AB237337

Alexa Fluor® 647 Anti-6X His tag® antibody [EPR20547]

Name: Alexa Fluor® 647 conjugated Anti-6X His tag® antibody [EPR20547] (ab237337) | Abcam
Brand: Abcam Limited
SKU: AB237337
Price: 575 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(8 Publications)

Anti-6X His tag® antibody [EPR20547] - Alexa Fluor® 647 conjugated (ab237337) is a rabbit recombinant monoclonal antibody detecting 6X His tag® in ICC/IF.

- Biophysical QC for unrivalled batch-batch consistency

1 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-6X His tag® antibody [EPR20547] (AB237337)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-6X His tag® antibody [EPR20547] (AB237337)

ab237337 staining 6X His tag^® in 293T cells transfected with 6X His tag^® with GFP tag. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab237337 at 1/100 dilution (shown in red). Nuclear DNA was labeled with DAPI (shown in blue) and GFP is shown in green.

578 PE

PE Anti-6X His tag® antibody [EPR20547]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-6X His tag® antibody [EPR20547]
HRP

HRP Anti-6X His tag® antibody [EPR20547]
Biotin

Biotin Anti-6X His tag® antibody [EPR20547] - ChIP Grade
660 APC

APC Anti-6X His tag® antibody [EPR20547] - ChIP Grade
519 FITC

FITC Anti-6X His tag® [EPR20547]
Unconjugated

Anti-6X His tag® antibody [EPR20547] - ChIP Grade
Carrier free

Anti-6X His tag® antibody [EPR20547] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20547

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in 6x His tag with GFP tag transfected 293T fixed with 4% formaldehyde (10 min).</p>" } } }

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Product details

What is this antibody validated in?
Alexa Fluor® 647 Anti-6X His tag® antibody [EPR20547] (ab237337) is a rabbit recombinant monoclonal antibody and is validated for use in Immunocytochemistry/immunofluorescence (ICC/IF). Tagged protein detection
6X His tag® antibodies are used to visualize proteins labelled with this tag in a variety of applications.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Related products
Antibody clone EPR20547 is also available pre-conjugated to a variety of labels for your convenience – Anti-6X His tag® Alexa Fluor® 647 [EPR20547] (ab237337).

Other related products
We have a range of other formats of antibody clone [EPR20547] also available for your convenience: ab213204, Carrier free - ab232492, Alexa Fluor® 488 - ab237336, Alexa Fluor® 647 - ab237337, PE - ab237338, HRP - ab237339, Biotin - ab316173, APC - ab317659, FITC - ab318311

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 6X His tag is a sequence commonly used for protein purification detection and immobilization. It consists of six histidine residues that bind to metal ions like nickel or cobalt. This makes it a highly efficient tag for affinity chromatography. His tag antibodies such as anti-His HRP facilitate the detection of His-tagged proteins in various assays. Being a small tag with a typical molecular weight of about 0.84 kDa it minimally interferes with protein function. Many proteins expressed in E. coli and other expression systems carry the 6X His tag for ease of purification and analysis.

Biological function summary

Proteins tagged with the 6X His tag often play roles in studies involving recombinant proteins. The tag itself does not alter the biological function of the target protein. It is not part of any natural protein complex but aids in the study of such complexes. Researchers use His tag antibodies like anti-His to specifically target and study these tagged proteins without cross-reactivity to other proteins.

Pathways

The incorporation of the 6X His tag into proteins allows them to be studied more efficiently in various biological pathways. It can be part of pathways like signal transduction or metabolic pathways. When tagged proteins interact with other molecules such as kinases or receptors researchers can track these interactions using the tag and anti-Histidine antibodies. This technique informs on proteins that are hard to purify due to low abundance or instability.

The 6X His tag itself does not have direct implications. However proteins of interest in disease models often carry the tag which allows researchers to purify and study them in detail. For example cancer-related proteins may be expressed with a His tag to understand their role in tumorigenesis. Similarly proteins involved in metabolic disorders could be tagged which helps in analyzing specific interactions or functions disrupted in diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information 6X His tag®

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine (Cambridge, Mass.) 31:170 PubMed40325370

2025

Bacteriophage-derived endolysins restore antibiotic susceptibility in β-lactam- and macrolide-resistant Streptococcus pneumoniae infections.

Applications

Unspecified application

Species

Unspecified reactive species

Niels Vander Elst,Kristine Farmen,Lisa Knörr,Lotte Merlijn,Federico Iovino

PLoS pathogens 20:e1012318 PubMed38865374

2024

Arboviruses antagonize insect Toll antiviral immune signaling to facilitate the coexistence of viruses with their vectors.

Applications

Unspecified application

Species

Unspecified reactive species

Dongsheng Jia,Guozhong Luo,Heran Guan,Tingting Yu,Xinyan Sun,Yu Du,Yiheng Wang,Hongyan Chen,Taiyun Wei

PLoS genetics 20:e1011127 PubMed38829907

2024

The conserved σD envelope stress response monitors multiple aspects of envelope integrity in corynebacteria.

Applications

Unspecified application

Species

Unspecified reactive species

Elizabeth M Hart,Evan Lyerly,Thomas G Bernhardt

The Journal of neuroscience : the official journal of the Society for Neuroscience 44: PubMed38383499

2024

HERV-K (HML-2) Envelope Protein Induces Mitochondrial Depolarization and Neurotoxicity via Endolysosome Iron Dyshomeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Peter W Halcrow,Darius N K Quansah,Nirmal Kumar,Joseph P Steiner,Avindra Nath,Jonathan D Geiger

Nature communications 14:1606 PubMed36959204

2023

Modular-designed engineered bacteria for precision tumor immunotherapy via spatiotemporal manipulation by magnetic field.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaotu Ma,Xiaolong Liang,Yao Li,Qingqing Feng,Keman Cheng,Nana Ma,Fei Zhu,Xinjing Guo,Yale Yue,Guangna Liu,Tianjiao Zhang,Jie Liang,Lei Ren,Xiao Zhao,Guangjun Nie

International journal of molecular sciences 23: PubMed35054983

2022

Syndecan-4 Is a Key Facilitator of the SARS-CoV-2 Delta Variant's Superior Transmission.

Applications

Unspecified application

Species

Unspecified reactive species

Anett Hudák,Gábor Veres,Annamária Letoha,László Szilák,Tamás Letoha

Nature communications 12:6161 PubMed34697321

2021

Functionality of the putative surface glycoproteins of the Wuhan spiny eel influenza virus.

Applications

Unspecified application

Species

Unspecified reactive species

Guha Asthagiri Arunkumar,Disha Bhavsar,Tiehai Li,Shirin Strohmeier,Veronika Chromikova,Fatima Amanat,Mehman Bunyatov,Patrick C Wilson,Ali H Ellebedy,Geert-Jan Boons,Viviana Simon,Robert P de Vries,Florian Krammer

International journal of molecular sciences 22: PubMed34069441

2021

Contribution of Syndecans to the Cellular Entry of SARS-CoV-2.

Applications

Unspecified application

Species

Unspecified reactive species

Anett Hudák,Annamária Letoha,László Szilák,Tamás Letoha

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 647 Anti-6X His tag® antibody [EPR20547]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-6X His tag® antibody [EPR20547] (AB237337)

Related conjugates and formulations (8)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise