AB154207

Alexa Fluor® 594 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

RabMAb
Recombinant
KO Validated
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(20 Publications)

Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker - Alexa Fluor® 594 conjugated (ab154207) is a rabbit recombinant monoclonal antibody detecting Vimentin in Flow Cytometry (Intra), ICC/IF. Suitable for Human, Mouse.

- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications

View Alternative Names

Vimentin, VIM

3 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB154207)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB154207)

ab154207 staining Vimentin in wild-type HAP1 cells (top panel) and Vimentin knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab154207 at 1/1000 dilution (shown in red) and ab195887 at 1/250 dilution (shown in green) overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Flow Cytometry (Intracellular) - Alexa Fluor® 594 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB154207)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Alexa Fluor® 594 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB154207)

Overlay histogram showing HAP1 wildtype (green line) and HAP1-VIM knockout cells (red line) stained with ab154207. The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab154207, 0.1μg/ml dilution) for 30 min at 22°C.

A rabbit monoclonal IgG isotype control antibody (ab208568) was used at the same concentration and conditions as the primary antibody (HAP1 wildtype - black line, HAP1-VIM knockout - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (488nm) and 630/30 bandpass filter.

This antibody can also be used in HAP1 cells fixed with 80% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 15 min under the same conditions.

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB154207)

ab154207 staining Vimentin in HeLa cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab154207 at a working dilution of 1 in 100 (shown in pseudo color red) and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin) at 1μg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an AlexaFluor^® 488 Goat anti-Mouse secondary (ab150117) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

HRP

HRP Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker
421 Alexa Fluor® 405

Alexa Fluor® 405 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker
578 PE

PE Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker
660 APC

APC Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker
Unconjugated

Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker
Carrier free

Anti-Vimentin antibody [EPR3776] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3776

Isotype

IgG

Conjugation

Alexa Fluor® 594

Excitation/Emission

Ex: 590nm, Em: 617nm

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, Flow Cyt (Intra), IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/0.1", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

What is this antibody validated in?
Alexa Fluor® 594 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab154207) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse samples.

Trusted by the scientific community
Anti-Vimentin [EPR3776] - Cytoskeleton Marker Alexa Fluor® 594 (ab154207) was first used in a scientific publication in 2012 and has been cited over 10 times in peer-reviewed journals.

Related products
Antibody clone EPR3776 is also available pre-conjugated to a variety of labels for your convenience – Anti-Vimentin Alexa Fluor® 594 [EPR3776] - Cytoskeleton Marker (ab154207).

Specificity confirmed
The specificity of Alexa Fluor® 594 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab154207) has been confirmed by Immunocytochemistry testing in VIM Knockout HAP1 cells.

Other related products
We have a range of other formats of antibody clone [EPR3776] also available for your convenience: ab92547, Alexa Fluor® 594 - ab154207, Alexa Fluor® 488 - ab185030, Carrier free - ab193555, HRP - ab194718, Alexa Fluor® 647 - ab194719, Alexa Fluor® 568 - ab202504, Alexa Fluor® 555 - ab203428, PE - ab209446, Alexa Fluor® 405 - ab210152, Alexa Fluor® 568 - ab236866, Carrier free - ab307988, APC - ab314250, ab316214, Carrier free - ab316215, Alexa Fluor® 750 - ab321763, ab323238, Carrier free - ab323247

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front (By similarity). Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner (By similarity). May promote axon outgrowth and motor fiber repair via DSP-mediated recruitment to outgrowth tips (By similarity).. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

See full target information VIM

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Publications (20)

Recent publications for all applications. Explore the full list and refine your search

CytoJournal 22:46 PubMed40469704

2025

Hydrogen gas therapy: A promising approach for sepsis management post-burn injury by modulating inflammation, oxidative stress, and wound healing.

Applications

Unspecified application

Species

Unspecified reactive species

Pan Yu,Nan Hong,Genwang Wang,Shuqiang Chen,Zhipeng Zhao

Immunotherapy advances 3:ltad015 PubMed37636242

2023

Lytic efficiency of immunosuppressive drug-resistant armoured T cells against circulating HBV-related HCC in whole blood.

