Rabbit Recombinant Monoclonal CaMKII delta antibody - conjugated to Alexa Fluor® 647.
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Antibody Labelling | Reactivity Expected | Dilution info - | Notes - |
Application Target Binding Affinity | Reactivity Expected | Dilution info - | Notes - |
Calcium/calmodulin-dependent protein kinase involved in the regulation of Ca(2+) homeostatis and excitation-contraction coupling (ECC) in heart by targeting ion channels, transporters and accessory proteins involved in Ca(2+) influx into the myocyte, Ca(2+) release from the sarcoplasmic reticulum (SR), SR Ca(2+) uptake and Na(+) and K(+) channel transport. Targets also transcription factors and signaling molecules to regulate heart function. In its activated form, is involved in the pathogenesis of dilated cardiomyopathy and heart failure. Contributes to cardiac decompensation and heart failure by regulating SR Ca(2+) release via direct phosphorylation of RYR2 Ca(2+) channel on 'Ser-2808'. In the nucleus, phosphorylates the MEF2 repressor HDAC4, promoting its nuclear export and binding to 14-3-3 protein, and expression of MEF2 and genes involved in the hypertrophic program (PubMed:17179159). Is essential for left ventricular remodeling responses to myocardial infarction. In pathological myocardial remodeling acts downstream of the beta adrenergic receptor signaling cascade to regulate key proteins involved in ECC. Regulates Ca(2+) influx to myocytes by binding and phosphorylating the L-type Ca(2+) channel subunit beta-2 CACNB2. In addition to Ca(2+) channels, can target and regulate the cardiac sarcolemmal Na(+) channel Nav1.5/SCN5A and the K+ channel Kv4.3/KCND3, which contribute to arrhythmogenesis in heart failure. Phosphorylates phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2, contributing to the enhancement of SR Ca(2+) uptake that may be important in frequency-dependent acceleration of relaxation (FDAR) and maintenance of contractile function during acidosis (PubMed:16690701). May participate in the modulation of skeletal muscle function in response to exercise, by regulating SR Ca(2+) transport through phosphorylation of PLN/PLB and triadin, a ryanodine receptor-coupling factor. In response to interferon-gamma (IFN-gamma) stimulation, catalyzes phosphorylation of STAT1, stimulating the JAK-STAT signaling pathway (By similarity).
CAMKD, CAMK2D, Calcium/calmodulin-dependent protein kinase type II subunit delta, CaM kinase II subunit delta, CaMK-II subunit delta
Rabbit Recombinant Monoclonal CaMKII delta antibody - conjugated to Alexa Fluor® 647.
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
The mouse and rat recommendation (on parent abid) is based on the WB results. We do not guarantee IHC-P for mouse and rat.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
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This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone. This conjugated antibody is eligible for the Abcam trial program.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
CaMKII delta also known as CaMKIIδ or CaMK2D is a member of the CaMKII family of proteins recognized for its role as a serine/threonine protein kinase. This protein has a molecular mass of approximately 55 kDa. It is highly expressed in the brain and the heart where it plays essential roles in cellular functions. The domain structure of CaMKII delta allows for calcium/calmodulin activation which is important for its activity in phosphorylating substrates. This protein can bind to other intracellular molecules and is sensitive to regulation by its surrounding environment.
CaMKII delta participates in several critical processes such as calcium signaling and regulation of gene expression. This protein influences synaptic plasticity muscle contraction and cell cycle control. It forms part of larger complexes interacting with other proteins and playing a role in adjusting cellular responses to various stimuli. It is also subject to inhibition with the compound Bisindolylmaleimide II known to influence its activity. Understanding the biological roles of CaMKII delta allows for insights into its effects on cellular mechanisms especially in neuronal and muscular tissues.
CaMKII delta is significant in pathways like the calcium signaling pathway and the PI3K/Akt pathway. It serves as a modulator allowing the integration of various signals which is necessary for maintaining cellular homeostasis. CaMKII delta interacts with proteins such as protein kinase C (PKC) inhibitors affecting cellular signaling outputs. Its role in these pathways highlights the importance of CaMKII delta in regulating processes critical to cell survival growth and adaptation.
CaMKII delta is associated with conditions such as cardiac hypertrophy and Alzheimer's disease. These diseases often involve dysregulation of calcium signaling where CaMKII delta plays a part. CaMKII delta's association with proteins like PKC and their dysregulation contributes to the pathogenesis observed in these conditions. Understanding these interactions can aid in developing therapeutic strategies focused on modulating CaMKII delta activity to mitigate disease progression.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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