JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB308533

Alexa Fluor® 647 Anti-Adenosine Receptor A2a antibody [EPR23731-50]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal Adenosine Receptor A2a antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-P and reacts with Mouse, Human, Rat samples.

View Alternative Names

ADORA2, ADORA2A, Adenosine receptor A2a

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Adenosine Receptor A2a antibody [EPR23731-50] (AB308533)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Adenosine Receptor A2a antibody [EPR23731-50] (AB308533)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Adenosine Receptor A2a with ab308533 at 1/100 (5.0 ug/ml). Positive staining on mouse cerebrum. The section was incubated with ab308533 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Adenosine Receptor A2a antibody [EPR23731-50] (AB308533)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Adenosine Receptor A2a antibody [EPR23731-50] (AB308533)

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling Adenosine Receptor A2a with ab308533 at 1/100 (5.0 ug/ml). Negative control : no staining on mouse skeletal muscle. The section was incubated with ab308533 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

  • Carrier free

    Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free

  • Unconjugated

    Anti-Adenosine Receptor A2a antibody [EPR23731-50]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23731-50

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Mouse, Human, Mouse, Rat

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Biochemicals

AB141257

5'-Deoxy-5'-methylthioadenosine (Methylthioadenosine), Adenosine A1 receptor agonist

Chemical Structure - 5'-Deoxy-5'-methylthioadenosine (Methylthioadenosine), Adenosine A1 receptor agonist (AB141257)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Adenosine Receptor A2A also known as A2AR is a G protein-coupled receptor with a molecular mass of approximately 45 kDa. This receptor is widely expressed in various body tissues including the brain heart spleen and immune cells. A2A receptors play a mechanical role in mediating physiological responses to adenosine a purine nucleoside that acts as a signaling molecule in the body. Through binding to adenosine A2A receptors activate intracellular pathways that modulate cellular responses.
Biological function summary

The A2A adenosine receptor functions as an important regulator of neurotransmission and immune response. It does not form part of a larger protein complex but rather independently influences processes such as vasodilation heart rate regulation and cytokine release. The A2A receptor contributes to the modulation of dopamine and glutamate signaling in the central nervous system playing a significant role in maintaining neural balance. Its activity in immune cells helps in controlling inflammation and immune cell proliferation.

Pathways

The A2A adenosine receptor engages in critical signaling cascades particularly the cAMP-PKA pathway and the MAPK pathway. Through the cAMP-PKA pathway A2A R influences various cellular processes by increasing cyclic AMP levels which activate protein kinase A. The receptor is also involved in the MAPK pathway impacting cellular growth and differentiation. It interacts with proteins such as Gs alpha subunit which couples to the receptor to promote downstream signaling events.

The A2A receptor has connections to conditions like Parkinson's disease and ischemia-reperfusion injury. In Parkinson's disease the activity of A2A receptors affects dopaminergic signaling where it interacts with proteins such as dopamine D2 receptors suggesting potential for therapeutic targeting. During ischemia-reperfusion injury the receptor's modulation of immune responses and vasodilation can influence tissue damage and repair mechanisms linking it with proteins involved in inflammatory pathways.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Receptor for adenosine (By similarity). The activity of this receptor is mediated by G proteins which activate adenylyl cyclase (By similarity).
See full target information ADORA2A
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com