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AB319685

Alexa Fluor® 647 Anti-0N Tau antibody [EPR21726]

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Rabbit Recombinant Monoclonal TAU antibody - conjugated to Alexa Fluor® 647.

View Alternative Names

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

  • Unconjugated

    Anti-0N Tau antibody [EPR21726]

  • Carrier free

    Anti-0N Tau antibody [EPR21726] - BSA and Azide free

  • 578 PE

    PE Anti-0N Tau antibody [EPR21726]

  • 660 APC

    APC Anti-0N Tau antibody [EPR21726]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-0N Tau antibody [EPR21726]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-0N Tau antibody [EPR21726]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-0N Tau antibody [EPR21726]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-0N Tau antibody [EPR21726]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21726

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Applications

Target Binding Affinity, Antibody Labelling

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The specificity of this antibody refers to P10636-2.

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 0N Tau protein also known by its alternate names MAPT (microtubule-associated protein tau) and non-N-terminal tau serves important mechanical functions in the stabilization of microtubules within neurons. It does so by binding to and promoting microtubule assembly and maintenance. The mass of the 0N Tau isoform varies because of alternative splicing but it is a critical component of neurofibrillary structures. This protein expression occurs primarily in neurons but can also be found in glial cells of the central nervous system.
Biological function summary

The stabilization function of the 0N Tau isoform influences the cellular cytoskeleton's structural integrity which is necessary for accurate neuronal transport and function. It participates in the microtubule complex which forms the network necessary for intracellular transport and structural support. Stability and dynamics of this network allow efficient transport of nutrients organelles and signaling molecules within the cells playing an important role in maintaining neuronal health and function.

Pathways

The 0N Tau protein is involved in critical pathways like the MAPK and PI3K-Akt signaling pathways. These pathways influence cell survival proliferation and apoptotic processes. Proteins like glycogen synthase kinase-3 beta (GSK-3β) regulate phosphorylation of tau affecting its binding to microtubules and subsequent cellular dynamics. Additionally hyperphosphorylation of tau by kinases such as CDK5 modifies its function and alters cellular pathways.

The role of 0N Tau becomes significant in neurodegenerative conditions like Alzheimer's disease and frontotemporal dementia. These disorders are associated with aberrant phosphorylation and aggregation of tau resulting in pathological tau tangles. The relationship of 0N Tau with amyloid-beta peptides also links it to Alzheimer's disease further highlighting its involvement in neurodegeneration. Proteins like amyloid precursor protein (APP) connect with tau pathology within these diseases contributing to neuronal dysfunction and degeneration.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The protein expressed by the MAPT gene promotes microtubule assembly and stability and might be involved in establishing and maintaining neuronal polarity. Its C-terminus binds axonal microtubules, and the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between the two. Axonal polarity is predetermined by MAPT localization within the neuronal cell's domain defined by the centrosome. The short isoforms allow cytoskeleton plasticity, whereas the longer isoforms may preferentially play a role in its stabilization. This supplementary information is collated from multiple sources and compiled automatically.
See full target information MAPT

Additional targets

MAPT
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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