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AB202296

Alexa Fluor® 647 Anti-alpha smooth muscle Actin antibody [EPR5368]

4

(6 Reviews)

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(10 Publications)

Anti-alpha smooth muscle Actin antibody [EPR5368] - Alexa Fluor® 647 conjugated (ab202296) is a rabbit recombinant monoclonal antibody detecting alpha smooth muscle Actin in ICC/IF. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

ACTSA, ACTVS, GIG46, ACTA2, Alpha-actin-2, Cell growth-inhibiting gene 46 protein

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-alpha smooth muscle Actin antibody [EPR5368] (AB202296)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-alpha smooth muscle Actin antibody [EPR5368] (AB202296)

ab202296 staining alpha smooth muscle Actin in SV40LT-SMC cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab202296 at 1/5000 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in SV40LT-SMC cells fixed with 100% methanol (5min).

  • Carrier free

    Anti-alpha smooth muscle Actin antibody [EPR5368] – Goat IgG (Chimeric) – BSA and Azide Free

  • Carrier free

    Anti-alpha smooth muscle Actin antibody [EPR5368] - BSA and Azide free

  • Unconjugated

    Anti-alpha smooth muscle Actin antibody [EPR5368]

  • 660 APC

    APC Anti-alpha smooth muscle Actin antibody [EPR5368]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [EPR5368]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-alpha smooth muscle Actin antibody [EPR5368]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-alpha smooth muscle Actin antibody [EPR5368]

  • 578 PE

    PE Anti-alpha smooth muscle Actin antibody [EPR5368]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5368

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human, Human, Mouse, Rat

Applications

IHC-P, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

Primary Antibodies

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Secondary Antibodies

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Primary Antibodies

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Primary Antibodies

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AB194719

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/5000", "ICCIF-species-notes": "<p>This product gave a positive signal in SV40LT-SMC cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).</p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

What is this antibody validated in?
Alexa Fluor® 647 Anti-alpha smooth muscle Actin antibody [EPR5368] (ab202296) is a rabbit recombinant monoclonal antibody and is validated for use in Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

Reviewed by scientists
Anti-alpha smooth muscle Actin [EPR5368] Alexa Fluor® 647 (ab202296) has over 5 independent reviews from customers.

Related products
Antibody clone EPR5368 is also available pre-conjugated to a variety of labels for your convenience – Anti-alpha smooth muscle Actin Alexa Fluor® 647 [EPR5368] (ab202296).

Other related products
We have a range of other formats of antibody clone [EPR5368] also available for your convenience: ab124964, Alexa Fluor® 488 - ab202295, Alexa Fluor® 647 - ab202296, Alexa Fluor® 555 - ab202509, Alexa Fluor® 594 - ab202510, PE - ab208844, Carrier free - ab220795, APC - ab225143, ab284414, Alexa Fluor® 790 - ab309335, ab320058, Carrier free - ab320059, ab321866, Carrier free - ab322917

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha smooth muscle actin (α-SMA) also known as ACTA2 is an actin isoform with a specific role in the contractile function of smooth muscle cells. The molecular weight of α-SMA is approximately 42 kDa. This protein is expressed widely in vascular smooth muscle cells the peritoneal lining and myofibroblasts. It often serves as a marker for these cell types. The expression of α-SMA is critical for the mechanical attributes of cells contributing to the rigidity and contractility of tissues where it is present.
Biological function summary

Alpha smooth muscle actin aids in maintaining the structural integrity of tissues by forming part of the actin cytoskeleton an important element in cellular support. It exists in high concentration in stress fibers contributing to cellular movements and shape maintenance. These actions are essential in various dynamic cellular processes such as cell migration and adhesion. Alpha smooth muscle actin does not typically form complexes but it associates with other components of the actin cytoskeleton to ensure cell stability and function.

Pathways

Alpha smooth muscle actin functions significantly within the TGF-beta signaling pathway which influences cell proliferation differentiation and apoptosis. It interacts closely with proteins such as myosin to facilitate cellular contractility and motility. Additionally α-SMA plays a part in the RhoA/Rho kinase (ROCK) pathway connecting with regulators of actin filament organization. These pathways are essential for modulation of smooth muscle contraction and actin filament assembly contributing to vascular development and wound healing.

Alpha smooth muscle actin is importantly involved in fibrotic diseases and vascular diseases like arteriosclerosis. Increased expression of α-SMA is often observed in fibrotic tissues contributing to pathogenesis due to excessive extracellular matrix deposition. In the context of vascular disorders overexpression of α-SMA can lead to abnormal vascular remodeling often seen in diseases like hypertension. Interactions with proteins such as fibronectin and collagens facilitate these pathological changes highlighting the importance of α-SMA in disease development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
See full target information ACTA2
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:23770 PubMed40610502

2025

Discovering molecular signatures in kidney transplant biopsies with borderline changes and isolated V-lesions: single-cell RNA-sequencing analysis of human blood and tissue Spatial transcriptomics.

