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AB309987

Alexa Fluor® 647 Anti-AICDA antibody [EPR23436-45] - ChIP Grade

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Rabbit Recombinant Monoclonal AICDA antibody - conjugated to Alexa Fluor® 647.

View Alternative Names

AID, AICDA, Single-stranded DNA cytosine deaminase, Activation-induced cytidine deaminase, Cytidine aminohydrolase

  • 578 PE

    PE Anti-AICDA antibody [EPR23436-45] (ChIP Grade)

  • 660 APC

    APC Anti-AICDA antibody [EPR23436-45] (ChIP Grade)

  • HRP

    HRP Anti-AICDA antibody [EPR23436-45] (ChIP Grade)

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-AICDA antibody [EPR23436-45] - ChIP Grade

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-AICDA antibody [EPR23436-45] - ChIP Grade

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-AICDA antibody [EPR23436-45] - ChIP Grade

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-AICDA antibody [EPR23436-45] - ChIP Grade

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-AICDA antibody [EPR23436-45] - ChIP Grade

  • Unconjugated

    Anti-AICDA antibody [EPR23436-45] - ChIP Grade

  • Carrier free

    Anti-AICDA antibody [EPR23436-45] - ChIP Grade - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23436-45

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Applications

Antibody Labelling, Target Binding Affinity

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AICDA also known as activation-induced cytidine deaminase is an enzyme with a molecular mass of approximately 25 kDa. It is expressed in germinal center B cells within the lymphoid tissues particularly in the spleen and lymph nodes. AICDA catalyzes the deamination of cytidine to uridine in single-stranded DNA an essential step in the processes of somatic hypermutation and class switch recombination. These mechanisms are important for antibody diversity and specificity in the adaptive immune response.
Biological function summary

AICDA plays an important role in the adaptive immune system by facilitating the diversification of antibodies. It does not appear to function as part of a larger protein complex but operates independently to induce mutations in variable regions of immunoglobulin genes. This activity enables B cells to produce antibodies with higher affinity and different isotypes important for an effective immune defense system. AICDA’s function ensures the generation of diverse antibody repertoires allowing the immune system to effectively target a wide range of pathogens.

Pathways

AICDA is integral to the immunoglobulin gene diversification pathway. It initiates somatic hypermutation and class switch recombination by deaminating cytosine residues in the DNA of immunoglobulin genes. This action relates AICDA to other proteins involved in DNA repair and error-prone replication such as uracil-DNA glycosylase (UNG) and the mismatch repair proteins like MSH2 and MSH6. AICDA's deamination activity triggers the recruitment of these proteins to introduce mutations and remove uracil residues facilitating the generation of antibody diversity.

Aberrant AICDA activity associates with lymphomas and autoimmune conditions. For example dysregulation of AICDA could contribute to oncogenic mutations leading to non-Hodgkin's lymphoma. Additionally incorrect or unscheduled activation of AICDA might result in the production of autoantibodies implicated in autoimmune disorders like systemic lupus erythematosus (SLE). AICDA’s interaction with DNA repair proteins during misguided activation may lead to genomic instability linking it to both tumorigenesis and autoimmunity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Single-stranded DNA-specific cytidine deaminase. Involved in somatic hypermutation (SHM), gene conversion, and class-switch recombination (CSR) in B-lymphocytes by deaminating C to U during transcription of Ig-variable (V) and Ig-switch (S) region DNA. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses (PubMed : 18722174, PubMed : 21385873, PubMed : 21518874, PubMed : 27716525). May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation (PubMed : 21496894).
See full target information AICDA
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