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Rabbit Monoclonal Annexin A1/ANXA1 antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-P and reacts with Human, Mouse, Rat samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Annexin A1/ANXA1 antibody [EPR19342] (AB309365), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Annexin A1/ANXA1 antibody [EPR19342] (AB309365), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Annexin A1/ANXA1 antibody [EPR19342] (AB309365), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Annexin A1/ANXA1 antibody [EPR19342] (AB309365), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Annexin A1/ANXA1 antibody [EPR19342] (AB309365), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-P
Human
Tested
Mouse
Tested
Rat
Tested

Tested
Tested

Species
Human
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

5 products for Alternative Version

Target data

Function

Plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. Has anti-inflammatory activity (PubMed:8425544). Plays a role in glucocorticoid-mediated down-regulation of the early phase of the inflammatory response (By similarity). Contributes to the adaptive immune response by enhancing signaling cascades that are triggered by T-cell activation, regulates differentiation and proliferation of activated T-cells (PubMed:17008549). Promotes the differentiation of T-cells into Th1 cells and negatively regulates differentiation into Th2 cells (PubMed:17008549). Has no effect on unstimulated T cells (PubMed:17008549). Negatively regulates hormone exocytosis via activation of the formyl peptide receptors and reorganization of the actin cytoskeleton (PubMed:19625660). Has high affinity for Ca(2+) and can bind up to eight Ca(2+) ions (By similarity). Displays Ca(2+)-dependent binding to phospholipid membranes (PubMed:2532504, PubMed:8557678). Plays a role in the formation of phagocytic cups and phagosomes. Plays a role in phagocytosis by mediating the Ca(2+)-dependent interaction between phagosomes and the actin cytoskeleton (By similarity). Annexin Ac2-26. Functions at least in part by activating the formyl peptide receptors and downstream signaling cascades (PubMed:15187149, PubMed:22879591, PubMed:25664854). Promotes chemotaxis of granulocytes and monocytes via activation of the formyl peptide receptors (PubMed:15187149). Promotes rearrangement of the actin cytoskeleton, cell polarization and cell migration (PubMed:15187149). Promotes resolution of inflammation and wound healing (PubMed:25664854). Acts via neutrophil N-formyl peptide receptors to enhance the release of CXCL2 (PubMed:22879591).

Alternative names

ANX1, LPC1, ANXA1, Annexin A1, Annexin I, Annexin-1, Calpactin II, Calpactin-2, Chromobindin-9, Lipocortin I, Phospholipase A2 inhibitory protein, p35

Recommended products

Rabbit Monoclonal Annexin A1/ANXA1 antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-P and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR19342
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Annexin A1 also known as ANXA1 or annexin 1 is a multifunctional protein with a molecular mass of approximately 37 kDa. This protein belongs to the annexin family and is distributed widely in various tissue types including the central nervous system respiratory system and immune cells. Annexin A1 functions by binding to phospholipids in a calcium-dependent manner influencing membrane-related processes and signal transduction. Its expression can be examined using techniques like annexin A1 immunohistochemistry and annexin A1 ELISA to study protein localization and concentration.

Biological function summary

Annexin A1 is involved in modulating inflammatory responses and cell proliferation. It acts as a mediator in the resolution of inflammation by promoting apoptotic cell clearance and regulating leukocyte migration. Annexin A1 participates in complex interactions often associating with cell membrane phospholipids to exert its effects. It contributes significantly to cell adhesion migration and intracellular signaling events which are important for maintaining homeostasis and proper immune function.

Pathways

Annexin A1 plays a significant role in the glucocorticoid signaling pathway and the formation of the cytoskeleton. It interacts with proteins like lipocortin 1 and phospholipase A2 impacting processes such as arachidonic acid metabolism and cytoskeletal reorganization. These interactions underline annexin A1's involvement in regulating inflammation and maintaining cellular architecture linking it to cellular processes that govern stress responses and cell movement.

Associated diseases and disorders

Annexin A1 exhibits relevance to inflammatory diseases and cancer. It has been implicated in conditions such as asthma where it modulates inflammatory cell recruitment and activation. In cancer especially in breast cancer annexin A1 influences tumor progression by interacting with proteins like COX-2 affecting tumor cell invasion and metastasis. The protein's role in these conditions highlights its potential as both a biomarker and therapeutic target helping in understanding disease mechanisms and developing treatment strategies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Annexin A1/ANXA1 antibody [EPR19342] (ab309365), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Annexin A1/ANXA1 antibody [EPR19342] (ab309365)

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labelling Annexin A1/ANXA1 with ab309365 at 1/100 (5.0 ug/ml). Positive staining on mouse spleen. The section was incubated with ab309365 for 40 mins at room temperature (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Annexin A1/ANXA1 antibody [EPR19342] (ab309365), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Annexin A1/ANXA1 antibody [EPR19342] (ab309365)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling Annexin A1/ANXA1 with ab309365 at 1/100 (5.0 ug/ml). Positive staining on human tonsils. The section was incubated with ab309365 for 40 mins at room temperature (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Annexin A1/ANXA1 antibody [EPR19342] (ab309365), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Annexin A1/ANXA1 antibody [EPR19342] (ab309365)

    Immunohistochemical analysis of paraffin-embedded Human breast tissue labelling Annexin A1/ANXA1 with ab309365 at 1/100 (5.0 ug/ml). Positive staining on human breast. The section was incubated with ab309365 for 40 mins at room temperature (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Annexin A1/ANXA1 antibody [EPR19342] (ab309365), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Annexin A1/ANXA1 antibody [EPR19342] (ab309365)

    Immunohistochemical analysis of paraffin-embedded Rat lung tissue labelling Annexin A1/ANXA1 with ab309365 at 1/100 (5.0 ug/ml). Positive staining on rat lung. The section was incubated with ab309365 for 40 mins at room temperature (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Annexin A1/ANXA1 antibody [EPR19342] (ab309365), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Annexin A1/ANXA1 antibody [EPR19342] (ab309365)

    Immunohistochemical analysis of paraffin-embedded Human bladder cancer tissue labelling Annexin A1/ANXA1 with ab309365 at 1/100 (5.0 ug/ml). Positive staining on human bladder cancer. The section was incubated with ab309365 for 40 mins at room temperature (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

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Product protocols

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