjugation

Alexa Fluor® 594

Excitation/Emission

Ex: 590nm, Em: 617nm

Carrier free

No

Reacts with

Human

Applications

IHC-P, Antibody Labelling, Target Binding Affinity

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Assay Kits

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ELISA - Human TrkB ELISA Kit (AB264625)

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Primary Antibodies

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Secondary Antibodies

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Primary Antibodies

AB125011

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "TargetBindingAffinity" : {"fullname" : "Target Binding Affinity", "shortname":"Target Binding Affinity"}, "AntibodyLabelling" : {"fullname" : "Antibody Labelling", "shortname":"Antibody Labelling"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "TargetBindingAffinity-species-checked": "guaranteed", "TargetBindingAffinity-species-dilution-info": "", "TargetBindingAffinity-species-notes": "", "AntibodyLabelling-species-checked": "guaranteed", "AntibodyLabelling-species-dilution-info": "", "AntibodyLabelling-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TrkB also known as Neurotrophic Tyrosine Kinase Receptor Type 2 (NTRK2) is a cell surface receptor with a mass of approximately 145 kDa. It binds brain-derived neurotrophic factor (BDNF) and is widely expressed in the central nervous system particularly in areas like the hippocampus cortex and cerebellum. TrkB belongs to the family of receptor tyrosine kinases functioning by catalyzing the transfer of a phosphate group from ATP to specific tyrosine residues on substrate proteins in the receptor triggering downstream signaling cascades.
Biological function summary

TrkB plays essential roles in neuronal survival differentiation and synaptic plasticity. It acts as an important mediator for the effects of neurotrophins primarily BDNF on neurons. While TrkB itself is not part of a multi-protein complex it interacts closely with several adaptor proteins post-activation to propagate survival and proliferation signals within the neuron. These biochemical interactions greatly influence neurodevelopment and cognitive functions.

Pathways

TrkB is a pivotal player in both the MAPK/ERK and PI3K/AKT pathways. Upon BDNF binding TrkB undergoes autophosphorylation triggering these pathways which are critically involved in cell survival and growth. Other proteins such as SHC1 and PLCγ interact with TrkB in these pathways extending the range of cellular responses to neurotrophin signaling facilitating processes like neuronal plasticity and memory formation.

TrkB has significant implications in neurological conditions like Alzheimer's disease and depression. In Alzheimer's disease alterations in TrkB signaling can result in impaired cognitive functions and neuronal loss where it interacts with proteins like amyloid-beta contributing to disease progression. In depression dysfunctional BDNF-TrkB signaling impacts mood regulation and resilience linking TrkB with alterations in serotonin receptor signaling emphasizing its role in mood disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity (By similarity). Receptor for BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin-4. Alternatively can also bind NTF3/neurotrophin-3 which is less efficient in activating the receptor but regulates neuron survival through NTRK2 (PubMed : 15494731, PubMed : 7574684). Upon ligand-binding, undergoes homodimerization, autophosphorylation and activation (PubMed : 15494731). Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades. Through SHC1, FRS2, SH2B1, SH2B2 activates the GRB2-Ras-MAPK cascade that regulates for instance neuronal differentiation including neurite outgrowth. Through the same effectors controls the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. Through PLCG1 and the downstream protein kinase C-regulated pathways controls synaptic plasticity. Thereby, plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. Hence, it is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. May also play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia.
See full target information NTRK2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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