AB196198

Alexa Fluor® 647 Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker

RabMAb
Recombinant
What is this?

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(3 Publications)

Anti-ATP5A antibody [EPR13030(B)] - Alexa Fluor® 647 conjugated (ab196198) is a rabbit recombinant monoclonal antibody detecting ATP5A in Flow Cytometry (Intra), ICC/IF. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

ATP5A, ATP5A1, ATP5AL2, ATPM, ATP5F1A, ATP synthase F1 subunit alpha

2 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (AB196198)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (AB196198)

ab196198 staining ATP5A in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab196198 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor^® 488, shown in green) at 2μg/ml overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (AB196198)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (AB196198)

Overlay histogram showing HeLa cells stained with ab196198 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab196198, 1/50 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 647 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a solid-state 25mW red diode laser (635 nm) and 675/30 bandpass filter.

This antibody gave a positive signal in HeLa fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

HRP

HRP Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker
Unconjugated

Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker
Carrier free

Anti-ATP5A antibody [EPR13030(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13030(B)

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

Reacts with

Human, Human, Mouse, Rat

Applications

Flow Cyt (Intra), IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/alexa-fluor-647-rabbit-igg-monoclonal-epr25a-isotype-control-ab199093'>ab199093</a> - Rabbit monoclonal IgG (Alexa Fluor®; 647), is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

What is this antibody validated in?
Alexa Fluor® 647 Anti-ATP5A antibody [EPR13030(B)] (ab196198) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

Related products
Antibody clone EPR13030(B) is also available pre-conjugated to a variety of labels for your convenience – Anti-ATP5A Alexa Fluor® 647 [EPR13030(B)] (ab196198).

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATP5A also known as ATP synthase F1 subunit alpha is a protein important for cellular energy production. As part of the ATP synthase complex it plays a mechanical role in synthesizing ATP from ADP and inorganic phosphate. The ATP5A protein has a molecular weight of approximately 55 kDa and is widely expressed in the inner mitochondrial membrane across different cell types. Its central function lies in its ability to harness the energy of the proton gradient generated by the electron transport chain to catalyze ATP synthesis.

Biological function summary

ATP5A is essential in cellular respiration serving as a catalytic core of the F1 component of ATP synthase. As part of the multi-subunit enzyme complex ATP synthase is responsible for ATP production the primary energy currency in cells. The ATP5A subunit works in conjunction with other subunits of the enzyme oligomer to facilitate the conversion of energy released during oxidative phosphorylation into a usable form. The protein's efficiency in this biological role underpins its importance in sustaining cellular energy homeostasis.

Pathways

ATP5A plays a pivotal role in oxidative phosphorylation and the electron transport chain integral components of cellular respiration. The oxidative phosphorylation pathway depends on this protein to manage the synthesis of ATP molecules while the electron transport chain creates the proton gradient necessary for ATP production. ATP5A is functionally connected to other proteins in these pathways such as ATP5B and cytochrome c oxidase working in a coordinated manner to ensure efficient energy transfer and maintenance.

ATP5A is implicative in mitochondrial disorders and neurodegenerative diseases such as Leigh syndrome and Parkinson's disease. These conditions often arise from deficits in ATP production where ineffective ATP synthase activity can contribute to cellular energy failures. In the context of Parkinson’s disease for instance ATP5A interactions with other proteins like Parkin can contribute to mitochondrial dysfunction an important pathological feature of the disorder. Through such associations alterations in ATP5A activity can significantly impact disease progression and symptomatology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Subunit alpha, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain (Probable). ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel (PubMed : 37244256). These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk (PubMed : 37244256). During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation (Probable). In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro (By similarity). With the catalytic subunit beta (ATP5F1B), forms the catalytic core in the F(1) domain (PubMed : 37244256). Subunit alpha does not bear the catalytic high-affinity ATP-binding sites (Probable). Binds the bacterial siderophore enterobactin and can promote mitochondrial accumulation of enterobactin-derived iron ions (PubMed : 30146159).

See full target information ATP5F1A

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:6152 PubMed39034312

2024

Glutathione synthesis in the mouse liver supports lipid abundance through NRF2 repression.

Applications

Unspecified application

Species

Unspecified reactive species

Gloria Asantewaa,Emily T Tuttle,Nathan P Ward,Yun Pyo Kang,Yumi Kim,Madeline E Kavanagh,Nomeda Girnius,Ying Chen,Katherine Rodriguez,Fabio Hecht,Marco Zocchi,Leonid Smorodintsev-Schiller,TashJaé Q Scales,Kira Taylor,Fatemeh Alimohammadi,Renae P Duncan,Zachary R Sechrist,Diana Agostini-Vulaj,Xenia L Schafer,Hayley Chang,Zachary R Smith,Thomas N O'Connor,Sarah Whelan,Laura M Selfors,Jett Crowdis,G Kenneth Gray,Roderick T Bronson,Dirk Brenner,Alessandro Rufini,Robert T Dirksen,Aram F Hezel,Aaron R Huber,Joshua Munger,Benjamin F Cravatt,Vasilis Vasiliou,Calvin L Cole,Gina M DeNicola,Isaac S Harris

Nature communications 15:2203 PubMed38467616

2024

Mitochondrial metabolism sustains CD8 T cell migration for an efficient infiltration into solid tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Luca Simula,Mattia Fumagalli,Lene Vimeux,Irena Rajnpreht,Philippe Icard,Gary Birsen,Dongjie An,Frédéric Pendino,Adrien Rouault,Nadège Bercovici,Diane Damotte,Audrey Lupo-Mansuet,Marco Alifano,Marie-Clotilde Alves-Guerra,Emmanuel Donnadieu

The Journal of experimental medicine 220: PubMed36995340

2023

Phagocytosis increases an oxidative metabolic and immune suppressive signature in tumor macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Michael A Gonzalez,Daniel R Lu,Maryam Yousefi,Ashley Kroll,Chen Hao Lo,Carlos G Briseño,J E Vivienne Watson,Sergey Novitskiy,Vanessa Arias,Hong Zhou,Andres Plata Stapper,Min K Tsai,Emily L Ashkin,Christopher W Murray,Chi-Ming Li,Monte M Winslow,Kristin V Tarbell

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 647 Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (AB196198)

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (AB196198)

Related conjugates and formulations (5)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise