AB215225

Alexa Fluor® 647 Anti-Aquaporin 5 antibody [EPR3747]

RabMAb
Recombinant
What is this?

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(7 Publications)

Rabbit Recombinant Monoclonal Aquaporin 5 antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-P and reacts with Human samples. Cited in 7 publications.

View Alternative Names

Aquaporin-5, AQP-5, AQP5

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Aquaporin 5 antibody [EPR3747] (AB215225)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Aquaporin 5 antibody [EPR3747] (AB215225)

IHC image of Aquaporin 5 staining in a section of formalin-fixed paraffin-embedded normal human colon*.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) in a Dako Pascal pressure cooker using the standard factory-set regime. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab215225 at 1/1000 dilution (shown in red) and counterstained using ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount^®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Aquaporin 5 antibody [EPR3747]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Aquaporin 5 antibody [EPR3747]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3747

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol." } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein known as Aquaporin 5 often abbreviated as AQP5 is a member of the aquaporin family which are integral membrane proteins that function as channels for water. This particular protein facilitates water transport across cell membranes an action critical in maintaining fluid balance. Aquaporin 5 has a molecular mass of approximately 29 kDa. It is largely expressed in epithelial cells in various tissues including the salivary glands lacrimal glands and respiratory tissues. It is recognized by different names such as Aquaporin-5 and sometimes referenced in studies as Aquazol 5.

Biological function summary

Water transport and secretion processes heavily rely on the presence of Aquaporin 5. It is integral in the formation of tight junctions in epithelial cells enhancing cell permeability to water. Unlike some other aquaporins Aquaporin 5 operates independently and is not known to be part of a larger protein complex. Its presence influences the efficiency of fluid secretion affecting activities such as salivation and tear secretion. The capacity of AQP5 to control water movement tightly connects it to cellular hydration homeostasis.

Pathways

Aquaporin 5 is significantly involved in the regulation of water balance and osmotic equilibrium in epithelial tissues. Its role in fluid secretion pathways is interconnected with other proteins like Aquaporin 4 which share similar duties in the osmotic control of water. In the water transport pathways AQP5 coordinates with proteins like CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) to manage fluid movement particularly in the respiratory passages and glands. The precise regulation of water flow by these pathways ensures that overall tissue hydration remains constant.

Aquaporin 5 is directly associated with conditions such as Sjögren's syndrome and xerostomia (dry mouth syndrome). During Sjögren's syndrome malfunction of AQP5 can lead to impaired salivary and tear secretion contributing to the symptoms of dryness. Furthermore defects or polymorphisms in AQP5 have implications in respiratory diseases potentially affecting proteins like Aquaporin 3 which shares functional similarities. Understanding the role of AQP5 in these conditions enables further exploration into therapeutic targets and treatment methods.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Aquaporins form homotetrameric transmembrane channels, with each monomer independently mediating water transport across the plasma membrane along its osmotic gradient (PubMed : 18768791, PubMed : 8621489). Plays an important role in fluid secretion in salivary glands (By similarity). Required for TRPV4 activation by hypotonicity. Together with TRPV4, controls regulatory volume decrease in salivary epithelial cells (PubMed : 16571723). Seems to play a redundant role in water transport in the eye, lung and in sweat glands (By similarity).

See full target information AQP5

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

International urogynecology journal 36:1523-1531 PubMed40478270

2025

Expressions of IGF-1, pAKT, and pS6 in Uterosacral Ligaments of Patients with Pelvic Organ Prolapse.

Applications

Unspecified application

Species

Unspecified reactive species

Hongling Xu,Gensheng Wang,Qing Li,Xuemei Wang,Dongdi Xu

JCI insight 10: PubMed40178918

2025

Dipeptidase-1-knockout mice develop invasive tumors with features of microsatellite-unstable colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah E Glass,Matthew E Bechard,Zheng Cao,Radhika Aramandla,Ping Zhao,Samuel T Ellis,Emily H Green,Elizabeth G Fisher,Ryan T Smith,Chelsie K Sievers,Maria Johnson Irudayam,Frank Revetta,M Kay Washington,Gregory D Ayers,Cody N Heiser,Alan J Simmons,Yanwen Xu,Yu Wang,Annika Windon,Martha J Shrubsole,Nicholas O Markham,Qi Liu,Ken S Lau,Robert J Coffey

iScience 26:106773 PubMed37216094

2023

Major depression-related factor NEGR1 controls salivary secretion in mouse submandibular glands.

Applications

Unspecified application

Species

Unspecified reactive species

Jisoo Lee,Soohyun Kim,Boram Lee,Yoo-Bin Kim,Kwang Hwan Kim,Gehoon Chung,Sung Joong Lee,Soojin Lee,Woong Sun,Hee-Kyung Park,Se-Young Choi

Military Medical Research 9:13 PubMed35351192

2022

Small molecules facilitate single factor-mediated sweat gland cell reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai-Fei Ji,Lai-Xian Zhou,Zhi-Feng Sun,Jiang-Bing Xiang,Shao-Yuan Cui,Yan Li,Hua-Ting Chen,Yi-Qiong Liu,Huan-Huan Gao,Xiao-Bing Fu,Xiao-Yan Sun

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 8:e2103079 PubMed34569165

2021

Sweat Gland Organoids Originating from Reprogrammed Epidermal Keratinocytes Functionally Recapitulated Damaged Skin.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyan Sun,Jiangbing Xiang,Runkai Chen,Zhijun Geng,Lintao Wang,Yiqiong Liu,Shuaifei Ji,Huating Chen,Yan Li,Cuiping Zhang,Peng Liu,Tao Yue,Lei Dong,Xiaobing Fu

International journal of molecular sciences 22: PubMed33946369

2021

Muscarinic Receptors and BK Channels Are Affected by Lipid Raft Disruption of Salivary Gland Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jisoo Lee,Yoon-Jung Kim,La-Mee Choi,Keimin Lee,Hee-Kyung Park,Se-Young Choi

Nature 578:437-443 PubMed32025032

2020

AQP5 enriches for stem cells and cancer origins in the distal stomach.

Applications

Unspecified application

Species

Unspecified reactive species

Si Hui Tan,Yada Swathi,Shawna Tan,Jasmine Goh,Ryo Seishima,Kazuhiro Murakami,Masanobu Oshima,Toshikatsu Tsuji,Phyllis Phuah,Liang Thing Tan,Esther Wong,Aliya Fatehullah,Taotao Sheng,Shamaine Wei Ting Ho,Heike I Grabsch,Supriya Srivastava,Ming Teh,Simon L I J Denil,Seri Mustafah,Patrick Tan,Asim Shabbir,Jimmy So,Khay Guan Yeoh,Nick Barker

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 647 Anti-Aquaporin 5 antibody [EPR3747]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Aquaporin 5 antibody [EPR3747] (AB215225)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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