AB204034

Alexa Fluor® 647 Anti-beta IV Tubulin antibody [EPR16775]

RabMAb
Recombinant
What is this?

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(7 Publications)

Rabbit Recombinant Monoclonal TBB4A antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 7 publications.

View Alternative Names

TUBB4, TUBB5, TUBB4A, Tubulin beta-4A chain, Tubulin 5 beta, Tubulin beta-4 chain

1 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-beta IV Tubulin antibody [EPR16775] (AB204034)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-beta IV Tubulin antibody [EPR16775] (AB204034)

ab204034 staining beta IV Tubulin in SKNSH cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab204034 at 1/100 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in SKNSH cells fixed with 100% methanol (5 min).

Carrier free

Anti-beta IV Tubulin antibody [EPR16775] - BSA and Azide free
Unconjugated

Anti-beta IV Tubulin antibody [EPR16775]
660 APC

APC Anti-beta IV Tubulin antibody [EPR16775]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-beta IV Tubulin antibody [EPR16775]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-beta IV Tubulin antibody [EPR16775]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-beta IV Tubulin antibody [EPR16775]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-beta IV Tubulin antibody [EPR16775]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-beta IV Tubulin antibody [EPR16775]
HRP

HRP Anti-beta IV Tubulin antibody [EPR16775]
578 PE

PE Anti-beta IV Tubulin antibody [EPR16775]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16775

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

Reacts with

Human, Human, Mouse, Rat

Applications

ICC/IF, Flow Cyt (Intra), IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta IV Tubulin also known as TUBB4 is an important component of the microtubule structure within cells. It has a molecular mass of approximately 50 kDa. This protein is a critical part of the cytoskeleton and is expressed in a variety of tissues including neuronal tissues. Beta IV Tubulin plays a role in forming dynamic structures that aid in maintaining cell shape facilitating intracellular transport and enabling cell division. Researchers often use beta tubulin staining techniques to observe microtubules in cells which is important for understanding cellular processes.

Biological function summary

Beta IV Tubulin contributes significantly to the stability and function of microtubule networks. It is a member of the tubulin family and often forms tubulin heterodimers which are building blocks of microtubules. These microtubules are essential for many cellular activities such as mitosis and transport of organelles. Beta IV Tubulin is integral to the microtubule-based cellular processes and its functions are critical for neuronal development and function because it is a part of the complex cytoskeletal framework.

Pathways

Beta IV Tubulin is deeply involved in cellular processes such as the MAP kinase pathway and cell cycle regulation. These pathways are pivotal in transmitting signals that regulate cellular processes including growth and differentiation. The protein interacts with other tubulin isotypes and motor proteins like dynein and kinesin contributing to intracellular transport and chromosomal segregation during cell division. These interactions highlight its importance in maintaining proper cellular responses and functions.

Mutations or dysregulation of beta IV Tubulin are associated with neurological conditions like dystonia and Charcot-Marie-Tooth disease. These disorders underline the importance of beta IV Tubulin in neuronal health and development. The protein’s interaction with other tubulins and motor proteins also relates it to neurodegenerative diseases where cytoskeletal integrity and function are often compromised. Understanding these interactions helps unravel the pathogenic mechanisms of these diseases and can guide therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

See full target information TUBB4A

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Nature 647:517-527 PubMed40866703

2025

Mechanical confinement governs phenotypic plasticity in melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Miranda V Hunter,Eshita Joshi,Sydney Bowker,Emily Montal,Yilun Ma,Young Hun Kim,Zhifan Yang,Laura Tuffery,Zhuoning Li,Eric Rosiek,Alexander Browning,Reuben Moncada,Itai Yanai,Helen Byrne,Mara Monetti,Elisa de Stanchina,Pierre-Jacques Hamard,Richard P Koche,Richard M White

Nature communications 13:4416 PubMed35906230

2022

SARS-CoV-2 VOC type and biological sex affect molnupiravir efficacy in severe COVID-19 dwarf hamster model.

Applications

Unspecified application

Species

Unspecified reactive species

Carolin M Lieber,Robert M Cox,Julien Sourimant,Josef D Wolf,Kate Juergens,Quynh Phung,Manohar T Saindane,Meghan K Smith,Zachary M Sticher,Alexander A Kalykhalov,Michael G Natchus,George R Painter,Kaori Sakamoto,Alexander L Greninger,Richard K Plemper

Science advances 8:eabo2236 PubMed35749502

2022

Orally efficacious lead of the AVG inhibitor series targeting a dynamic interface in the respiratory syncytial virus polymerase.

Applications

Unspecified application

Species

Unspecified reactive species

Julien Sourimant,Carolin M Lieber,Jeong-Joong Yoon,Mart Toots,Mugunthan Govindarajan,Venkata Udumula,Kaori Sakamoto,Michael G Natchus,Joseph Patti,John Vernachio,Richard K Plemper

Cells 11: PubMed35269407

2022

Neutrophil Extracellular Traps Do Not Induce Injury and Inflammation in Well-Differentiated RSV-Infected Airway Epithelium.

Applications

Unspecified application

Species

Unspecified reactive species

Rosalie S N Linssen,Adithya Sridhar,Giulia Moreni,Nicole N van der Wel,Job B M van Woensel,Katja C Wolthers,Reinout A Bem

Nature communications 12:4354 PubMed34272374

2021

SARS-CoV-2 infection induces the dedifferentiation of multiciliated cells and impairs mucociliary clearance.

Applications

Unspecified application

Species

Unspecified reactive species

Rémy Robinot,Mathieu Hubert,Guilherme Dias de Melo,Françoise Lazarini,Timothée Bruel,Nikaïa Smith,Sylvain Levallois,Florence Larrous,Julien Fernandes,Stacy Gellenoncourt,Stéphane Rigaud,Olivier Gorgette,Catherine Thouvenot,Céline Trébeau,Adeline Mallet,Guillaume Duménil,Samy Gobaa,Raphaël Etournay,Pierre-Marie Lledo,Marc Lecuit,Hervé Bourhy,Darragh Duffy,Vincent Michel,Olivier Schwartz,Lisa A Chakrabarti

Current research in toxicology 1:56-69 PubMed34345837

2020

Comparison of the basic morphology and function of 3D lung epithelial cultures derived from several donors.

Applications

Unspecified application

Species

Unspecified reactive species

David Bovard,Albert Giralt,Keyur Trivedi,Laurent Neau,Petros Kanellos,Anita Iskandar,Athanasios Kondylis,Karsta Luettich,Stefan Frentzel,Julia Hoeng,Manuel C Peitsch

SLAS technology 25:247-252 PubMed31971054

2020

In Vitro High-Content Imaging-Based Phenotypic Analysis of Bronchial 3D Organotypic Air-Liquid Interface Cultures.

Applications

Unspecified application

Species

Unspecified reactive species

Diego Marescotti,David Bovard,Moran Morelli,Antonin Sandoz,Karsta Luettich,Stefan Frentzel,Manuel Peitsch,Julia Hoeng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 647 Anti-beta IV Tubulin antibody [EPR16775]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-beta IV Tubulin antibody [EPR16775] (AB204034)

Related conjugates and formulations (10)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise