Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker

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Immunocytochemistry - Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (AB190573)

ab277270 staining pan Cytokeratin cells. The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab277270 at at 1µg/ml (shown in green) and ab190573, Rabbit monoclonal [EP1332Y] to alpha Tubulin 4 - Microtubule Marker (Alexa Fluor^® 647), pre-adsorbed at 1/250 dilution (shown in magenta) dilution, overnight at 4°C. Nuclear DNA was labelled in blue with DAPI (shown in blue).

Also suitable in cells fixed with 4% paraformaldehyde (10 minutes).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (AB190573)

Immunofluorescent analysis of HeLa (human cervical cancer) cells, fixed with 100% methanol (5 min). The cells were permeabilized with 0.1% Triton X-100 for 5 minutes and blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab209478 (Rabbit IgG, monoclonal [EPR25A] - Isotype Control) at 1/100 dilution (showing no signal) and ab190573, Rabbit monoclonal to Tubulin Microtubule Marker (Alexa Fluor^® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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Immunocytochemistry - Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (AB190573)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (AB190573)

ab197235 staining FOXA1 in HepG2 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab197235 at a 1/100 dilution (shown in green) and ab190573, Rabbit monoclonal to alpha-Tubulin (Alexa Fluor^® 647), at a 1/250 dilution (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (AB190573)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat IDH2 knockout cells labeling IDH2 with ab212122 (green) at 1 μg/ml. Cells were counterstained with ab190573 Anti-Tubulin (Alexa Fluor® 647) at 1 : 250 (2 μg/ml) (magenta). The nuclear counterstain was DAPI (blue).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (AB190573)

ab190573 staining alpha Tubulin in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab190573 at 1/500 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (AB190573)

ab190573 staining alpha Tubulin in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab190573 at a working dilution of 1 in 100 (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor^® 488, shown in green) at 2μg/ml overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

This product also gave a positive signal in 100% methanol (5 min) fixed HeLa cells under the same testing conditions.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (AB190573)

ab1220 staining Histone H3 (di methyl K9) in HeLa (Human cervix adenocarcinoma epithelial cell) cells. The cells were fixed with 4% PFA (10 mins), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab1220 at 5 µg/ml and ab190573, Rabbit monoclonal to alpha Tubulin (Alexa Fluor® 647), at 2 µg/ml (shown in red). The secondary antibody (shown in green) was ab150117, Alexa Fluor® 488 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1h at room temperature. Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown. This product also gave a positive outcome under the same testing conditions in HeLa cells fixed with 100% methanol (5 mins).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (AB190573)

Immunofluorescent analysis of 4% PFA-fixed 0.1% Triton X-100 permeabilized A549 WT and A549-SLC2A1 KO cells labelling Glucose Transporter GLUT1 with ab209449 at 1 μg/ml concentration (Green). Cells were counterstained with ab190573 Anti-Tubulin (Alexa Fluor® 647) at 1 : 250 (2 μg/ml) (Magenta). The nuclear counterstain was DAPI (Blue).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (AB190573)

Immunofluorescent analysis of 4% PFA-fixed 0.1% Triton X-100 permeabilized HEK-293 WT and HEK-293-HK1 KO cells labelling Hexokinase 1 with ab184818 at 0.2 μg/ml concentration (Green). Cells were counterstained with ab190573 Anti-Tubulin (Alexa Fluor® 647) at 1 : 250 (2 μg/ml) (Magenta). The nuclear counterstain was DAPI (Blue).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (AB190573)

ab314401 staining PBR in Hela cells, with negative expression in Hela-TSPO KO cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised with 0.1% Triton x-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab314401 at 0.2 μg/ml (shown in green). ab190573, rabbit monoclonal to alpha Tubulin (Alexa Fluor® 647) antibody (shown in red) was used at 5 μg/ml for structural counterstaining. Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown. This product also gave a positive signal in 100% methanol (5 min) fixed HeLa cells and no signal Hela-TSPO KO under the same testing conditions.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (AB190573)

Immunofluorescent analysis of 100% methanol-fixed 0.1% Triton X-100 permeabilized Hap1 WT and Hap1-PRDX2 KO cells labelling Peroxiredoxin 2 with ab197536 (green) at 1 μg/ml. Cells were counterstained with ab190573 Anti-Tubulin (Alexa Fluor® 647) at 1 : 250 (2 μg/ml) (magenta). The nuclear counterstain was DAPI (Blue).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (AB190573)

ab281778 staining LAMP1 in HAP1 cells, with negative expression in LAMP1 knockout HAP1 cells. The cells were fixed with 100% methanol (5 min), permeabilised with 0.1% Triton x-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab281778 at 0.2 μg/ml (shown in green). ab190573, rabbit monoclonal to alpha Tubulin (Alexa Fluor^® 647) (shown in magenta) was used at 5 μg/ml for structural counterstaining.

Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

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Immunocytochemistry - Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (AB190573)

ab202295 staining alpha smooth muscle Actin (ACTA2) in wild-type NIH/3T3 cells (top panel) and ACTA2 knockout NIH/3T3 cells (bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab202295 at at 5µg/ml (shown in green) and ab190573, Rabbit monoclonal [EP1332Y] to alpha Tubulin 4 - Microtubule Marker (Alexa Fluor^® 647), pre-adsorbed at 1/250 dilution (shown in magenta) dilution, overnight at 4°C. Nuclear DNA was labelled in blue with DAPI (shown in blue).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

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Immunocytochemistry - Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (AB190573)

ab202509 staining alpha smooth muscle Actin (ACTA2) in wild-type NIH/3T3 cells (top panel) and ACTA2 knockout NIH/3T3 cells (bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab202509 at at 5µg/ml (shown in magenta) and ab190573, Rabbit monoclonal [EP1332Y] to alpha Tubulin 4 - Microtubule Marker (Alexa Fluor^® 647), pre-adsorbed at 1/250 dilution (shown in green) dilution, overnight at 4°C. Nuclear DNA was labelled in blue with DAPI (shown in blue).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

• ICC

Lab

Immunocytochemistry - Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (AB190573)

ab202295 staining staining alpha smooth muscle Actin (ACTA2) in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab202295 at 5µg/ml (shown in green) and ab190573, Rabbit monoclonal [EP1332Y] to alpha Tubulin 4 - Microtubule Marker (Alexa Fluor^® 647), pre-adsorbed at 1/250 dilution (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue).

Also suitable in cells fixed with 100% methanol (5 min).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (AB190573)

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (AB190573)

ab309685 staining MDK in SH-SY5Y (positive) and HeLa (negative) cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated for 2 hours at room temperature with ab309685 at 0.2 ug/ml (shown in green) and ab190573, Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (shown in pseudocolour magenta) at 1 in 1000 dilution. Nuclear DNA was labeled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Revvity) and a maximum intensity projection of confocal sections is shown.