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AB317528

Alexa Fluor® 647 Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401]

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Rabbit Recombinant Monoclonal ATP6V1B1 antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-P and reacts with Human, Mouse, Rat samples.

View Alternative Names

ATP6B1, VATB, VPP3, ATP6V1B1, V-ATPase subunit B 1, Endomembrane proton pump 58 kDa subunit, Vacuolar proton pump subunit B 1

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401] (AB317528)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401] (AB317528)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscl tissue labeling ATP6V1B1 + ATP6V1B2 with ab317528 at 1/50 (10.0 ug/ml) dilution.

Negative control : no staining on human skeletal muscle.The section was incubated with ab317528 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND RX instrument.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401] (AB317528)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401] (AB317528)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ATP6V1B1 + ATP6V1B2 with ab317528 at 1/50 (10.0 ug/ml) dilution.

Cytoplasmic staining on human kidney.The section was incubated with ab317528 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401] (AB317528)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401] (AB317528)

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling ATP6V1B1 + ATP6V1B2 with ab317528 at 1/50 (10.0 ug/ml) dilution.

Cytoplasmic staining on rat kidney.The section was incubated with ab317528 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND RX instrument.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401] (AB317528)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401] (AB317528)

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling ATP6V1B1 + ATP6V1B2 with ab317528 at 1/50 (10.0 ug/ml) dilution.

Cytoplasmic staining on mouse kidney.The section was incubated with ab317528 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND RX instrument.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

  • Unconjugated

    Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401]

  • Carrier free

    Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16401

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATP6V1B1 and ATP6V1B2 are subunits of the V-type proton ATPase (vacuolar ATPase V-ATPase) important for proton translocation across intracellular membranes. Known also as ATP synthase subunits B1 and B2 these proteins together with other subunits form part of the cytoplasmic V1 domain of the enzyme. ATP6V1B1 has a molecular mass of approximately 56 kDa while ATP6V1B2 is slightly larger at around 57 kDa. Both subunits are widely expressed in various tissues but are specifically abundant in the kidney and inner ear.
Biological function summary

ATP6V1B1 and ATP6V1B2 participate in acidification of intracellular environments a fundamental process for cellular homeostasis and function. They are integral components of the V-ATPase complex which actively transports protons into lysosomes endosomes and other vesicles generating acidic conditions essential for protein degradation vesicular trafficking and hormone activation. This acidification is also important for maintaining the optimal pH necessary for various enzymatic activities within organelles.

Pathways

These subunits play an important role in the regulation of the mTOR signaling pathway and endocytosis pathway both critical for cell growth proliferation and nutrient sensing. The activity of V-ATPase influences these pathways by affecting pH-dependent processes required for receptor-mediated endocytosis and nutrient availability. ATP6V1B1 and ATP6V1B2 interact with other V-ATPase subunits such as ATP6V1A and ATP6V1C to modulate their function in these pathways.

Mutations and dysfunctions in ATP6V1B1 and ATP6V1B2 are linked to distal renal tubular acidosis (dRTA) and sensorineural hearing loss. These conditions result from altered proton transport and subsequent disruptions in acid-base balance. The relationship with dRTA involves the defective acidification of urine a process dependent on V-ATPase activity. Additionally altered function of these subunits has implications for their interaction with other proton pumps and transport proteins such as the carbonic anhydrase II which work together in maintaining pH equilibrium in the kidney and inner ear.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Non-catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons (PubMed : 16769747). V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment (PubMed : 32001091). Essential for the proper assembly and activity of V-ATPase (PubMed : 16769747). In renal intercalated cells, mediates secretion of protons (H+) into the urine thereby ensuring correct urinary acidification (PubMed : 16769747). Required for optimal olfactory function by mediating the acidification of the nasal olfactory epithelium (By similarity).
See full target information ATP6V1B1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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