Applications

Unspecified application

Species

Unspecified reactive species

Meiyin Lin,Sebastian Chakrit Bhakdi,Damien Tan,Joycelyn Jie Xin Lee,David Wai Meng Tai,Andrea Pavesi,Lu-En Wai,Tina Wang,Antonio Bertoletti,Anthony Tanoto Tan

Med (New York, N.Y.) 4:554-579.e9 PubMed37572651

2023

Organoid co-culture model of the human endometrium in a fully synthetic extracellular matrix enables the study of epithelial-stromal crosstalk.

Applications

Unspecified application

Species

Unspecified reactive species

Juan S Gnecco,Alexander Brown,Kira Buttrey,Clara Ives,Brittany A Goods,Lauren Baugh,Victor Hernandez-Gordillo,Megan Loring,Keith B Isaacson,Linda G Griffith

Cancer research communications 3:640-658 PubMed37082579

2023

Identification of Membrane-expressed CAPRIN-1 as a Novel and Universal Cancer Target, and Generation of a Therapeutic Anti-CAPRIN-1 Antibody TRK-950.

Applications

Unspecified application

Species

Unspecified reactive species

Fumiyoshi Okano,Takanori Saito,Yoshitaka Minamida,Shinichi Kobayashi,Takayoshi Ido,Yasushi Miyauchi,Ukei Wasai,Daisuke Akazawa,Masahiko Kume,Masaki Ishibashi,Ke Jiang,Alexandra Aicher,Christopher Heeschen,Tetsu Yonehara

Arthritis & rheumatology (Hoboken, N.J.) 74:860-870 PubMed34882985

2022

Therapeutic Effect of Cyclin-Dependent Kinase 4/6 Inhibitor on Dermal Fibrosis in Murine Models of Systemic Sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Akio Yamamoto,Tetsuya Saito,Tadashi Hosoya,Kimito Kawahata,Yoshihide Asano,Shinichi Sato,Fumitaka Mizoguchi,Shinsuke Yasuda,Hitoshi Kohsaka

Cellular and molecular neurobiology 43:251-264 PubMed34853925

2021

FAM3A Ameliorates Brain Impairment Induced by Hypoxia-Ischemia in Neonatal Rat.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Song,Qingying Gao,Taotao Chen,Ting Wen,Peng Wu,Xiao Luo,Qiao Yi Chen

Life sciences 259:118390 PubMed32896556

2020

ABCA1 plays an anti-inflammatory role by affecting TLR4 at the feto-maternal interface.

Applications

Unspecified application

Species

Unspecified reactive species

Ning Ding,Na Liu,Lei Yang,Xiaoyan Han,Li Lin,Yan Long

Journal of tissue engineering 11:2041731420920597 PubMed32489578

2020

The anti-angiogenic tyrosine kinase inhibitor Pazopanib kills cancer cells and disrupts endothelial networks in biomimetic three-dimensional renal tumouroids.

Applications

Unspecified application

Species

Unspecified reactive species

Katerina Stamati,Patricia A Redondo,Agata Nyga,Joana B Neves,Maxine Gb Tran,Mark Emberton,Umber Cheema,Marilena Loizidou

Laboratory investigation; a journal of technical m 100:1003-1013 PubMed32005909

2020

Generation and characterization of a cell line from an intraductal tubulopapillary neoplasm of the pancreas.

Applications

Unspecified application

Species

Unspecified reactive species

Matthäus Felsenstein,Maria A Trujillo,Bo Huang,Neha Nanda,Zhengdong Jiang,Yea Ji Jeong,Michael Pflüger,Michael G Goggins,Ralph H Hruban,Elizabeth D Thompson,Christopher M Heaphy,Nicholas J Roberts,Laura D Wood

Nature nanotechnology 15:154-161 PubMed31988506

2020

Pro-efferocytic nanoparticles are specifically taken up by lesional macrophages and prevent atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Alyssa M Flores,Niloufar Hosseini-Nassab,Kai-Uwe Jarr,Jianqin Ye,Xingjun Zhu,Robert Wirka,Ai Leen Koh,Pavlos Tsantilas,Ying Wang,Vivek Nanda,Yoko Kojima,Yitian Zeng,Mozhgan Lotfi,Robert Sinclair,Irving L Weissman,Erik Ingelsson,Bryan Ronain Smith,Nicholas J Leeper

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 594 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB154207)

Flow Cytometry (Intracellular) - Alexa Fluor® 594 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB154207)

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB154207)

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Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (20)

Product promise