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AB300094

Alexa Fluor® 647 Anti-BCMA antibody [EPR22457-260]

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Anti-BCMA antibody [EPR22457-260] - Alexa Fluor® 647 conjugated (ab300094) is a rabbit recombinant monoclonal antibody detecting BCMA in Flow Cytometry, ICC/IF. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

CD269, BCM, BCMA, TNFRSF17, Tumor necrosis factor receptor superfamily member 17, B-cell maturation protein

2 Images
Flow Cytometry - Alexa Fluor® 647 Anti-BCMA antibody [EPR22457-260] (AB300094)
  • Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 647 Anti-BCMA antibody [EPR22457-260] (AB300094)

Flow Cytometry analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) (Left) / U266B1 (human multiple myeloma B lymphocyte cell line) (Right), labelling BCMA with ab300094 at a 1/500 dilution. Isotype control - Rabbit monoclonal isotype control - Alexa Fluor® 647 (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue) Gated on viable cells. Negative control : HEK-293T (PMID : 18025285).

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-BCMA antibody [EPR22457-260] (AB300094)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-BCMA antibody [EPR22457-260] (AB300094)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U266B1 (human multiple myeloma B lymphocytes) labelling BCMA with ab300094 at 1/50 dilution (red). Confocal image showing cytoplasmic and weak membranous staining in U266B1 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) (green) at 1/200 dilution.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22457-260

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human

Applications

ICC/IF, Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p></p>" } } }

Product details

What is this antibody validated in?
Alexa Fluor® 647 Anti-BCMA antibody [EPR22457-260] (ab300094) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Related products
Antibody clone EPR22457-260 is also available pre-conjugated to a variety of labels for your convenience – Anti-BCMA Alexa Fluor® 647 [EPR22457-260] (ab300094).

Other related products
We have a range of other formats of antibody clone [EPR22457-260] also available for your convenience: ab253242, Carrier free - ab254205, Alexa Fluor® 488 - ab284647, Alexa Fluor® 647 - ab300094

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

The protein expressed by the TNFRSF17 gene acts as a receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. It promotes B-cell survival and plays a role in regulating humoral immunity, while also activating NF-kappa-B and JNK. This supplementary information is collated from multiple sources and compiled automatically.
See full target information TNFRSF17

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com