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AB307519

Alexa Fluor® 647 Anti-C Reactive Protein antibody [Y284]

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Rabbit Recombinant Monoclonal C Reactive Protein antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-P and reacts with Human samples.

View Alternative Names

PTX1, CRP, C-reactive protein

3 Images
Immunohistochemistry - Alexa Fluor® 647 Anti-C Reactive Protein antibody [Y284] (AB307519)
  • IHC

Supplier Data

Immunohistochemistry - Alexa Fluor® 647 Anti-C Reactive Protein antibody [Y284] (AB307519)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling C Reactive Protein with ab307519 at 1/100 (5.0 ug/ml). Negative control : no staining on human skeletal muscle. The section was incubated with ab307519 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND RX instrument.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

Immunohistochemistry - Alexa Fluor® 647 Anti-C Reactive Protein antibody [Y284] (AB307519)
  • IHC

Supplier Data

Immunohistochemistry - Alexa Fluor® 647 Anti-C Reactive Protein antibody [Y284] (AB307519)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling C Reactive Protein with ab307519 at 1/100 (5.0 ug/ml). Cytoplasmic staining on human liver. The section was incubated with ab307519 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

Immunohistochemistry - Alexa Fluor® 647 Anti-C Reactive Protein antibody [Y284] (AB307519)
  • IHC

Supplier Data

Immunohistochemistry - Alexa Fluor® 647 Anti-C Reactive Protein antibody [Y284] (AB307519)

Immunohistochemical analysis of paraffin-embedded Human hepatocellular tissue labeling C Reactive Protein with ab307519 at 1/100 (5.0 ug/ml). Cytoplasmic staining on human hepatocellular carcinoma. The section was incubated with ab307519 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

  • Unconjugated

    Anti-C Reactive Protein antibody [Y284]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-C Reactive Protein antibody [Y284]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-C Reactive Protein antibody [Y284]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-C Reactive Protein antibody [Y284]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-C Reactive Protein antibody [Y284]

  • Carrier free

    Anti-C Reactive Protein antibody [Y284] - BSA and Azide free

  • HRP

    HRP Anti-C Reactive Protein antibody [Y284]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-C Reactive Protein antibody [Y284]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

Y284

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" } } }
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You may be interested in:

AB99995

Human C Reactive Protein ELISA Kit (CRP)

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View product

We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C-reactive protein (CRP) also known as CRP protein is an acute-phase protein with a molecular mass of approximately 25 kDa. It is primarily synthesized in the liver in response to cytokines. This protein appears in the blood where it binds to phosphocholine expressed on the surface of dead or dying cells. CRP is often used as a biomarker for inflammation in the body and its levels in human and canine (anti CRP used) blood samples are measured using assays like the CRP ELISA test or CRP ELISA kit.
Biological function summary

CRP plays a significant role in the innate immune system by recognizing foreign pathogens and damaged cells. It does not form part of a larger complex but itself can bind to specific ligands initiating the complement cascade. This activation enhances phagocytosis by macrophages contributing to the clearance of necrotic and apoptotic cells. The CRP protein therefore acts as a fast responder in the presence of inflammation making it a useful indicator for monitoring inflammatory diseases.

Pathways

CRP operates predominantly within the acute-phase response pathway. Its production is upregulated by cytokines such as IL-6 which connects it to inflammation-signaling pathways. CRP also interacts with the complement system bridging innate and adaptive immunity. Besides CRP could indirectly influence pathways involving other inflammatory proteins like serum amyloid A and haptoglobin creating a network of responses important for managing inflammation.

Elevated CRP levels are commonly associated with cardiovascular diseases and rheumatoid arthritis. In cardiovascular diseases CRP acts as a marker for atherosclerosis where the protein levels correlate with disease severity. It connects with other proteins involved in inflammatory processes such as fibrinogen to contribute to disease pathology. In rheumatoid arthritis CRP provides insights into disease activity helping in the assessment of treatment responses.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Displays several functions associated with host defense : it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
See full target information CRP
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Version
Primary Antibodies

AB157242

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Alternative Version
Primary Antibodies

AB271830

Anti-C Reactive Protein antibody [Y284] - BSA and Azide free

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Alternative Version
Primary Antibodies

AB314471

Alexa Fluor® 488 Anti-C Reactive Protein antibody [Y284]

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