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AB225128

Alexa Fluor® 647 Anti-Carbonic anhydrase 2/CA2 antibody [EPR5195]

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(1 Publication)

Rabbit Recombinant Monoclonal CAH2 antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Carbonic anhydrase 2, Carbonate dehydratase II, Carbonic anhydrase C, Carbonic anhydrase II, Cyanamide hydratase CA2, CAC, CA-II, CA2

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Carbonic anhydrase 2/CA2 antibody [EPR5195] (AB225128)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Carbonic anhydrase 2/CA2 antibody [EPR5195] (AB225128)

IHC image of Carbonic anhydrase 2/CA2 staining in a section of formalin-fixed paraffin-embedded normal human stomach*.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) in a Biocare Medical NxGen pressure cooker using retrieval settings of 110oC for 20 minutes. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab225128 at 1/5000 dilution (shown in red) and counterstained using ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Carbonic anhydrase 2/CA2 antibody [EPR5195]

  • Unconjugated

    Anti-Carbonic anhydrase 2/CA2 antibody [EPR5195]

  • Carrier free

    Anti-Carbonic anhydrase 2/CA2 antibody [EPR5195] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5195

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the reversible hydration of carbon dioxide (PubMed : 11327835, PubMed : 11802772, PubMed : 11831900, PubMed : 12056894, PubMed : 12171926, PubMed : 1336460, PubMed : 14736236, PubMed : 15300855, PubMed : 15453828, PubMed : 15667203, PubMed : 15865431, PubMed : 16106378, PubMed : 16214338, PubMed : 16290146, PubMed : 16686544, PubMed : 16759856, PubMed : 16807956, PubMed : 17127057, PubMed : 17251017, PubMed : 17314045, PubMed : 17330962, PubMed : 17346964, PubMed : 17540563, PubMed : 17588751, PubMed : 17705204, PubMed : 18024029, PubMed : 18162396, PubMed : 18266323, PubMed : 18374572, PubMed : 18481843, PubMed : 18618712, PubMed : 18640037, PubMed : 18942852, PubMed : 1909891, PubMed : 1910042, PubMed : 19170619, PubMed : 19186056, PubMed : 19206230, PubMed : 19520834, PubMed : 19778001, PubMed : 7761440, PubMed : 7901850, PubMed : 8218160, PubMed : 8262987, PubMed : 8399159, PubMed : 8451242, PubMed : 8485129, PubMed : 8639494, PubMed : 9265618, PubMed : 9398308). Can also hydrate cyanamide to urea (PubMed : 10550681, PubMed : 11015219). Stimulates the chloride-bicarbonate exchange activity of SLC26A6 (PubMed : 15990874). Essential for bone resorption and osteoclast differentiation (PubMed : 15300855). Involved in the regulation of fluid secretion into the anterior chamber of the eye. Contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption.
See full target information CA2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature 646:676-686 PubMed40770097

2025

Microglia regulate GABAergic neurogenesis in prenatal human brain through IGF1.

Applications

Unspecified application

Species

Unspecified reactive species

Diankun Yu,Samhita Jain,Andi Wangzhou,Beika Zhu,Wenyuan Shao,Elena J Coley-O'Rourke,Stacy De Florencio,JaeYeon Kim,Jennifer Ja-Yoon Choi,Mercedes F Paredes,Tomasz J Nowakowski,Eric J Huang,Xianhua Piao
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com