Applications

Unspecified application

Species

Unspecified reactive species

Jeongin Song,Minji Kang,Yunyoung Jang,Christine Suh-Yun Joh,Ha Yeon Shin,Julia Young Baik,Hyun Seung Choi,Kyu Hong Kim,Seong Min Lee,Seung-Min Cha,Hyunah Ku,Ahram Han,Sang Il Min,Jongwon Ha,Kyung Chul Moon,Sehoon Park,Seung Seok Han,Hajeong Lee,Dong Ki Kim,Kook Hwan Oh,Kwon Wook Joo,Ji Hwan Moon,Chung-Gyu Park,Yon Su Kim,Seung Hee Yang,Hyun Je Kim,Yong Chul Kim

International journal of molecular sciences 26: PubMed40244230

2025

Aldosterone-Induced Transformation of Vascular Smooth Muscle Cells into Macrophage-like Cells Participates in Inflammatory Vascular Lesions.

Applications

Unspecified application

Species

Unspecified reactive species

Boya Zhang,Ziqian Liu,Yi Chang,Ruyan Lv,Haixia Guo,Panpan Qiang,Tatsuo Shimosawa,Qingyou Xu,Fan Yang

Journal of nephrology 38:1819-1829 PubMed40067649

2025

Identification of conserved gene expression changes across common glomerular diseases by spatial transcriptomics.

Applications

Unspecified application

Species

Unspecified reactive species

Jeong Min Cho,Minji Kang,Sehoon Park,Jaeik Oh,Hyunah Ku,Ha Yeon Shin,Jung Hun Koh,Semin Cho,Yaerim Kim,Soojin Lee,Yong Chul Kim,Seung Seok Han,Kwon-Wook Joo,Yon Su Kim,Seung Hee Yang,Kyung Chul Moon,Hajeong Lee,Hyun Je Kim,Dong Ki Kim

Scientific reports 14:21581 PubMed39285184

2024

Application of testicular organ culture system for the evaluation of spermatogenesis impairment.

Applications

Unspecified application

Species

Unspecified reactive species

Hideaki Nakagiri,Takehiko Ogawa,Naohiro Ikeda,Shimpei Terasaka,Yuko Nukada,Masaaki Miyazawa

Cells 13: PubMed39195275

2024

Cell-Specific Effects of Insulin in a Murine Model of Restenosis Under Insulin-Sensitive and Insulin-Resistant Conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Marel Gonzalez Medina,Zhiwei Liu,Johny Wang,Cindy Zhang,Sarah B Cash,Carolyn L Cummins,Adria Giacca

Scientific reports 14:9976 PubMed38693148

2024

Eplerenone reduces lymphangiogenesis in the contralateral kidneys of UUO rats.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Hao,Panpan Qiang,Lili Fan,Yunzhao Xiong,Yi Chang,Fan Yang,Xiangting Wang,Tatsuo Shimosawa,Shengyu Mu,Qingyou Xu

Scientific reports 14:2211 PubMed38278877

2024

Glomerular spatial transcriptomics of IgA nephropathy according to the presence of mesangial proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Sehoon Park,Minji Kang,Yong Chul Kim,Dong Ki Kim,Kook-Hwan Oh,Kwon Wook Joo,Yon Su Kim,Hyun Je Kim,Kyung Chul Moon,Hajeong Lee

Cell 186:5910-5924.e17 PubMed38070509

2023

A tridimensional atlas of the developing human head.

Applications

IHC - Wmt

Species

Human

Raphael Blain,Gérard Couly,Eimad Shotar,Joséphine Blévinal,Maryne Toupin,Anais Favre,Ali Abjaghou,Megumi Inoue,Edwin Hernández-Garzón,Frédéric Clarençon,Frédéric Chalmel,Séverine Mazaud-Guittot,Paolo Giacobini,Yorick Gitton,Alain Chédotal

International journal of molecular sciences 24: PubMed37511521

2023

Esaxerenone Inhibits Renal Angiogenesis and Endothelial-Mesenchymal Transition via the VEGFA and TGF-β1 Pathways in Aldosterone-Infused Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaomeng Gao,Jingyue Chang,Yi Chang,Lili Fan,Ziqian Liu,Cuijuan Zhang,Tatsuo Shimosawa,Fan Yang,Qingyou Xu

Frontiers in immunology 13:948658 PubMed36148244

2022

Esaxerenone inhibits the macrophage-to-myofibroblast transition through mineralocorticoid receptor/TGF-β1 pathway in mice induced with aldosterone.

Applications

Unspecified application

Species

Unspecified reactive species

Panpan Qiang,Juan Hao,Fan Yang,Yutong Han,Yi Chang,Yunqian Xian,Yunzhao Xiong,Xiaomeng Gao,Lijuan Liang,Tatsuo Shimosawa,Qingyou Xu
View all publications
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