AB190560

Alexa Fluor® 647 Anti-c-Myc antibody [Y69]

Name: Alexa Fluor® 647 conjugated Anti-c-Myc antibody [Y69] (ab190560) | Abcam
Brand: Abcam Limited
SKU: AB190560
Price: 680 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
KO Validated
What is this?

(1 Review)

(4 Publications)

Anti-c-Myc antibody [Y69] - Alexa Fluor® 647 conjugated (ab190560) is a rabbit recombinant monoclonal antibody detecting c-Myc in ICC/IF. Suitable for Human.

- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

BHLHE39, MYC, Myc proto-oncogene protein, Class E basic helix-loop-helix protein 39, Proto-oncogene c-Myc, Transcription factor p64, bHLHe39

2 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-c-Myc antibody [Y69] (AB190560)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-c-Myc antibody [Y69] (AB190560)

ab190560 staining c-Myc in panc1 cells. The cells were fixed with 100% methanol (5 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab190560 at a working dilution of 1 in 100 (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor^® 488, shown in green) at 2μg/ml overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

This product also gave a positive signal in 4% formaldehyde (10 min) fixed panc1 cells under the same testing conditions.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-c-Myc antibody [Y69] (AB190560)

ab190560 staining c-Myc in wild-type HEK293 cells (top panel) and MYC knockout HEK293 cells (ab256500) (bottom panel). The cells were fixed with 4% paraformaldehyde (10 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab190560 at 1/5000 dilution and ab195887 (Mouse monoclonal to alpha Tubulin - Alexa Fluor^® 488) at 1/250 dilution overnight at 4°C. Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

Carrier free

Anti-c-Myc antibody [Y69] - ChIP Grade - BSA and Azide free
Unconjugated

Anti-c-Myc antibody [Y69] - ChIP Grade
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-c-Myc antibody [Y69]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-c-Myc antibody [Y69]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-c-Myc antibody [Y69]
519 FITC

FITC Anti-c-Myc antibody [Y69]
HRP

HRP Anti-c-Myc antibody [Y69]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

Y69

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

Reacts with

Human, Human, Mouse, Rat

Applications

IHC-P, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody is specific for endogenous c-Myc. It does not detect Myc tag. Expression levels of the target protein vary with sample type and some optimization may be required.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

What is this antibody validated in?
Alexa Fluor® 647 Anti-c-Myc antibody [Y69] (ab190560) is a rabbit recombinant monoclonal antibody and is validated for use in Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

Related products
Antibody clone Y69 is also available pre-conjugated to a variety of labels for your convenience – Anti-c-Myc Alexa Fluor® 647 [Y69] (ab190560).

Specificity confirmed
The specificity of Alexa Fluor® 647 Anti-c-Myc antibody [Y69] (ab190560) has been confirmed by Immunocytochemistry testing in MYC Knockout HEK-293 cell line, ab256500.

Other related products
We have a range of other formats of antibody clone [Y69] also available for your convenience: ab32072, Carrier free - ab168727, Alexa Fluor® 488 - ab190026, Alexa Fluor® 647 - ab190560, Alexa Fluor® 594 - ab201775, Alexa Fluor® 555 - ab201780, HRP - ab205818, FITC - ab223913

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C-Myc also known simply as Myc or MYC protein is a transcription factor with significant roles in cellular processes. Its estimated molecular weight is approximately 62 kDa. This protein is expressed in various tissues and cell types including common use in HEK (human embryonic kidney) cells for research. c-Myc functions by regulating transcription of genes involved in cell cycle progression apoptosis and cellular transformation.

Biological function summary

C-Myc is involved in regulating cell growth and proliferation. It forms part of the Myc/Max complex which binds to DNA to regulate gene expression. This activity affects cellular metabolism ribosome biogenesis and cell cycle entry emphasizing its regulation of cellular energy and stress response. Its expression levels critically govern normal cellular functions and homeostasis.

Pathways

C-Myc plays an important role in the cell cycle pathway and apoptosis regulation. Specifically c-Myc is associated with the Wnt signaling pathway which influences cellular proliferation and differentiation. It also interacts with other proteins like Cyclin D1 influencing cell cycle control. These interactions ensure c-Myc's involvement in regulating key processes related to cell proliferation and stability.

C-Myc is tightly linked to cancer such as Burkitt's lymphoma and colon cancer. In these conditions c-Myc overexpression contributes to uncontrolled cell proliferation. Additionally c-Myc is associated with other oncogenic proteins like BCL2 in tumorigenesis highlighting its pivotal role in cancer development and progression. Understanding c-Myc's involvement in these diseases aids in the development of targeted therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3' (PubMed : 24940000, PubMed : 25956029). Activates the transcription of growth-related genes (PubMed : 24940000, PubMed : 25956029). Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis (PubMed : 24940000, PubMed : 25956029). Regulator of somatic reprogramming, controls self-renewal of embryonic stem cells (By similarity). Functions with TAF6L to activate target gene expression through RNA polymerase II pause release (By similarity). Positively regulates transcription of HNRNPA1, HNRNPA2 and PTBP1 which in turn regulate splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform (PubMed : 20010808).

See full target information MYC

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Proceedings of the National Academy of Sciences of the United States of America 121:e2322068121 PubMed39042692

2024

Spatial molecular profiling of mixed invasive ductal and lobular breast cancers reveals heterogeneity in intrinsic molecular subtypes, oncogenic signatures, and mutations.

Applications

Unspecified application

Species

Unspecified reactive species

Osama Shiraz Shah,Azadeh Nasrazadani,Julia Foldi,Jennifer M Atkinson,Celina G Kleer,Priscilla F McAuliffe,Tyler J Johnston,Wayne Stallaert,Edaise M da Silva,Pier Selenica,Higinio Dopeso,Fresia Pareja,Diana Mandelker,Britta Weigelt,Jorge S Reis-Filho,Rohit Bhargava,Peter C Lucas,Adrian V Lee,Steffi Oesterreich

Science (New York, N.Y.) 379:eabj7412 PubMed36656933

2023

mutation yields supercompetitive B cells primed for malignant transformation.

Applications

Unspecified application

Species

Unspecified reactive species

Coraline Mlynarczyk,Matt Teater,Juhee Pae,Christopher R Chin,Ling Wang,Theinmozhi Arulraj,Darko Barisic,Antonin Papin,Kenneth B Hoehn,Ekaterina Kots,Jonatan Ersching,Arnab Bandyopadhyay,Ersilia Barin,Hui Xian Poh,Chiara M Evans,Amy Chadburn,Zhengming Chen,Hao Shen,Hannah M Isles,Benedikt Pelzer,Ioanna Tsialta,Ashley S Doane,Huimin Geng,Muhammad Hassan Rehman,Jonah Melnick,Wyatt Morgan,Diu T T Nguyen,Olivier Elemento,Michael G Kharas,Samie R Jaffrey,David W Scott,George Khelashvili,Michael Meyer-Hermann,Gabriel D Victora,Ari Melnick

eLife 9: PubMed33051000

2020

Critical role of WNK1 in MYC-dependent early mouse thymocyte development.

Applications

Unspecified application

Species

Unspecified reactive species

Robert Köchl,Lesley Vanes,Miriam Llorian Sopena,Probir Chakravarty,Harald Hartweger,Kathryn Fountain,Andrea White,Jennifer Cowan,Graham Anderson,Victor Lj Tybulewicz

The international journal of biochemistry & cell biology 106:84-95 PubMed30453092

2018

Direct conversion of mouse embryonic fibroblast to osteoblast cells using hLMP-3 with Yamanaka factors.

Applications

Unspecified application

Species

Unspecified reactive species

Mahmoud F Ahmed,Ahmed Kamel El-Sayed,Hao Chen,Ruifeng Zhao,Kai Jin,Qisheng Zuo,Yani Zhang,Bichun Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 647 Anti-c-Myc antibody [Y69]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-c-Myc antibody [Y69] (AB190560)

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-c-Myc antibody [Y69] (AB190560)

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Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Properties and storage information

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Shipped at conditions

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Appropriate short-term storage conditions

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Aliquoting information

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Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise