AB187602

Alexa Fluor® 647 Anti-beta 2 Microglobulin antibody [B2M01]

Name: Alexa Fluor® 647 Anti-beta 2 Microglobulin antibody [B2M01] (ab187602) | Abcam
Brand: Abcam Limited
SKU: AB187602
Price: 535 USD
Availability: InStock

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(2 Publications)

Mouse Monoclonal beta 2 Microglobulin antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF, Flow Cyt and reacts with Transfected cell line - Human, Human samples. Cited in 2 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human B2M.

View Alternative Names

CDABP0092, HDCMA22P, B2M, Beta-2-microglobulin

2 Images

Flow Cytometry - Alexa Fluor® 647 Anti-beta 2 Microglobulin antibody [B2M01] (AB187602)

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 647 Anti-beta 2 Microglobulin antibody [B2M01] (AB187602)

Flow Cytometry analysis of lymphocytes stained using ab187602 (red) at 5μg/ml, separated from unstained lymphocytes (black-dashed).

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-beta 2 Microglobulin antibody [B2M01] (AB187602)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-beta 2 Microglobulin antibody [B2M01] (AB187602)

Immunocytochemistry/ Immunofluorescence of Human beta 2 Microglobulin-transfected canine cells labeled A) with ab187602 and B) with Alexa Fluor® 647 conjugated Mouse IgG2a Isotype Control. Cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Tween20 containing 2% BSA. Antibody concentration 2μg/ml.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

B2M01

Isotype

IgG2a

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

Reacts with

Human

Applications

Flow Cyt, ICC/IF

applications

Immunogen

Native Full Length Protein corresponding to Human B2M.

P61769

Specificity

ab187602 reacts with beta 2 microglobulin associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta 2 microglobulin.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-5 µg/mL", "FlowCyt-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Chicken": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Cow": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Dog": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Rabbit": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Transfected cell line - Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta-2-Microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC I) and plays an important role in presenting peptides to the immune system. B2M weighs approximately 11.8 kDa and is found abundantly in all nucleated cells. It has alternate names such as B2 microglobulin or beta-2-microglobulin. This protein is present in the cell membrane as a part of the MHC I which is important for immune surveillance. Additionally B2M is detectable in various biological fluids including serum and its levels can reflect physiological and pathological states.

Biological function summary

Beta-2-microglobulin is important for the stability and transport of MHC class I molecules to the cell surface. As part of the MHC class I complex B2M assists in binding peptides allowing immune cells to identify and target pathogen-infected cells. Without B2M the MHC class I molecules are not properly expressed on the cell surface disrupting immune recognition. In laboratory settings researchers often use anti-beta-2-microglobulin antibodies to investigate its role in MHC class I function.

Pathways

Beta-2-microglobulin interacts significantly with the immune system most notably in the antigen processing and presentation pathway. It works alongside proteins such as the heavy chain of MHC class I. B2M is important in the pathway that involves the transport of antigens to the endoplasmic reticulum where they are loaded onto MHC class I molecules for inspection by cytotoxic T cells. Another related pathway is the tapasin-mediated processing of antigen peptides highlighting the indispensable role of B2M in immune response regulation.

Beta-2-microglobulin is associated with conditions such as beta-2-microglobulin amyloidosis and certain lymphoproliferative disorders. Elevated levels of B2M in serum serve as a marker for diseases like multiple myeloma where the protein level correlates with disease severity. B2M-related amyloidosis frequently occurs in patients undergoing long-term dialysis where amyloid deposits accumulate in tissues. Linking B2M to immune system dysfunction studies have shown interactions with other proteins including components of the immune system like HLA-A and HLA-B highlighting B2M's relevance in diagnosing and understanding these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Exogenously applied M.tuberculosis EsxA or EsxA-EsxB (or EsxA expressed in host) binds B2M and decreases its export to the cell surface (total protein levels do not change), probably leading to defects in class I antigen presentation (PubMed : 25356553).

See full target information B2M

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

ACS synthetic biology 11:2405-2416 PubMed35687717

2022

Proteome-Scale Screening to Identify High-Expression Signal Peptides with Minimal N-Terminus Biases via Yeast Display.

Applications

Unspecified application

Species

Unspecified reactive species

Patrick V Holec,Karen V Camacho,Kathryn C Breuckman,Jody Mou,Michael E Birnbaum

Cell proliferation 50: PubMed27910163

2016

Serum amyloid A, an acute phase protein, stimulates proliferative and proinflammatory responses of keratinocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Ning Yu,Shujie Zhang,Jiajing Lu,Ying Li,Xuemei Yi,Li Tang,Lina Su,Yangfeng Ding

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 647 Anti-beta 2 Microglobulin antibody [B2M01]

Flow Cytometry - Alexa Fluor® 647 Anti-beta 2 Microglobulin antibody [B2M01] (AB187602)

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-beta 2 Microglobulin antibody [B2M01] (AB187602)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise