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AB307612

Alexa Fluor® 647 Anti-Alpha-synuclein, isoform 98 and 112 antibody [EPR25115-65]

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Rabbit Recombinant Monoclonal Alpha-synuclein antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF, IHC-P and reacts with Transfected cell line samples.

View Alternative Names

NACP, PARK1, SNCA, Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor

2 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Alpha-synuclein, isoform 98 and 112 antibody [EPR25115-65] (AB307612)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Alpha-synuclein, isoform 98 and 112 antibody [EPR25115-65] (AB307612)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Alpha-synuclein, isoform 98 and 112 with ab307612 at 1/500 (1.0 μg/ml) dilution. Confocal image showing cytoplasmic and nuclear staining in HEK-293T cells transfected with a human SNCA112 expression vector and a human SNCA98 expression vector containing a myc tag, and no staining in HEK-293T cells transfected with a human SNCA126 expression vector or a human SNCA140 expression vector containing a myc tag. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Myc-Tag Mouse mAb (Alexa Fluor® 488) was used to counterstain tubulin at 1/100 0.5 μg/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Alpha-synuclein, isoform 98 and 112 antibody [EPR25115-65] (AB307612)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Alpha-synuclein, isoform 98 and 112 antibody [EPR25115-65] (AB307612)

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T cells t tissue labeling Alpha-synuclein, isoform 98 and 112 with ab307612 at 1/100 (5.0 μg/ml). Posiitive staining on (A) HEK-293T cells transfected with a SNCA112 expression vector and (B) HEK-293T cells transfected with a SNCA98 expression vector, no staining on (C) HEK-293T cells transfected with a SNCA140 expression vector and (D) HEK-293T cells transfected with a SNCA126 expression vector. The section was incubated with ab307612 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

  • Carrier free

    Anti-Alpha-synuclein, isoform 98 and 112 antibody [EPR25115-65] - BSA and Azide free

  • Unconjugated

    Anti-Alpha-synuclein, isoform 98 and 112 antibody [EPR25115-65]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25115-65

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Applications

ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Transfected cell line": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha-synuclein also known as alpha-synuclein kDa or isoform ANX-112 is a protein with a molecular mass of approximately 14 kDa. It is mostly expressed in the brain especially in the presynaptic terminals of neurons. Structurally it is a small cytosolic protein that can form amyloid fibrils and is sensitive to chemical modifications. Alpha-synuclein plays a role in the regulation of neurotransmitter release and neuronal plasticity though its precise mechanisms remain an area of active investigation.
Biological function summary

Alpha-synuclein interacts with various membrane phospholipids and can form insoluble aggregates. These aggregates are part of complex structures known as Lewy bodies prominent in neurodegenerative conditions. This protein is implicated in synaptic vesicle trafficking maintaining synaptic function and neuronal adaptive responses. Alpha-synuclein may also modulate enzyme activities and participate in genomic stability reflecting its versatile cellular roles.

Pathways

Alpha-synuclein is involved in the dopamine synthesis pathway and membrane trafficking pathways. In these pathways it interacts with proteins like synapsin and SNAREs influencing vesicle fusion and neurotransmitter packaging. Its association with such pathways highlights its role in maintaining neuronal integrity and communication. Dysregulation of alpha-synuclein's normal function might disrupt these pathways resulting in pathological consequences.

Alpha-synuclein is most commonly linked to Parkinson's disease and Lewy body dementia. In these conditions abnormal accumulation and aggregation of alpha-synuclein into Lewy bodies occur. These aggregates impede cellular function and promote neuronal death. Additionally its relationship with other proteins like tau in the neurodegenerative disease context highlights its significant impact in multiple disorders associated with cellular aging and synaptic dysfunction.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release (PubMed : 20798282, PubMed : 26442590, PubMed : 28288128, PubMed : 30404828). Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores (PubMed : 28288128, PubMed : 30404828). Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis (PubMed : 30404828). Also acts as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5 (PubMed : 20798282). This chaperone activity is important to sustain normal SNARE-complex assembly during aging (PubMed : 20798282). Also plays a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity (PubMed : 26442590).
See full target information SNCA
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com