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Knockout Tested Rabbit Recombinant Monoclonal NFM antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF, IHC-Fr and reacts with Human, Mouse samples.

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Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-160 kD Neurofilament Medium antibody [EPR23510-76] (AB317599), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-160 kD Neurofilament Medium antibody [EPR23510-76] (AB317599), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-160 kD Neurofilament Medium antibody [EPR23510-76] (AB317599), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIHC-Fr
Human
Tested
Expected
Mouse
Expected
Tested

Tested
Tested

Species

Human

Dilution info

1 µg/mL

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/100

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Target data

Function

Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).

Alternative names

Knockout Tested Rabbit Recombinant Monoclonal NFM antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF, IHC-Fr and reacts with Human, Mouse samples.

Key facts

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR23510-76

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The 160 kD Neurofilament Medium also known as NEFM NF-M or NEF-M belongs to the family of neurofilament proteins. This protein is an important structural component of neurons particularly in the axonal cytoskeleton. Neurofilament Medium has a molecular mass of around 160 kilodaltons (kD). It shows high levels of expression in neuronal tissues contributing to the maintenance of axonal caliber. Commonly research recognizes it alongside other neurofilament proteins emphasizing its role in providing structural integrity to neurons.

Biological function summary

Neurofilament Medium participates in the formation of a stable network of neurofilaments within neurons. Acting as a part of the intermediate filament protein family it forms a complex with other neurofilaments such as NF-L and NF-H. This complex supports neuron structure and plays an important role in axonal transport. The heteropolymeric nature of neurofilaments contributes to their mechanical stability facilitating essential neuronal functions by aligning neurofilaments along the axon.

Pathways

Neurofilament Medium integrates into the cytoskeletal arrangement pathways that govern axonal transport and stability. It plays a significant role in the neuronal transport pathway associating with other proteins such as dynein and kinesin responsible for the motor functions along axons. Also it relates to the MAP kinase pathway where phosphorylation events modulate its assembly and disassembly dynamics in response to cellular signals.

Associated diseases and disorders

Neurofilament Medium closely relates to neurodegenerative conditions such as amyotrophic lateral sclerosis (ALS) and Alzheimer's disease. Aberrant phosphorylation or aggregation of NEFM can disrupt neuronal function leading to pathology. In ALS the abnormal accumulation of neurofilaments correlates with motor neuron degeneration. In Alzheimer's its interaction with tau protein poses significant interest as altered states of either could exacerbate neurofibrillary tangles worsening cognitive decline.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-160 kD Neurofilament Medium antibody [EPR23510-76] (ab317599), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-160 kD Neurofilament Medium antibody [EPR23510-76] (ab317599)

    ab317599 staining Anti-160 kD Neurofilament Medium in Hek-293T cells, with negative expression in NEFM knockout Hek-293T cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised with 0.1% Triton x-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab317599 at 1 μg/ml (shown in green). Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488) (shown in magenta) was used at 2 μg/ml for structural counterstaining.

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

    This product also works with 100% methanol (5 min) fixation under the same testing conditions.

  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-160 kD Neurofilament Medium antibody [EPR23510-76] (ab317599), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-160 kD Neurofilament Medium antibody [EPR23510-76] (ab317599)

    Negative immunofluorescence staining of 160 kD Neurofilament Medium in a section of frozen mouse normal liver.

    The section was fixed using 10% formaldehyde in 1X PBS for 10 minutes. No antigen retrieval step was performed prior to staining. Performed on a Leica BOND™. The section was incubated at room temperature for 1 hour with ab317599 at 1/100 dilution. Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-160 kD Neurofilament Medium antibody [EPR23510-76] (ab317599), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-160 kD Neurofilament Medium antibody [EPR23510-76] (ab317599)

    Immunofluorescence staining of 160 kD Neurofilament Medium in a section of frozen mouse normal cerebellum.

    The section was fixed using 10% formaldehyde in 1X PBS for 10 minutes. No antigen retrieval step was performed prior to staining. Performed on a Leica BOND™. The section was incubated at room temperature for 1 hour with ab317599 at 1/100 dilution (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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