Rabbit Recombinant Monoclonal 6X His tag® antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Tag - Human samples. Cited in 3 publications.
Images
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-6X His tag® antibody [EPR20547] (AB237337), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-647-6x-his-tag-antibody-epr20547-ab237337--immunocytochemistry-immunofluorescence-img109103.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-6X His tag® antibody [EPR20547] (AB237337)")
Publications
- Nature communications 14:16062023Modular-designed engineered bacteria for precision tumor immunotherapy via spatiotemporal manipulation by magnetic field.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaotu Ma,Xiaolong Liang,Yao Li,Qingqing Feng,Keman Cheng,Nana Ma,Fei Zhu,Xinjing Guo,Yale Yue,Guangna Liu,Tianjiao Zhang,Jie Liang,Lei Ren,Xiao Zhao,Guangjun NiePubMed 36959204
- International journal of molecular sciences 23:2022Syndecan-4 Is a Key Facilitator of the SARS-CoV-2 Delta Variant's Superior Transmission.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnett Hudák,Gábor Veres,Annamária Letoha,László Szilák,Tamás LetohaPubMed 35054983
Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
Alexa Fluor® 647
- Excitation/Emission
Ex: 650nm, Em: 665nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | |
|---|---|
| Tag - Human | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag - Human | Dilution info 1/100 | Notes This product gave a positive signal in 6x His tag with GFP tag transfected 293T fixed with 4% formaldehyde (10 min). |
Associated Products
Select an associated product type
8 products for Alternative Product
7 products for Alternative Version
Target data
Alternative names
6 His epitope tag, HHHHHH epitope tag, HHHHHH tag, Hexa His tag, Polyhistidine Tag
Recommended products
Rabbit Recombinant Monoclonal 6X His tag® antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Tag - Human samples. Cited in 3 publications.
Alternative names
6 His epitope tag, HHHHHH epitope tag, HHHHHH tag, Hexa His tag, Polyhistidine Tag
Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
Alexa Fluor® 647
- Excitation/Emission
Ex: 650nm, Em: 665nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR20547
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Supplementary info
- Activity summary
The 6X His tag is a sequence commonly used for protein purification detection and immobilization. It consists of six histidine residues that bind to metal ions like nickel or cobalt. This makes it a highly efficient tag for affinity chromatography. His tag antibodies such as anti-His HRP facilitate the detection of His-tagged proteins in various assays. Being a small tag with a typical molecular weight of about 0.84 kDa it minimally interferes with protein function. Many proteins expressed in E. coli and other expression systems carry the 6X His tag for ease of purification and analysis.
- Biological function summary
Proteins tagged with the 6X His tag often play roles in studies involving recombinant proteins. The tag itself does not alter the biological function of the target protein. It is not part of any natural protein complex but aids in the study of such complexes. Researchers use His tag antibodies like anti-His to specifically target and study these tagged proteins without cross-reactivity to other proteins.
- Pathways
The incorporation of the 6X His tag into proteins allows them to be studied more efficiently in various biological pathways. It can be part of pathways like signal transduction or metabolic pathways. When tagged proteins interact with other molecules such as kinases or receptors researchers can track these interactions using the tag and anti-Histidine antibodies. This technique informs on proteins that are hard to purify due to low abundance or instability.
- Associated diseases and disorders
The 6X His tag itself does not have direct implications. However proteins of interest in disease models often carry the tag which allows researchers to purify and study them in detail. For example cancer-related proteins may be expressed with a His tag to understand their role in tumorigenesis. Similarly proteins involved in metabolic disorders could be tagged which helps in analyzing specific interactions or functions disrupted in diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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Terms & Conditions.
1 product image
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-6X His tag® antibody [EPR20547] (ab237337), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-647-6x-his-tag-antibody-epr20547-ab237337--immunocytochemistry-immunofluorescence-img109103.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-6X His tag® antibody [EPR20547] (ab237337)")
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-6X His tag® antibody [EPR20547] (ab237337)
ab237337 staining 6X His tag® in 293T cells transfected with 6X His tag® with GFP tag. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab237337 at 1/100 dilution (shown in red). Nuclear DNA was labeled with DAPI (shown in blue) and GFP is shown in green.
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