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Mouse Monoclonal ATPase Inhibitory Factor 1/IF1 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human samples.

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Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ATPase Inhibitory Factor 1/IF1 antibody [5E2D7] (AB198077), expandable thumbnail

Key facts

Isotype

IgG1

Host species

Mouse

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IF
Human
Tested
Mouse
Predicted
Rat
Predicted
Cow
Predicted

Tested
Tested

Species

Human

Dilution info

1/100

Notes

This product gave a positive signal in HepG2 cells fixed with 4% formaldehyde (10 min).

Predicted
Predicted

Species

Mouse, Rat, Cow

Dilution info

-

Notes

-

Associated Products

Select an associated product type

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1 product for Alternative Version

Target data

Function

Endogenous F(1)F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F(1)F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix. Required to avoid the consumption of cellular ATP when the F(1)F(o)-ATP synthase enzyme acts as an ATP hydrolase (By similarity). Indirectly acts as a regulator of heme synthesis in erythroid tissues: regulates heme synthesis by modulating the mitochondrial pH and redox potential, allowing FECH to efficiently catalyze the incorporation of iron into protoporphyrin IX to produce heme (By similarity).

Alternative names

Recommended products

Mouse Monoclonal ATPase Inhibitory Factor 1/IF1 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human samples.

Key facts

Isotype

IgG1

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

5E2D7

Purification technique

Affinity purification

Light chain type

kappa

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

ATPase Inhibitory Factor 1 (IF1) also referred to as IF1 protein ATPIF1 or ATPase inhibitory factor 1 is a small protein with a mass of approximately 12 kDa. It is expressed primarily in mitochondria-rich tissues such as the heart liver and brain where it plays an important role in energy metabolism. IF1 is notable for its ability to bind to the F1 subunit of the mitochondrial ATP synthase inhibiting its ATPase activity under specific conditions.

Biological function summary

IF1 influences mitochondrial function by regulating ATP synthase in response to changes in the cellular environment. Under conditions of low cellular energy IF1 stabilizes and prevents hydrolysis of ATP by binding to the F1 catalytic domain of the enzyme. This regulation helps to conserve ATP critical for maintaining cellular viability during stress. IF1 can also interact with other mitochondrial proteins to form complexes that influence energy homeostasis in the cell.

Pathways

IF1 integrates into pathways involved in cellular energy management including oxidative phosphorylation and ATP synthesis pathways. It interacts significantly with proteins such as the mitochondrial F1Fo-ATP synthase complex. By modulating ATP synthase activity IF1 ensures efficient ATP production and maintains mitochondrial membrane potential important for proper cell function.

Associated diseases and disorders

IF1 has associations with metabolic conditions and cancer. Abnormal expression or activity of IF1 correlates with mitochondrial dysfunction often observed in cancer cells where it affects cell proliferation. Additionally IF1 has links to cardiac conditions given its role in regulating heart metabolism. It relates to proteins like the cytochrome c oxidase influencing cellular respiration and affecting disease progression.

Product promise

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1 product image

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ATPase Inhibitory Factor 1/IF1 antibody [5E2D7] (ab198077), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ATPase Inhibitory Factor 1/IF1 antibody [5E2D7] (ab198077)

    ab198077 staining ATPase Inhibitory Factor 1/IF1 in HepG2 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198077 at a 1/100 dilution (shown in red) and Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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Product protocols

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