AB218591

Alexa Fluor® 647 Anti-alpha Tubulin (acetyl K40) antibody [EPR16772]

RabMAb
Recombinant
What is this?

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(6 Publications)

Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] - Alexa Fluor® 647 conjugated (ab218591) is a rabbit recombinant monoclonal antibody detecting alpha Tubulin in ICC/IF. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

TUBA1, TUBA4A, Tubulin alpha-4A chain, Alpha-tubulin 1, Testis-specific alpha-tubulin, Tubulin H2-alpha, Tubulin alpha-1 chain

1 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (AB218591)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (AB218591)

ab218591 staining alpha Tubulin (acetyl K40) in HeLa cells +/- Trichostatin A (500ng/ml, 4 hours). The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h.

The cells were then incubated overnight at +4°C with ab218591 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

578 PE

PE Anti-alpha Tubulin (acetyl K40) antibody [EPR16772]
660 APC

APC Anti-alpha Tubulin (acetyl K40) antibody [EPR16772]
HRP

HRP Anti-alpha Tubulin (acetyl K40) antibody [EPR16772]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-alpha Tubulin (acetyl K40) antibody [EPR16772]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-alpha Tubulin (acetyl K40) antibody [EPR16772]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-alpha Tubulin (acetyl K40) antibody [EPR16772]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-alpha Tubulin (acetyl K40) antibody [EPR16772]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-alpha Tubulin (acetyl K40) antibody [EPR16772]
Unconjugated

Anti-alpha Tubulin (acetyl K40) antibody [EPR16772]
Carrier free

Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16772

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in HeLa cells treated with Trichostatin A (500ng/ml, 4 hours) fixed with 4% formaldehyde (10 min)</p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

What is this antibody validated in?
Alexa Fluor® 647 Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab218591) is a rabbit recombinant monoclonal antibody and is validated for use in Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

Related products
Antibody clone EPR16772 is also available pre-conjugated to a variety of labels for your convenience – Anti-alpha Tubulin Alexa Fluor® 647 (acetyl K40) [EPR16772] (ab218591).

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha tubulin also known as DM1A is an important component of the structural unit called microtubules. This protein weighs approximately 50 kDa and acts as a building block in the cytoskeleton. Alpha tubulin is expressed in various cell types across many organisms playing a fundamental role in maintaining cell shape and enabling intracellular transport. Researchers often acetylate alpha tubulin to study post-translational modifications and its effects on microtubule dynamics.

Biological function summary

Alpha tubulin combines with beta tubulin to form the tubulin dimer which is the basic unit of microtubule polymerization. These dimers assemble into microtubule filaments integral in numerous cellular processes. As part of the cytoskeleton network alpha tubulin facilitates roles in mitosis and meiosis providing spindle structures necessary for chromosome separation. Its participation in cell signaling pathways is noteworthy as it interacts with motor proteins like kinesins and dyneins to aid cellular activities.

Pathways

Alpha tubulin holds critical roles in microtubule-associated processes within the cytoskeleton and intracellular transport pathways. It interacts with the MAP (microtubule-associated protein) family which modulates microtubule stability and impacts cell division and trafficking. Moreover the microtubule pathway involves proteins like tau which bind to stabilize microtubules and are implicated in cellular dynamics and signaling.

Alpha tubulin is relevant in neurodegenerative diseases such as Alzheimer's disease and cancer. Aberrant modifications or mutations in alpha tubulin or associated proteins like tau can disrupt microtubule stability contributing to pathological conditions. In cancer altered expression of alpha tubulin affects cell division leading to tumor progression and chemoresistance. Researchers study alpha tubulin using techniques such as immunofluorescence with Alexa fluor 555 to understand its role in disease better.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

See full target information TUBA4A acetyl K40

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Cell communication and signaling : CCS 23:417 PubMed41044645

2025

Tubacin alleviate the reproductive toxicity of deoxynivalenol in mouse oocytes and zygotes via strengthening microtubule stability.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Luo,Jianhua Chen,Zhihan Guo,Yanyan Zhu,Yipin Wang,Tian Wu,Siyue Yin,Cao Li,Youqiang Su,Yao Chen,Yun Qian,Congxiu Miao,Ruizhi Feng

ACS applied bio materials 8:2875-2887 PubMed40130574

2025

Microstructural Effects of Melt Electrowritten-Reinforced Hydrogel Scaffolds for Engineering Thick Skin Substitutes.

Applications

Unspecified application

Species

Unspecified reactive species

Ferdows Afghah,Mine Altunbek,Mahdiyeh Zahrabi,Bahattin Koc

Clinical and translational medicine 15:e70193 PubMed39834092

2025

Pathogenic variants of TUBB8 cause oocyte spindle defects by disrupting with EB1/CAKP5 interactions and potential treatment targeting microtubule acetylation through HDAC6 inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Luo,Jianhua Chen,Cao Li,Tian Wu,Siyue Yin,Guangping Yang,Yipin Wang,Zhihan Guo,Saifei Hu,Yanni He,Yingnan Wang,Yao Chen,Youqiang Su,Congxiu Miao,Yun Qian,Ruizhi Feng

Respiratory research 25:102 PubMed38419061

2024

P80 natural essence spray and lozenges provide respiratory protection against Influenza A, B, and SARS-CoV-2.

Applications

Unspecified application

Species

Unspecified reactive species

Viktoria Zaderer,Gabriel Diem,Wilfried Posch,Thomas Jakschitz,Günther K Bonn,Rosa Bellmann-Weiler,Lukas A Huber,Doris Wilflingseder

Structure (London, England : 1993) 31:1233-1246.e5 PubMed37572662

2023

Structural basis of microtubule depolymerization by the kinesin-like activity of HIV-1 Rev.

Applications

Unspecified application

Species

Unspecified reactive species

Elif Eren,Norman R Watts,Davide Randazzo,Ira Palmer,Dan L Sackett,Paul T Wingfield

Respiratory research 24:88 PubMed36949547

2023

GlyPerA™ effectively shields airway epithelia from SARS-CoV-2 infection and inflammatory events.

Applications

Unspecified application

Species

Unspecified reactive species

Viktoria Zaderer,Stefanie Dichtl,Wilfried Posch,Ivane Abiatari,Günther K Bonn,Thomas Jakschitz,Lukas A Huber,Teymuras V Kurzchalia,Doris Wilflingseder

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 647 Anti-alpha Tubulin (acetyl K40) antibody [EPR16772]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (AB218591)

Related conjugates and formulations (10)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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Product promise