Rabbit Recombinant Monoclonal ARPC5/p16 ARC antibody - conjugated to Alexa Fluor® 647.
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Antibody Labelling | Reactivity Expected | Dilution info - | Notes - |
Application Target Binding Affinity | Reactivity Expected | Dilution info - | Notes - |
Component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed:9230079). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed:9230079). In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed:29925947). The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed:29925947).
ARC16, ARPC5, Actin-related protein 2/3 complex subunit 5, Arp2/3 complex 16 kDa subunit, p16-ARC
Rabbit Recombinant Monoclonal ARPC5/p16 ARC antibody - conjugated to Alexa Fluor® 647.
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
This antibody is specific to the p16 ARC protein O15511. There is no observed cross reactivity to the CDKN2A/p16INK4a protein.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone. This conjugated antibody is eligible for the Abcam trial program.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
ARPC5 also known as p16 ARC is a protein with a molecular weight of roughly 16 kilodaltons. It localizes largely in the cytoplasm and expresses in many cell types across species. The ARPC5 protein is a subunit of the Arp2/3 complex a vital component responsible for actin nucleation and branching during cytoskeleton remodeling. Researchers often examine ARPC5 through techniques such as western blot and immunohistochemistry using specific antibodies like anti-p16 or ARPC5 anticuerpo to identify its presence and distribution.
The ARPC5 protein is essential for effective actin polymerization making it an important contributor to cell motility and structure. As part of the Arp2/3 complex it interacts with other proteins to stimulate the growth of new actin filaments. This activity is important in processes like endocytosis phagocytosis and cell migration. Furthermore ARPC5 plays a role in maintaining cellular homeostasis and is involved in cellular signaling pathways.
Arpc5 influences the regulation of the actin cytoskeleton which intersects with pathways associated with cell movement and morphogenesis. It prominently relates to the WAVE complex working synergistically to regulate actin dynamics. ARPC5's involvement in the Rac1-WAVE-Arp2/3 pathway highlights its relationship with Rac1 a small GTPase known for regulating the cytoskeleton and cellular functions like adhesion and migration.
Imbalances in ARPC5 function can link to cancer progression and neurodegenerative diseases. Aberrant activity of the Arp2/3 complex which involves ARPC5 associates with increased cell invasion and metastasis in cancer. Additionally disruptions in ARPC5 activity connect to neurodegenerative conditions where cytoskeletal arrangement alterations play a role. The protein's interaction with others in the complex such as Rac1 further deepens its involvement in these diseases signaling potential targets for therapeutic intervention.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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