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AB207645

Alexa Fluor® 647 Anti-ARF1 + ARF3 antibody [EPR443]

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(1 Publication)

Rabbit Recombinant Monoclonal ARF1 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

ADP-ribosylation factor 1, ARF1

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ARF1 + ARF3 antibody [EPR443] (AB207645)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ARF1 + ARF3 antibody [EPR443] (AB207645)

ab207645 staining ARF1 + ARF3 in CaCo2 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab207645 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR443

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in CaCo2 cells fixed with 100% methanol (5 min)</p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ARF1 and ARF3 also known as ADP-ribosylation factors 1 and 3 are small GTP-binding proteins belonging to the ARF family. ARF1 has a molecular mass of approximately 21 kDa. These proteins are expressed in most eukaryotic cells where they play roles in vesicular trafficking processes. ARF1 is mainly located in the Golgi apparatus while ARF3 appears in the plasma membrane and cytoplasm. They function as molecular switches by cycling between active GTP-bound and inactive GDP-bound states which regulates the formation and budding of vesicles.
Biological function summary

ARF1 and ARF3 are significant in the intracellular trafficking of proteins and lipids. The proteins are part of the coatomer protein complex (COPI) important for retrograde transport from the Golgi to the endoplasmic reticulum. By recruiting coat proteins to the Golgi membranes ARF1 and ARF3 facilitate the sorting and packaging of cargo molecules into transport vesicles. This process sustains the maintenance of organelle identity and the dynamics of membrane remodeling.

Pathways

ARF1 and ARF3 are involved in key cellular pathways particularly those related to vesicular trafficking and membrane dynamics. They participate in the COPI and clathrin-mediated pathways. Through these pathways ARF1 interacts with proteins like PDZD8 while ARF3 works with proteins involved in primary cilia formation. These interactions underline their integrative role in maintaining proper cellular organization and communication.

ARF1 and ARF3 have links to cancer and neurodegenerative diseases. Dysregulation of ARF1 is associated with various cancer types due to its role in cell proliferation and migration. ARF3 has connections to neurodegenerative disorders where altered vesicular trafficking can lead to pathological conditions. ARF1's interaction with the protein NFDM-442 highlights its importance in disease contexts whereby changes in these interactions might contribute to disease progression and severity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Small GTPase involved in protein trafficking between different compartments (PubMed : 8253837). Modulates vesicle budding and uncoating within the Golgi complex (PubMed : 8253837). In its GTP-bound form, triggers the recruitment of coatomer proteins to the Golgi membrane (PubMed : 8253837). The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles (PubMed : 8253837). The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity; the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasticity of excitatory synapses and spine shrinkage during long-term depression (LTD) (By similarity). Plays a key role in the regulation of intestinal stem cells and gut microbiota, and is essential for maintaining intestinal homeostasis (By similarity). Plays also a critical role in mast cell expansion but not in mast cell maturation by facilitating optimal mTORC1 activation (By similarity).. (Microbial infection) Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase.
See full target information ARF1

Additional targets

ADP-ribosylation factor 3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

BMC geriatrics 25:645 PubMed40836274

2025

AIMP1 exerts hearing protection role in age related hearing loss mice by regulating SIRT1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Haisen Peng,Jiali Liu,Yuehui Liu,Chunhua Li,Zhilin Zhang,Shuihua Hu,Wen Xie
View all publications
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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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