Knockout Tested Rabbit Recombinant Monoclonal beta Catenin antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-beta Catenin non-phospho(active) S45 antibody [EPR26155-110-1] (AB316940), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-647-beta-catenin-non-phosphoactive-s45-antibody-epr26155-110-1-ab316940--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img428536.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-beta Catenin non-phospho(active) S45 antibody [EPR26155-110-1] (AB316940)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-beta Catenin non-phospho(active) S45 antibody [EPR26155-110-1] (AB316940), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-647-beta-catenin-non-phosphoactive-s45-antibody-epr26155-110-1-ab316940--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img428537.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-beta Catenin non-phospho(active) S45 antibody [EPR26155-110-1] (AB316940)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-beta Catenin non-phospho(active) S45 antibody [EPR26155-110-1] (AB316940), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-647-beta-catenin-non-phosphoactive-s45-antibody-epr26155-110-1-ab316940--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img428538.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-beta Catenin non-phospho(active) S45 antibody [EPR26155-110-1] (AB316940)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-beta Catenin non-phospho(active) S45 antibody [EPR26155-110-1] (AB316940), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-647-beta-catenin-non-phosphoactive-s45-antibody-epr26155-110-1-ab316940--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img428724.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-beta Catenin non-phospho(active) S45 antibody [EPR26155-110-1] (AB316940)")
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-beta Catenin non-phospho(active) S45 antibody [EPR26155-110-1] (AB316940), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-647-beta-catenin-non-phosphoactive-s45-antibody-epr26155-110-1-ab316940--immunocytochemistry-immunofluorescence-img428535.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-beta Catenin non-phospho(active) S45 antibody [EPR26155-110-1] (AB316940)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- Alexa Fluor® 647
- Excitation/Emission
- Ex: 650nm, Em: 665nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Not recommended |
| Rat | Tested | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Target data
Function
Key downstream component of the canonical Wnt signaling pathway (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22155184, PubMed:22647378, PubMed:22699938). In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22155184, PubMed:22647378, PubMed:22699938). In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22155184, PubMed:22647378, PubMed:22699938). Involved in the regulation of cell adhesion, as component of an E-cadherin:catenin adhesion complex (By similarity). Acts as a negative regulator of centrosome cohesion (PubMed:18086858). Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization (PubMed:21262353). Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2 (PubMed:18957423). Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML (PubMed:22155184). Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle (By similarity). Involved in chondrocyte differentiation via interaction with SOX9: SOX9-binding competes with the binding sites of TCF/LEF within CTNNB1, thereby inhibiting the Wnt signaling (By similarity). Acts as a positive regulator of odontoblast differentiation during mesenchymal tooth germ formation, via promoting the transcription of differentiation factors such as LEF1, BMP2 and BMP4 (By similarity). Activity is repressed in a MSX1-mediated manner at the bell stage of mesenchymal tooth germ formation which prevents premature differentiation of odontoblasts (By similarity).
Alternative names
CTNNB, OK/SW-cl.35, PRO2286, CTNNB1, Catenin beta-1, Beta-catenin
Recommended products
Knockout Tested Rabbit Recombinant Monoclonal beta Catenin antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- Alexa Fluor® 647
- Excitation/Emission
- Ex: 650nm, Em: 665nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR26155-110-1
- Purification technique
- Affinity purification Protein A
- Specificity
Unsuitable for mouse ICC.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Store in the dark
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Beta Catenin also known by names such as CTNNB1 or beta-chip is an important protein involved in cell signaling and adhesion. This protein has a molecular weight of around 88 kDa. Beta Catenin is expressed in many cell types and tissues indicating its widespread role in various biological processes. It functions mechanically by mediating the linkage between cadherins and the actin cytoskeleton facilitating cell-cell adhesion. Beta Catenin is also a central part of transcription regulation processes in the nucleus.
- Biological function summary
This protein plays roles in both cell adhesion and the regulation of gene expression. Beta Catenin is a critical component of the Wnt signaling pathway where it can form complexes with other proteins to influence gene transcription. In the absence of Wnt signaling beta Catenin levels are low due to its degradation. However when the pathway is active it accumulates in the cytoplasm and eventually translocates to the nucleus where it interacts with TCF/LEF transcription factors to regulate the expression of target genes.
- Pathways
Beta Catenin plays a central role in the Wnt signaling pathway and influences cell fate decisions and cellular proliferation. It acts in concert with proteins such as Dishevelled (DVL) and Axin to coordinate these important biological processes. In the absence of Wnt signaling proteins such as APC and GSK-3β are responsible for beta Catenin degradation keeping its cellular levels in check. Beta Catenin’s interaction with transcription factors in the nucleus makes it pivotal in the regulation of cell and tissue homeostasis.
- Associated diseases and disorders
Beta Catenin has associations with colorectal cancer and hepatocellular carcinoma. Its dysregulation can lead to unchecked cell proliferation and tumorigenesis. Often mutations in the beta Catenin gene (CTNNB1) or components of the Wnt pathway like APC are implicated in the development of these cancers. Its interplay with E-cadherin is important for maintaining tissue architecture and disruptions can lead to invasive cancer phenotypes. Understanding beta Catenin’s role provides insights into therapeutic strategies for these cancers.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-beta Catenin non-phospho(active) S45 antibody [EPR26155-110-1] (ab316940), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-647-beta-catenin-non-phosphoactive-s45-antibody-epr26155-110-1-ab316940--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img428536.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-beta Catenin non-phospho(active) S45 antibody [EPR26155-110-1] (ab316940)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-beta Catenin non-phospho(active) S45 antibody [EPR26155-110-1] (ab316940)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling beta Catenin with ab316940 at 1/100 (5.0 ug/ml) dilution.
Membranous staining on human colon.
The section was incubated with ab316940 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-beta Catenin non-phospho(active) S45 antibody [EPR26155-110-1] (ab316940), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-647-beta-catenin-non-phosphoactive-s45-antibody-epr26155-110-1-ab316940--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img428537.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-beta Catenin non-phospho(active) S45 antibody [EPR26155-110-1] (ab316940)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-beta Catenin non-phospho(active) S45 antibody [EPR26155-110-1] (ab316940)
Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue labeling beta Catenin with ab316940 at 1/100 (5.0 ug/ml) dilution.
Membranous staining on human ovarian cancer.
The section was incubated with ab316940 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-beta Catenin non-phospho(active) S45 antibody [EPR26155-110-1] (ab316940), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-647-beta-catenin-non-phosphoactive-s45-antibody-epr26155-110-1-ab316940--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img428538.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-beta Catenin non-phospho(active) S45 antibody [EPR26155-110-1] (ab316940)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-beta Catenin non-phospho(active) S45 antibody [EPR26155-110-1] (ab316940)
Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling beta Catenin with ab316940 at 1/100 (5.0 ug/ml) dilution.
Membranous staining on mouse colon.
The section was incubated with ab316940 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-beta Catenin non-phospho(active) S45 antibody [EPR26155-110-1] (ab316940), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-647-beta-catenin-non-phosphoactive-s45-antibody-epr26155-110-1-ab316940--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img428724.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-beta Catenin non-phospho(active) S45 antibody [EPR26155-110-1] (ab316940)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-beta Catenin non-phospho(active) S45 antibody [EPR26155-110-1] (ab316940)
Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling beta Catenin with ab316940 at 1/100 (5.0 ug/ml) dilution.
Membranous staining on rat colon.
The section was incubated with ab316940 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-beta Catenin non-phospho(active) S45 antibody [EPR26155-110-1] (ab316940), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-647-beta-catenin-non-phosphoactive-s45-antibody-epr26155-110-1-ab316940--immunocytochemistry-immunofluorescence-img428535.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-beta Catenin non-phospho(active) S45 antibody [EPR26155-110-1] (ab316940)")
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-beta Catenin non-phospho(active) S45 antibody [EPR26155-110-1] (ab316940)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized CTNNB1 (beta Catenin) knockout HAP1 cells labelling beta Catenin with ab316940 at 1/50 (10.0 ug/ml) dilution.
Confocal image showing membranous staining in parental HAP1 cell line and no staining in CTNNB1 (beta Catenin) knockout HAP1 cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution.
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