AB196919

Alexa Fluor® 647 Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184]

Name: Alexa Fluor® 647 Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184] (ab196919) | Abcam
Brand: Abcam Limited
SKU: AB196919
Rating: 5 (6 reviews)

RabMAb
Recombinant
What is this?

(6 Reviews)

(6 Publications)

Rabbit Recombinant Monoclonal alpha smooth muscle Actin acetyl E3 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 6 publications.

View Alternative Names

ACTSA, ACTVS, GIG46, ACTA2, Alpha-actin-2, Cell growth-inhibiting gene 46 protein, ACTA3, ACTL3, ACTSG, ACTG2, Alpha-actin-3, Gamma-2-actin, Smooth muscle gamma-actin

1 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184] (AB196919)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184] (AB196919)

ab196919 staining alpha Smooth Muscle Actin in HeLa cells. The cells were fixed with 4% PFA (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196919 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at 2μg/ml (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

519 FITC

FITC Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184]
578 PE

PE Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184]
421 Alexa Fluor® 405

Alexa Fluor® 405 Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184]
HRP

HRP Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184]
660 APC

APC Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184]
Unconjugated

Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184]
Carrier free

Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

E184

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

Reacts with

Human, Human, Mouse, Rat

Applications

Flow Cyt (Intra), IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The antibody has been shown to detect P62736-ACTA (gene ACTA2): (Ac)-EEEDSTALVC and P63267-ACTH (gene ACTG2): (Ac)-EEETTALVC in indirect ELISA.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha smooth muscle Actin also known as ACTG2 is an important protein in muscle contraction and cellular structure. It is most commonly found in smooth muscle cells and is not typically expressed in striated muscle. This protein is part of the actin family and has a molecular mass of approximately 42 kDa. ACTG2 plays a role in forming microfilaments which are key components of the muscle contractile machinery. In smooth muscle tissues such as those found in the intestines and blood vessels ACTG2 is often detected together with other cytoskeletal and regulatory proteins.

Biological function summary

ACTG2 contributes to muscle contraction by facilitating the interaction between actin and myosin. It is not considered a part of a protein complex but interacts closely with several other proteins involved in contractile functions. Beyond contraction ACTG2 also supports cellular integrity and shape maintenance. In tissues ACTG2 is involved in activities that require dynamic structural reorganization such as during cellular migration and wound healing highlighting its versatility beyond just a contractile agent.

Pathways

ACTG2 participates in the regulation of the smooth muscle contraction pathway influencing muscle tone and responsiveness to stimuli. The actin-myosin interaction where ACTG2 is involved is critical for the contractile process with other proteins like myosin light chain kinase (MLCK) and tropomyosin playing supportive roles. Additionally the integrin signaling pathway is another context where ACTG2 is indirectly involved aiding in the transmission of mechanical signals from the extracellular matrix to the cell.

ACTG2 is associated with conditions such as megacystis-microcolon-intestinal hypoperistalsis syndrome (MMIHS) and various vascular disorders. Mutations in the ACTG2 gene can disrupt normal muscle function leading to defective peristalsis in the gastrointestinal tract as seen in MMIHS. The connection between ACTG2 and myosin regulatory light chains illustrates how changes in its function can impact muscle contractility and lead to disease. In vascular disorders abnormal expression of ACTG2 is often linked to impaired vessel tone and blood flow regulation.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

See full target information ACTA2 acetyl E3

style

left-column

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Polymers 15: PubMed37376295

2023

Collagen Scaffolds Laden with Human Periodontal Ligament Fibroblasts Promote Periodontal Regeneration in SD Rat Model.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Tao Chang,Chuan-Ching Lai,Dan-Jae Lin

Scientific reports 10:17914 PubMed33087819

2020

TGF-β1 is a regulator of the pyruvate dehydrogenase complex in fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Edward R Smith,Timothy D Hewitson

EBioMedicine 55:102694 PubMed32335370

2020

Long non-coding RNA CDKN2B-AS1 contributes to atherosclerotic plaque formation by forming RNA-DNA triplex in the CDKN2B promoter.

Applications

Unspecified application

Species

Unspecified reactive species

Minghui Ou,Xia Li,Shibo Zhao,Shichao Cui,Jie Tu

Cell host & microbe 24:833-846.e6 PubMed30543778

2018

Microbiota-Derived Lactate Accelerates Intestinal Stem-Cell-Mediated Epithelial Development.

Applications

Unspecified application

Species

Unspecified reactive species

Yong-Soo Lee,Tae-Young Kim,Yeji Kim,Su-Hyun Lee,Seungil Kim,Sung Wan Kang,Jin-Young Yang,In-Jeoung Baek,Young Hoon Sung,Yun-Yong Park,Sung Wook Hwang,Eunju O,Kwang Soon Kim,Siqing Liu,Nobuhiko Kamada,Nan Gao,Mi-Na Kweon

Oncogenesis 6:e375 PubMed28846080

2017

Growth-induced stress enhances epithelial-mesenchymal transition induced by IL-6 in clear cell renal cell carcinoma via the Akt/GSK-3β/β-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Q Chen,D Yang,H Zong,L Zhu,L Wang,X Wang,X Zhu,X Song,J Wang

Scientific reports 7:9269 PubMed28839164

2017

CUBIC pathology: three-dimensional imaging for pathological diagnosis.

Applications

Unspecified application

Species

Unspecified reactive species

Satoshi Nojima,Etsuo A Susaki,Kyotaro Yoshida,Hiroyoshi Takemoto,Naoto Tsujimura,Shohei Iijima,Ko Takachi,Yujiro Nakahara,Shinichiro Tahara,Kenji Ohshima,Masako Kurashige,Yumiko Hori,Naoki Wada,Jun-Ichiro Ikeda,Atsushi Kumanogoh,Eiichi Morii,Hiroki R Ueda

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 647 Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184] (AB196919)

Related conjugates and formulations (7)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise