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AB225225

Alexa Fluor® 647 Anti-Aquaporin 1 antibody [EPR11588(B)]

5

(1 Review)

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(5 Publications)

Anti-Aquaporin 1 antibody [EPR11588(B)] - Alexa Fluor® 647 conjugated (ab225225) is a rabbit recombinant monoclonal antibody detecting Aquaporin 1 in ICC/IF. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

CHIP28, AQP1, Aquaporin-1, AQP-1, Aquaporin-CHIP, Channel-like integral membrane protein of 28 kDa, Urine water channel

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Aquaporin 1 antibody [EPR11588(B)] (AB225225)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Aquaporin 1 antibody [EPR11588(B)] (AB225225)

ab225225 staining Aquaporin 1 in 293T cells transfected with AQP1 with GFP tag. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab225225 at 1/100 dilution (pseudocolored in white) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue) and GFP is shown in green.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • 578 PE

    PE Anti-Aquaporin 1 antibody [EPR11588(B)]

  • 660 APC

    APC Anti-Aquaporin 1 antibody [EPR11588(B)]

  • HRP

    HRP Anti-Aquaporin 1 antibody [EPR11588(B)]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Aquaporin 1 antibody [EPR11588(B)]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Aquaporin 1 antibody [EPR11588(B)]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Aquaporin 1 antibody [EPR11588(B)]

  • Unconjugated

    Anti-Aquaporin 1 antibody [EPR11588(B)]

  • Carrier free

    Anti-Aquaporin 1 antibody [EPR11588(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11588(B)

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human, Human, Mouse, Rat

Applications

Flow Cyt (Intra), IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in 293T cells transfected with AQP1 with GFP tag fixed with 4% formaldehyde (10 min)</p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

What is this antibody validated in?
Alexa Fluor® 647 Anti-Aquaporin 1 antibody [EPR11588(B)] (ab225225) is a rabbit recombinant monoclonal antibody and is validated for use in Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

Related products
Antibody clone EPR11588(B) is also available pre-conjugated to a variety of labels for your convenience – Anti-Aquaporin 1 Alexa Fluor® 647 [EPR11588(B)] (ab225225).

Other related products
We have a range of other formats of antibody clone [EPR11588(B)] also available for your convenience: ab168387, Carrier free - ab178352, Alexa Fluor® 647 - ab225225, PE - ab303074, APC - ab303075, HRP - ab303076, Alkaline Phosphatase - ab308672, Alexa Fluor® 488 - ab309643, Alexa Fluor® 594 - ab310374, Alexa Fluor® 555 - ab314335

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aquaporin 1 also known as Aquaporin-1 AQP-1 or AQP1 is a protein that functions as a water channel facilitating the transport of water across cell membranes. This protein plays an important role in maintaining water homeostasis within cells. Aquaporin 1 has a mass of approximately 28 kDa and is encoded by the AQP1 gene. It is expressed in various tissues and organs including the kidneys lungs and red blood cells indicating its importance in diverse physiological processes.
Biological function summary

Aquaporin 1 is not part of a complex but independently contributes to maintaining osmotic balance and fluid movement across epithelial and endothelial tissues. The protein's selective permeability for water without allowing ions or other solutes highlights its specificity in controlling water transport which is essential for maintaining cellular environment stability.

Pathways

The aquaporin protein is involved in key biological pathways such as the osmoregulatory pathway and the fluid secretion pathway. It maintains efficient water transport in processes like urine concentration in the kidneys and fluid regulation in the pulmonary system. Aquaporins in general are related to similar transport proteins like sodium channels which together play an important role in maintaining osmotic balance across various tissues.

Aquaporin 1's dysfunction has been associated with conditions such as nephrogenic diabetes insipidus and pulmonary edema. These are linked to its role in water reabsorption in the kidneys and fluid balance in the lungs. The disorder's connection ties Aquaporin-1 with other regulatory proteins such as vasopressin receptors which work in conjunction to control renal water reabsorption and response to fluid imbalance.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Forms a water channel that facilitates the transport of water across cell membranes, playing a crucial role in water homeostasis in various tissues (PubMed : 1373524, PubMed : 23219802). Could also be permeable to small solutes including hydrogen peroxide, glycerol and gases such as amonnia (NH3), nitric oxide (NO) and carbon dioxide (CO2) (PubMed : 16682607, PubMed : 17012249, PubMed : 19273840, PubMed : 33028705, PubMed : 8584435). Recruited to the ankyrin-1 complex, a multiprotein complex of the erythrocyte membrane, it could be part of a CO2 metabolon, linking facilitated diffusion of CO2 across the membrane, anion exchange of Cl(-)/HCO3(-) and interconversion of dissolved CO2 and carbonic acid in the cytosol (PubMed : 17012249, PubMed : 35835865). In vitro, it shows non-selective gated cation channel activity and may be permeable to cations like K(+) and Na(+) in vivo (PubMed : 36949749, PubMed : 8703053).
See full target information AQP1
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40565279

2025

Molecular Determinants of the Human Retinal Pigment Epithelium Cell Fate and Potential Pharmacogenomic Targets for Precision Medicine.

Applications

Unspecified application

Species

Unspecified reactive species

Cristina Zibetti

Nanoscale advances 6:276-286 PubMed38125591

2023

Lithographically defined encoded magnetic heterostructures for the targeted screening of kidney cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Selma Leulmi Pichot,Tarun Vemulkar,Jeroen Verheyen,Lauren Wallis,James O Jones,Andrew P Stewart,Sarah J Welsh,Grant D Stewart,Russell P Cowburn

Nature immunology 23:1256-1272 PubMed35902638

2022

Partial RAG deficiency in humans induces dysregulated peripheral lymphocyte development and humoral tolerance defect with accumulation of T-bet B cells.

Applications

Unspecified application

Species

Unspecified reactive species

Krisztian Csomos,Boglarka Ujhazi,Peter Blazso,Jose L Herrera,Christopher M Tipton,Tomoki Kawai,Sumai Gordon,Maryssa Ellison,Kevin Wu,Matthew Stowell,Lauren Haynes,Rachel Cruz,Bence Zakota,Johnny Nguyen,Michelle Altrich,Christoph B Geier,Svetlana Sharapova,Joseph F Dasso,Jennifer W Leiding,Grace Smith,Waleed Al-Herz,Mayra de Barros Dorna,Olajumoke Fadugba,Eva Fronkova,Veronika Kanderova,Michael Svaton,Sarah E Henrickson,Joseph D Hernandez,Taco Kuijpers,Snezhina Mihailova Kandilarova,Elizaveta Naumova,Tomas Milota,Anna Sediva,Despina Moshous,Benedicte Neven,Tara Saco,Ravishankar Sargur,Sinisa Savic,John Sleasman,Gauri Sunkersett,Brant R Ward,Masanobu Komatsu,Stefania Pittaluga,Attila Kumanovics,Manish J Butte,Michael P Cancro,Shiv Pillai,Eric Meffre,Luigi D Notarangelo,Jolan E Walter

JCI insight 7: PubMed34941574

2022

DDR1 contributes to kidney inflammation and fibrosis by promoting the phosphorylation of BCR and STAT3.

Applications

Unspecified application

Species

Unspecified reactive species

Corina M Borza,Gema Bolas,Fabian Bock,Xiuqi Zhang,Favour C Akabogu,Ming-Zhi Zhang,Mark de Caestecker,Min Yang,Haichun Yang,Ethan Lee,Leslie Gewin,Agnes B Fogo,W Hayes McDonald,Roy Zent,Ambra Pozzi

iScience 24:103308 PubMed34820603

2021

Kidney disease risk factors do not explain impacts of low dietary protein on kidney function and structure.

Applications

Unspecified application

Species

Unspecified reactive species

Amelia K Fotheringham,Samantha M Solon-Biet,Helle Bielefeldt-Ohmann,Domenica A McCarthy,Aisling C McMahon,Kari Ruohonen,Isaac Li,Mitchell A Sullivan,Rani O Whiddett,Danielle J Borg,Victoria C Cogger,William O Ballard,Nigel Turner,Richard G Melvin,David Raubenheimer,David G Le Couteur,Stephen J Simpson,Josephine M Forbes
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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