Rabbit Recombinant Monoclonal APC antibody - conjugated to Alexa Fluor® 647.
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Target Binding Affinity | Reactivity Expected | Dilution info - | Notes - |
Application Antibody Labelling | Reactivity Expected | Dilution info - | Notes - |
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Tumor suppressor. Promotes rapid degradation of CTNNB1 and participates in Wnt signaling as a negative regulator. APC activity is correlated with its phosphorylation state. Activates the GEF activity of SPATA13 and ARHGEF4. Plays a role in hepatocyte growth factor (HGF)-induced cell migration. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Associates with both microtubules and actin filaments, components of the cytoskeleton (PubMed:17293347). Plays a role in mediating the organization of F-actin into ordered bundles (PubMed:17293347). Functions downstream of Rho GTPases and DIAPH1 to selectively stabilize microtubules (By similarity). Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. It is required for the localization of MACF1 to the cell membrane and this localization of MACF1 is critical for its function in microtubule stabilization.
DP2.5, APC, Adenomatous polyposis coli protein, Protein APC, Deleted in polyposis 2.5
Rabbit Recombinant Monoclonal APC antibody - conjugated to Alexa Fluor® 647.
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
This antibody is predicted to detect isoform 2 (short) of APC based on sequence analysis.
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone. This conjugated antibody is eligible for the Abcam trial program.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
APC or Adenomatous Polyposis Coli is a multifunctional protein involved in several cellular processes. Its molecular weight is approximately 310 kDa. The APC protein is expressed in various tissues with higher concentrations found particularly in the colon and intestinal epithelial cells. Mechanically APC acts as a scaffold protein interacting with components of the cytoskeleton and regulating processes such as cell migration and adhesion. Its ability to bind to microtubules is key in its role in stabilizing them which affects cellular polarity and division.
APC plays a significant role in the regulation of the Wnt signaling pathway a critical mediator of cell growth and differentiation. APC forms part of a larger destruction complex that includes proteins like Axin and GSK-3β. This complex degrades β-catenin preventing its accumulation in the cell nucleus and therefore modulating gene transcription involved in cell proliferation. The ability of APC to mediate this process is important for maintaining proper cellular homeostasis and preventing uncontrolled cell growth that may lead to neoplasms.
APC is integral to the Wnt signaling and PI3K/Akt pathways. The Wnt pathway primarily concerns the regulation of cellular growth with APC facilitating the degradation of β-catenin therefore inhibiting the transcription of proliferative genes. Meanwhile in the PI3K/Akt pathway interactions between APC and β-catenin may influence cell survival and apoptosis. This connectivity to PI3K and Wnt pathways highlights APC's role in both promoting and inhibiting cell division processes depending on cellular context and signaling input.
APC is intrinsically linked to colorectal cancer and familial adenomatous polyposis (FAP). Mutations or loss of function in the APC gene lead to unregulated β-catenin levels promoting tumorigenesis in the colon. In colorectal cancer the absence or dysfunction of APC disrupts normal central pathways resulting in proliferative advantage to mutated cells. Connections to β-catenin further emphasize the mechanistic role APC plays in predisposing individuals to these diseases where abnormal Wnt pathway signaling becomes deleterious. Additionally disruption in APC function and the related pathways can alter interactions with other proteins like Axin and GSK-3β which are often involved in these disorders.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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