Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker

12 product images

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab190573)

  ab190573 staining alpha Tubulin in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab190573 at 1/500 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
  

  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab190573)

  ab190573 staining alpha Tubulin in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab190573 at a working dilution of 1 in 100 (shown in red) and Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor^® 488, shown in green) at 2μg/ml overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

  This product also gave a positive signal in 100% methanol (5 min) fixed HeLa cells under the same testing conditions.

  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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  Immunocytochemistry - Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab190573)

  Alpha Tubulin Immunocytochemistry staining using rabbit Anti-alpha Tubulin antibody

  Alexa Fluor® 555 Anti-alpha smooth muscle Actin antibody [EPR5368] ab202509 staining alpha smooth muscle Actin (ACTA2) in wild-type NIH/3T3 cells (top panel) and ACTA2 knockout NIH/3T3 cells (bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with Alexa Fluor® 555 Anti-alpha smooth muscle Actin antibody [EPR5368] ab202509 at at 5µg/ml (shown in magenta) and ab190573, Rabbit monoclonal [EP1332Y] to alpha Tubulin 4 - Microtubule Marker (Alexa Fluor^® 647), pre-adsorbed at 1/250 dilution (shown in green) dilution, overnight at 4°C. Nuclear DNA was labelled in blue with DAPI (shown in blue).

  Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

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  Immunocytochemistry - Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab190573)

  Alpha Tubulin Immunocytochemistry staining using rabbit Anti-alpha Tubulin antibody

  Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [EPR5368] ab202295 staining alpha smooth muscle Actin (ACTA2) in wild-type NIH/3T3 cells (top panel) and ACTA2 knockout NIH/3T3 cells (bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [EPR5368] ab202295 at at 5µg/ml (shown in green) and ab190573, Rabbit monoclonal [EP1332Y] to alpha Tubulin 4 - Microtubule Marker (Alexa Fluor^® 647), pre-adsorbed at 1/250 dilution (shown in magenta) dilution, overnight at 4°C. Nuclear DNA was labelled in blue with DAPI (shown in blue).

  Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

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  Immunocytochemistry - Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab190573)

  Alpha Tubulin Immunocytochemistry staining of A431 (Human pan cytokeratin) cells using rabbit Anti-alpha Tubulin antibody

  Alexa Fluor® 488 Anti-pan Cytokeratin antibody [C-11] ab277270 staining pan Cytokeratin cells. The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with Alexa Fluor® 488 Anti-pan Cytokeratin antibody [C-11] ab277270 at at 1µg/ml (shown in green) and ab190573, Rabbit monoclonal [EP1332Y] to alpha Tubulin 4 - Microtubule Marker (Alexa Fluor^® 647), pre-adsorbed at 1/250 dilution (shown in magenta) dilution, overnight at 4°C. Nuclear DNA was labelled in blue with DAPI (shown in blue).

  Also suitable in cells fixed with 4% paraformaldehyde (10 minutes).

  Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

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  Immunocytochemistry - Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab190573)
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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab190573)

  Alexa Fluor® 488 Anti-FOXA1 antibody [EPR10881] ab197235 staining FOXA1 in HepG2 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with Alexa Fluor® 488 Anti-FOXA1 antibody [EPR10881] ab197235 at a 1/100 dilution (shown in green) and ab190573, Rabbit monoclonal to alpha-Tubulin (Alexa Fluor^® 647), at a 1/250 dilution (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue).

  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab190573)
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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab190573)

  Alexa Fluor® 568 Anti-PBR antibody [EPR5384] ab314401 staining PBR in Hela cells, with negative expression in Hela-TSPO KO cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised with 0.1% Triton x-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with Alexa Fluor® 568 Anti-PBR antibody [EPR5384] ab314401 at 0.2 μg/ml (shown in green). ab190573, rabbit monoclonal to alpha Tubulin (Alexa Fluor® 647) antibody (shown in red) was used at 5 μg/ml for structural counterstaining. Nuclear DNA was labelled with DAPI (shown in blue).
  Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
  This product also gave a positive signal in 100% methanol (5 min) fixed HeLa cells and no signal Hela-TSPO KO under the same testing conditions.

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab190573)

  Alpha Tubulin Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-alpha Tubulin antibody

  PE Anti-LAMP1 antibody [EPR24395-31] - Lysosome Marker ab281778 staining LAMP1 in HAP1 cells, with negative expression in LAMP1 knockout HAP1 cells. The cells were fixed with 100% methanol (5 min), permeabilised with 0.1% Triton x-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with PE Anti-LAMP1 antibody [EPR24395-31] - Lysosome Marker ab281778 at 0.2 μg/ml (shown in green). ab190573, rabbit monoclonal to alpha Tubulin (Alexa Fluor^® 647) (shown in magenta) was used at 5 μg/ml for structural counterstaining.

  Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab190573)

  Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade ab1220 staining Histone H3 (di methyl K9) in HeLa (Human cervix adenocarcinoma epithelial cell) cells. The cells were fixed with 4% PFA (10 mins), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade ab1220 at 5 µg/ml and ab190573, Rabbit monoclonal to alpha Tubulin (Alexa Fluor® 647), at 2 µg/ml (shown in red). The secondary antibody (shown in green) was Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117, Alexa Fluor® 488 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1h at room temperature. Nuclear DNA was labelled with DAPI (shown in blue).
  Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
  This product also gave a positive outcome under the same testing conditions in HeLa cells fixed with 100% methanol (5 mins).

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  Immunocytochemistry - Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab190573)

  Alpha Tubulin Immunocytochemistry staining of HeLa cells using rabbit Anti-alpha Tubulin antibody

  Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [EPR5368] ab202295 staining staining alpha smooth muscle Actin (ACTA2) in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [EPR5368] ab202295 at 5µg/ml (shown in green) and ab190573, Rabbit monoclonal [EP1332Y] to alpha Tubulin 4 - Microtubule Marker (Alexa Fluor^® 647), pre-adsorbed at 1/250 dilution (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue).

  Also suitable in cells fixed with 100% methanol (5 min).

  Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.