AB225074

Alexa Fluor® 647 Anti-Carbonic Anhydrase 9/CA9 antibody [EPR4151(2)]

BOND RX™ Validated
RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal Carbonic Anhydrase 9 antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications.

View Alternative Names

G250, MN, CA9, Carbonic anhydrase 9, Carbonate dehydratase IX, Carbonic anhydrase IX, Membrane antigen MN, P54/58N, Renal cell carcinoma-associated antigen G250, pMW1, CA-IX, CAIX, RCC-associated antigen G250

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Carbonic Anhydrase 9/CA9 antibody [EPR4151(2)] (AB225074)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Carbonic Anhydrase 9/CA9 antibody [EPR4151(2)] (AB225074)

IHC image of Carbonic Anhydrase 9/CA9 staining in a section of formalin-fixed paraffin-embedded normal human stomach*.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins performed on a Leica BOND™. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab225074 at 1/100 dilution (shown in red) and counterstained using ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount^®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Carbonic Anhydrase 9/CA9 antibody [EPR4151(2)]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Carbonic Anhydrase IX antibody [EPR4151(2)]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-Carbonic Anhydrase 9/CA9 antibody [EPR4151(2)]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Carbonic Anhydrase IX antibody [EPR4151(2)]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-Carbonic Anhydrase 9/CA9 antibody [EPR4151(2)]
Unconjugated

Anti-Carbonic Anhydrase 9/CA9 antibody [EPR4151(2)]
Carrier free

Anti-Carbonic Anhydrase 9/CA9 antibody [EPR4151(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4151(2)

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

Reacts with

Human, Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the interconversion between carbon dioxide and water and the dissociated ions of carbonic acid (i.e. bicarbonate and hydrogen ions).

See full target information CA9

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nanoscale advances 6:276-286 PubMed38125591

2023

Lithographically defined encoded magnetic heterostructures for the targeted screening of kidney cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Selma Leulmi Pichot,Tarun Vemulkar,Jeroen Verheyen,Lauren Wallis,James O Jones,Andrew P Stewart,Sarah J Welsh,Grant D Stewart,Russell P Cowburn

The FEBS journal 289:2895-2914 PubMed34839586

2021

Biogenesis of hepatitis B virus e antigen is driven by translocon-associated protein complex and regulated by conserved cysteine residues within its signal peptide sequence.

Applications

Unspecified application

Species

Unspecified reactive species

Helena Zábranská,Aleš Zábranský,Barbora Lubyová,Jan Hodek,Alena Křenková,Martin Hubálek,Jan Weber,Iva Pichová

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 647 Anti-Carbonic Anhydrase 9/CA9 antibody [EPR4151(2)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Carbonic Anhydrase 9/CA9 antibody [EPR4151(2)] (AB225074)

Related conjugates and formulations (7)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (2)

Product promise